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A: The HTM cells were treated with 500 nmol/L <t>dexamethasone</t> <t>(DEX)</t> for 7d. Cell lysates are subjected to Western blotting to detect polyclonal myocilin (MYOC) proteins. Quantitative assessment of the intensity of each band was performed by densitometry. The data indicate as the means±SDs (n=5). aP<0.01 using the Mann-Whitney U test. B: HTM cells are incubated with pHrodo Escherichia coli bioparticles for 3h (green). Cell nuclei are counterstained by Hoechst 33258 (blue) and observed by fluorescence microscope at 20× magnification (n=4). Scale bar: 100 µmol/L.
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A: The HTM cells were treated with 500 nmol/L <t>dexamethasone</t> <t>(DEX)</t> for 7d. Cell lysates are subjected to Western blotting to detect polyclonal myocilin (MYOC) proteins. Quantitative assessment of the intensity of each band was performed by densitometry. The data indicate as the means±SDs (n=5). aP<0.01 using the Mann-Whitney U test. B: HTM cells are incubated with pHrodo Escherichia coli bioparticles for 3h (green). Cell nuclei are counterstained by Hoechst 33258 (blue) and observed by fluorescence microscope at 20× magnification (n=4). Scale bar: 100 µmol/L.
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Image Search Results


A: The HTM cells were treated with 500 nmol/L dexamethasone (DEX) for 7d. Cell lysates are subjected to Western blotting to detect polyclonal myocilin (MYOC) proteins. Quantitative assessment of the intensity of each band was performed by densitometry. The data indicate as the means±SDs (n=5). aP<0.01 using the Mann-Whitney U test. B: HTM cells are incubated with pHrodo Escherichia coli bioparticles for 3h (green). Cell nuclei are counterstained by Hoechst 33258 (blue) and observed by fluorescence microscope at 20× magnification (n=4). Scale bar: 100 µmol/L.

Journal: International Journal of Ophthalmology

Article Title: Extracellular matrix gene expression in human trabecular meshwork cells following mechanical fluid flow stimulation

doi: 10.18240/ijo.2022.03.03

Figure Lengend Snippet: A: The HTM cells were treated with 500 nmol/L dexamethasone (DEX) for 7d. Cell lysates are subjected to Western blotting to detect polyclonal myocilin (MYOC) proteins. Quantitative assessment of the intensity of each band was performed by densitometry. The data indicate as the means±SDs (n=5). aP<0.01 using the Mann-Whitney U test. B: HTM cells are incubated with pHrodo Escherichia coli bioparticles for 3h (green). Cell nuclei are counterstained by Hoechst 33258 (blue) and observed by fluorescence microscope at 20× magnification (n=4). Scale bar: 100 µmol/L.

Article Snippet: Dexamethasone (DEX) and Y-27632 were bought from Merck Millipore (Darmstadt, Germany).

Techniques: Western Blot, MANN-WHITNEY, Incubation, Fluorescence, Microscopy