Journal: Applied Biochemistry and Biotechnology

Article Title: Immunomodulatory Effects of Huaier Extract on Th1/Th2 Polarization Through IL-33/ST2 Axis Regulation in Allergic Rhinitis Pathogenesis

doi: 10.1007/s12010-026-05706-w

Figure Lengend Snippet: Experimental design for murine allergic rhinitis induction (panels A-B ). Huaier extract reduced clinical symptoms and nasal mucosal inflammation. ( C ) Clinical rhinitis symptom scores. Bar colors correspond to experimental groups as follows: dark blue, Control; light blue, OVA (Model); dark red, OVA + Huaier; light red, OVA + Dexamethasone. ( D ) Nasal mucosal structural alterations assessed by H&E staining (top row: full-tissue views). Abbreviations: ns: No significance. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. Control; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. Model group. (All subsequent figures use identical statistical annotations)

Article Snippet: Dexamethasone phosphate disodium (MedChemExpress, Shanghai, China; cat. no. HY-B1829A).

Techniques: Control, Staining

Journal: Applied Biochemistry and Biotechnology

Article Title: Immunomodulatory Effects of Huaier Extract on Th1/Th2 Polarization Through IL-33/ST2 Axis Regulation in Allergic Rhinitis Pathogenesis

doi: 10.1007/s12010-026-05706-w

Figure Lengend Snippet: Huaier extract restored Th1/Th2 balance. ( A-F ) Serum cytokine levels (IFN-γ, IL-2, IL-12, IL-4, IL-5) and OVA-sIgE were quantified by ELISA. Bar colors correspond to experimental groups as follows: dark blue, Control; light blue, OVA (Model); dark red, OVA + Huaier; light red, OVA + Dexamethasone

Article Snippet: Dexamethasone phosphate disodium (MedChemExpress, Shanghai, China; cat. no. HY-B1829A).

Techniques: Enzyme-linked Immunosorbent Assay, Control

Journal: Applied Biochemistry and Biotechnology

Article Title: Immunomodulatory Effects of Huaier Extract on Th1/Th2 Polarization Through IL-33/ST2 Axis Regulation in Allergic Rhinitis Pathogenesis

doi: 10.1007/s12010-026-05706-w

Figure Lengend Snippet: Nasal tissue expression of IL-2 and IL-12. ( A ) Group comparison of IL-2 and IL-12 protein levels and tissue distribution via immunohistochemistry. ( B ) Quantitative analysis of IL-2 immunostaining intensity. ( C ) Quantitative analysis of IL-12 immunostaining intensity. Bar colors correspond to experimental groups as follows: dark blue, Control; light blue, OVA (Model); dark red, OVA + Huaier; light red, OVA + Dexamethasone

Article Snippet: Dexamethasone phosphate disodium (MedChemExpress, Shanghai, China; cat. no. HY-B1829A).

Techniques: Expressing, Comparison, Immunohistochemistry, Immunostaining, Control

Journal: Applied Biochemistry and Biotechnology

Article Title: Immunomodulatory Effects of Huaier Extract on Th1/Th2 Polarization Through IL-33/ST2 Axis Regulation in Allergic Rhinitis Pathogenesis

doi: 10.1007/s12010-026-05706-w

Figure Lengend Snippet: IL-5 and IL-13 expression in nasal tissues. ( A ) Immunohistochemical detection of IL-5 and IL-13 protein distribution across experimental groups. ( B ) Quantified IL-5 immunostaining intensity. ( C ) IL-13 immunostaining intensity measurements. Bar colors correspond to experimental groups as follows: dark blue, Control; light blue, OVA (Model); dark red, OVA + Huaier; light red, OVA + Dexamethasone

Article Snippet: Dexamethasone phosphate disodium (MedChemExpress, Shanghai, China; cat. no. HY-B1829A).

Techniques: Expressing, Immunohistochemical staining, Immunostaining, Control

Journal: Applied Biochemistry and Biotechnology

Article Title: Immunomodulatory Effects of Huaier Extract on Th1/Th2 Polarization Through IL-33/ST2 Axis Regulation in Allergic Rhinitis Pathogenesis

doi: 10.1007/s12010-026-05706-w

Figure Lengend Snippet: IL-33 and ST2 expression in nasal tissues. ( A ) Immunohistochemical analysis of IL-33 cytokine and ST2 distribution across experimental groups. ( B ) Quantitative IL-33 immunostaining intensity. ( C ) ST2 immunostaining intensity measurements. Bar colors correspond to experimental groups as follows: dark blue, Control; light blue, OVA (Model); dark red, OVA + Huaier; light red, OVA + Dexamethasone

Article Snippet: Dexamethasone phosphate disodium (MedChemExpress, Shanghai, China; cat. no. HY-B1829A).

Techniques: Expressing, Immunohistochemical staining, Immunostaining, Control

Journal: Journal of Biological Chemistry

Article Title: Krüppel-like Factor 13 Is a Major Mediator of Glucocorticoid Receptor Signaling in Cardiomyocytes and Protects These Cells from DNA Damage and Death

doi: 10.1074/jbc.m116.725903

Figure Lengend Snippet: FIGURE 4. KLF13 is a direct target of the glucocorticoid receptor in HL-1 mouse adult cardiomyocytes. A, HL-1 cells were treated with vehicle (control, white bars), 100 nM Dex (black bars), 1 M RU486 (striped bars), or both 1 M RU486 and 100 nM Dex (cross-hatched bars). KLF13 mRNA were measured by RT-PCR at the indicated times. B, HL-1 cells transfected with NTC siRNA or GR siRNA (GR) were treated with 100 nM Dex for 3 h. mRNA levels were measured by RT-PCR. C, HL-1 cells were pretreated for 1–6 h with vehicle (control) or 10 g/ml of cycloheximide (cyclo) then cells were exposed to 100 nM Dex for 3 h. KLF13 mRNA levels were assessed by RT-PCR. D, nascent RNA was measured by RT-PCR after treating the cells for 3 h with 100 nM Dex or vehicle (control). Data represent mean S.E. from three or four independent experiments. *, p 0.05; **, p 0.01; ***, p 0.001.

Article Snippet: Reagents and Antibodies—Dexamethasone and mifepristone (RU486) were purchased from Steraloids (Newport, RI).

Techniques: Control, Reverse Transcription Polymerase Chain Reaction, Transfection

Journal: Journal of Biological Chemistry

Article Title: Krüppel-like Factor 13 Is a Major Mediator of Glucocorticoid Receptor Signaling in Cardiomyocytes and Protects These Cells from DNA Damage and Death

doi: 10.1074/jbc.m116.725903

Figure Lengend Snippet: FIGURE5.GlucocorticoidreceptorisrecruitedtoafunctionalGRElocatedintheintronoftheKLF13geneinvitroandinvivo.A,schematicrepresentation of the KLF13 gene. Sequence alignment of the consensus GRE and GRE identified within the KLF13 intron of the mouse and human genes. HL-1 cells were treated with vehicle-control (white bars) or 100 nM Dex (black bars) for 3 h, and ChIP assays were performed with equivalent amounts of rabbit IgG or rabbit anti-GR antibody. Coimmunoprecipitated DNA was analyzed by RT-PCR using primers to the KLF13 promoter, the KLF13 intron (Int-KLF13) and a functional GRE previously identified in the promoter of the glucocorticoid-regulated gene leucine zipper (GILZ) (left panel). Adrenalectomized C57BL/6 male mice were injectedwithvehicle(Veh,whitebars)or1mg/kgofDex(blackbars).Sixhafterthistreatmentheartswereharvested.CoimmunoprecipitatedDNAwasanalyzed by RT-PCR using primers to the KLF13 intron (Int-KLF13) and GILZ promoter GRE (right panel). Results are plotted as a function of input DNA. B and C, HL-1 cells were transfected with the following luciferase reporter plasmids: control vector (empty vector), KLF13 promoter, intron-KLF13 (Int-KLF13), and mutant-KLF13 (Mut-KLF13). Twenty-four h after transfection cells were treated for 18 h with vehicle control (white bars), 100 nM Dex (black bars), 1 M RU486 (striped bars) or both 1 M RU486 and 100 nM Dex (cross-hatched bars). Cells were harvested and luciferase activity was measured. Data represent S.E. for four independent experiments and n 5–10 mice per group. *, p 0.05; **, p 0.01.

Article Snippet: Reagents and Antibodies—Dexamethasone and mifepristone (RU486) were purchased from Steraloids (Newport, RI).

Techniques: Sequencing, Control, Reverse Transcription Polymerase Chain Reaction, Functional Assay, Transfection, Luciferase, Plasmid Preparation, Mutagenesis, Activity Assay

Journal: Frontiers in Physiology

Article Title: Thiamet-G facilitates reparative dentin formation via modulating O-GlcNAcylation and inflammation

doi: 10.3389/fphys.2025.1739168

Figure Lengend Snippet: Effect of Thiamet-G on human dental pulp stem cells (hDPSCs). (A) Experimental design for MTS assay to examine cell viability and proliferation. (B) MTS assay shows that Thiamet-G treatment facilitates dose-dependent hDPSC proliferation. (C) Experimental design for ALP activity assay. (D) Thiamet-G treatment significantly induces ALP activity in hDPSCs compared to the control, particularly after 14 days (E) Thiamet-G treatment increases the expression of reparative dentin-related markers, including Alp, Bmp2, Bsp, Dspp, Opn, Gsk3β, and RUNX2, compared to the control. Ns non-significant, * p < 0.03, ** p < 0.002, *** p < 0.0002, and **** p < 0.0001. NC negative control, GM growth medium, OM osteogenic medium.

Article Snippet: After 24 h, the media was changed with osteogenic media (Alfa-MEM with 1% penicillin-streptomycin, 5% fetal bovine serum, 50 μM L-ascorbic acid, 10 mM β-glycerophosphate, and 100 nM dexamethasone) in presence or absence of Thiamet-G (S7213, Selleckchem.com ) with various concentrations.

Techniques: MTS Assay, ALP Activity Assay, Activity Assay, Control, Expressing, Negative Control

Journal: Molecular Pain

Article Title: Non-pain-related CRF1 activation in the amygdala facilitates synaptic transmission and pain responses

doi: 10.1186/1744-8069-9-2

Figure Lengend Snippet: HPA axis function and placement controls. ( A-C ) To suppress HPA axis function rats were pretreated with dexamethasone (Dex, 0.05 mg/kg, subcutaneously) 2 hours prior to CRF administration into the CeLC [ , ]. Injection of Dex had no significant (ns) effect on audible and ultrasonic vocalizations and spinal reflex thresholds measured 15 min before CRF administration (n = 6 rats). Administration of CRF (1 μM, concentration in the microdialysis probe; 15 min) into the CeLC following pretreatment with Dex had significant facilitatory effects (n = 5 rats). ( D-F ) Administration of CRF (1 μM, concentration in the microdialysis probe; 15 min) into the adjacent striatum as a placement control had no significant effect on vocalizations and reflex thresholds (n = 6). Bar histograms show means ± SEM. *,**,*** P < 0.05-0.001, compared to Dex alone; ns (not significant) P > 0.05, compared to predrug; Bonferroni posttests.

Article Snippet: Cyclo(31–34) [D-Phe11,His12,CαMeLeu13,39, Nle17, Glu31, Lys34] Ac-Sauvagine(8–40) (astressin-2B; CRF2 receptor antagonist) [ , ] was purchased from Sigma-Aldrich. (9 R ,10 S ,12 S )-2,3,9,10,11,12-hexahydro-10-hydroxy-9-methyl-1-oxo-9,12-epoxy-1 H -diindolo[1,2,3- fg :3’,2’,1’- kl pyrrolo[3,4- i [ , ]benzodiazocine-10-carboxylic acid, hexyl ester (KT5720; membrane-permeable potent and selective PKA inhibitor) [ ]; 2-[1-(3-dimethylaminopropyl)indol-3-yl]-3-(indol-3-yl) maleimide (GF109203x; membrane-permeable potent and selective PKC inhibitor) [ ]; 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX; non-NMDA receptor antagonist); DL-2-amino-5-phosphonopentanoic acid (AP5; NMDA receptor antagonist); R -( R *, S *)]-6-(5,6,7,8-tetrahydro-6-methyl-1,3-dioxolo[4,5-g]isoquinolin-5-yl)furo[3,4-e]-1,3-benzodioxol-8(6H)-one (bicuculline; GABA A receptor antagonist); and dexamethasone (to suppress HPA axis function [ ]) were purchased from Tocris Bioscience.

Techniques: Injection, Concentration Assay, Control

Journal: Stem cells international

Article Title: The Effects of Platelet-Derived Growth Factor-BB on Bone Marrow Stromal Cell-Mediated Vascularized Bone Regeneration.

doi: 10.1155/2018/3272098

Figure Lengend Snippet: Figure 2: PDGF-BB enhanced BMSC osteogenic differentiation via ERK1/2 signaling pathways. (a) mRNA expression levels of Col-I, OPN, and OCN on days 3 and 7. (b) Protein expression levels of p-ERK, ERK, Col-I, OPN, and OCN on days 3 and 7. (c) ALP staining and semiquantitative analysis of ALP activity. (d) ARS staining and semiquantitative analysis of ARS staining. ALP: alkaline phosphatase; ARS: alizarin red S. Scale bar: 100 μm; ∗P < 0 05; ∗∗P < 0 01.

Article Snippet: For osteogenic differentiation, cells were seeded in 12-well plates and cultured in osteogenic medium (DMEM, 10% FBS, 1% penicillin/ streptomycin, 50μg/mL L-ascorbic acid, 10mM glycerophosphate, and 100 nM dexamethasone) with or without PD98059 (10μM), an extracellular signal-related kinase (ERK) inhibitor (Cell Signaling Technology, Inc.).

Techniques: Protein-Protein interactions, Expressing, Staining, Activity Assay