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Image Search Results
Journal: Annals of Neurosciences
Article Title: ELISA based quantification of Pax6 expression in the developing Zebrafish embryos
doi: 10.5214/ans.0972.7531.220307
Figure Lengend Snippet: (a) ELISA of Pax6 eluted using affinity chromatography (Heparin agarose) in the 8 hpf zebrafish embryos (100 embryos/mL) with high immunoreactivity in the first elution and it was used for further purification. All the values presented are the means ± SD of triplicate assays. (b) ELISA of Pax6 eluted using DEAE cellulose fractions in the 8 hpf zebrafish embryos with high immunoreactivity in Fraction D3 and it was used for further purification. All the values presented are the means ± SD of triplicate assays. (c) ELISA of Pax6 eluted using Sephadex G-75 purification in the 8 hpf zebrafish embryos with high immunoreactivity in Elution G6 and it was used for further assays. All the values are presented are the means ± SD of triplicate assays.
Article Snippet: 1 mL of the supernatant was applied to a column (3 x 9 cm) of
Techniques: Enzyme-linked Immunosorbent Assay, Affinity Chromatography, Purification