Journal: Molecular metabolism

Article Title: Adipocyte glucocorticoid receptor is important in lipolysis and insulin resistance due to exogenous steroids, but not insulin resistance caused by high fat feeding.

doi: 10.1016/j.molmet.2017.06.013

Figure Lengend Snippet: Figure 2: Dexamethasone administration increases adiposity independently of GR in adipocytes. Flox and AGRKO mice were injected with saline or dexamethasone (Dex, 3 mg/kg body weight) every other day for two months (n ¼ 8e9 per group). (A) Body weight, (B) adipose tissue weights, and (C) histology (H&E staining) of eWAT and iWAT of Flox and AGRKO mice injected with saline or Dex. (D) The distribution of adipocyte size calculated using ImageJ software. Data are shown as mean SEM. Scale bars indicate 100 mm.

Article Snippet: For studies using dexamethasone (Dex), 16-week old littermate Flox and AGRKO mice were administered saline or dexamethasone sodium phosphate (3 mg/kg body weight; Santa Cruz Biotechnology) intraperitoneally every other day for 2 months.

Techniques: Injection, Saline, Staining, Software

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Analysis of the Sphingolipidome in NAFLD

doi: 10.1007/978-1-0716-2128-8_22

Figure Lengend Snippet: Supplemented William’s E.

Article Snippet: Supplemented William’s E Media, prepare according to , pre-warmed to 37 °C. table ft1 table-wrap mode="anchored" t5 Table 5: caption a7 William’s E Media 437.5 ml Pen/Strep (10k U/ml) 5 ml L-Glutamine (200 μM) 5 mL Hepes (1M, pH 7.4) 7.5 mL Insulin (Santa Cruz sc-360248) 58 μL Dexamethasone 25 μl FBS 50 mL Open in a separate window Supplemented William’s E. 10x Balanced Salt Solution, prepare according to . table ft1 table-wrap mode="anchored" t5 Table 6: caption a7 NaCl 80 g KCl 4 g MgSO 4 2 g ddH 2 O 1 L Open in a separate window 10x Balanced Salt Solution.

Techniques: