Journal: Nutrients

Article Title: Punicic Acid Triggers Ferroptotic Cell Death in Carcinoma Cells

doi: 10.3390/nu13082751

Figure Lengend Snippet: Punicic acid (PunA) decreases the viability of HCT-116 and FaDu carcinoma cells and its effect arises from a lower dose as compared to docosahexaenoic acid (DHA). ( a ) Cytotoxic effect of increasing concentrations of PunA on HCT-116 and ( b ) FaDu carcinoma cells after 72 h; ( c ) Kinetics of the cytotoxicity of PunA 14 µM over 72 h on HCT-116 and ( d ) FaDu carcinoma cells; ( e ) Comparison of the cytotoxicity of PunA and DHA at 14 µM on HCT-116 and ( f ) FaDu carcinoma cells applied during 72 h; ( g ) Comparison of the diameter growth of HCT-116 or ( h ) FaDu 3D spheroids under PunA 14 µM or DHA 100 µM. Control: cells or spheroids supplemented with DMEM cell culture medium without added fatty acid. Results are expressed as mean ± standard error of the mean of three independent repetitions. Significance is established in relation to the control ( a , b , e – h ), to PunA 14 µM at 0 h of treatment ( c , d ) or between DHA 100 µM and PunA 14 µM ( g – h ). *** p ≤ 0.0001.

Article Snippet: After 24 h, cell culture medium was supplemented with fixed concentrations of BSA-conjugated punicic acid (Larodan, Solna, Sweden, 10-1875) or docosahexaenoic acid (Larodan, Solna, Sweden, 10-2206), as well as of (+)-α-tocopherol (Sigma, Saint-Louis, MO, USA), ferrostatin-1 (Sigma, Saint-Louis, MO, USA), deferoxamine mesylate (Sigma, Saint-Louis, MO, USA), ZVAD-fmk (Sigma, Saint-Louis, MO, USA) or necrostatin-1 (Sigma, Saint-Louis, MO, USA) for 72 h. All compounds were dissolved in DMSO, except (+)-α-tocopherol, which was dissolved in ethanol.

Techniques: Cell Culture

Journal: Nutrients

Article Title: Punicic Acid Triggers Ferroptotic Cell Death in Carcinoma Cells

doi: 10.3390/nu13082751

Figure Lengend Snippet: Combining punicic acid (PunA) with docosahexaenoic acid (DHA) increases its cytotoxicity on HCT-116 and FaDu carcinoma cells. ( a ) Viability of HCT-116 and ( b ) FaDu carcinoma cells treated with DHA, PunA or combination thereof. ( c ) Diameter growth of HCT-116 and ( d ) FaDu 3D spheroids treated with PunA 14 µM, DHA 100 µM or combination thereof. Control: cells or spheroids supplemented with DMEM cell culture medium without added fatty acid(s). Results are expressed as mean ± standard error of the mean of three independent repetitions. Significance is established in relation to the control or between DHA or PunA alone and their combination. *** p ≤ 0.0001.

Article Snippet: After 24 h, cell culture medium was supplemented with fixed concentrations of BSA-conjugated punicic acid (Larodan, Solna, Sweden, 10-1875) or docosahexaenoic acid (Larodan, Solna, Sweden, 10-2206), as well as of (+)-α-tocopherol (Sigma, Saint-Louis, MO, USA), ferrostatin-1 (Sigma, Saint-Louis, MO, USA), deferoxamine mesylate (Sigma, Saint-Louis, MO, USA), ZVAD-fmk (Sigma, Saint-Louis, MO, USA) or necrostatin-1 (Sigma, Saint-Louis, MO, USA) for 72 h. All compounds were dissolved in DMSO, except (+)-α-tocopherol, which was dissolved in ethanol.

Techniques: Cell Culture