ddpcr workflow Search Results


filtration ddpcr workflow heat inactivated sars cov 2  (ATCC)
96
ATCC filtration ddpcr workflow heat inactivated sars cov 2
Filtration Ddpcr Workflow Heat Inactivated Sars Cov 2, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddpcr+workflow/Heat-inactivated+SARS-CoV-2/10__1039_slash_d4ew00526k-77-24-31
Average 96 stars, based on 1 article reviews
filtration ddpcr workflow heat inactivated sars cov 2 - by Bioz Stars, 2026-09
96/100 stars
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qx200 droplet digital pcr system workflow  (Bio-Rad)
96
Bio-Rad qx200 droplet digital pcr system workflow
Cytokine mRNA and protein expression profile following infection of K18-ACE2 mice with Wuhan strain. Infected or mock mouse lung tissues were collected three days post-infection ( n = 4/group). ( A ) The SARS-CoV-2 E gene copy number was evaluated by <t>ddPCR</t> using lung RNA and expressed as number per copy of Rpp30 mRNA (mean ± SD). ( B ) Infectious viral titers were determined in lung homogenates and expressed in TCID 50/mL (mean ± SD). ( C ) Gene expression was evaluated by RT-qPCR and cytokine concentration in lung homogenates determined using a 13-plex Luminex panel. Cytokine gene expression and concentration levels are presented as heatmaps with results expressed as fold (log 2 ) relative to mock-infected mice (mean). Statistical analyses were performed by comparing 2 (−ΔCt) values for each gene in the control group and infected groups with a nonparametric T-test. ( D ) Cytokine concentrations in lung homogenates. Results are expressed as mean ± SD. For protein quantification, statistical analyses were performed by comparing the normalized concentration for each cytokine in the control group and infected groups with a nonparametric t -test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Qx200 Droplet Digital Pcr System Workflow, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ddpcr+workflow/QX200+Droplet+Generator/pmc09964531-112-21-27
Average 96 stars, based on 1 article reviews
qx200 droplet digital pcr system workflow - by Bioz Stars, 2026-09
96/100 stars
  Buy from Supplier

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Cytokine mRNA and protein expression profile following infection of K18-ACE2 mice with Wuhan strain. Infected or mock mouse lung tissues were collected three days post-infection ( n = 4/group). ( A ) The SARS-CoV-2 E gene copy number was evaluated by ddPCR using lung RNA and expressed as number per copy of Rpp30 mRNA (mean ± SD). ( B ) Infectious viral titers were determined in lung homogenates and expressed in TCID 50/mL (mean ± SD). ( C ) Gene expression was evaluated by RT-qPCR and cytokine concentration in lung homogenates determined using a 13-plex Luminex panel. Cytokine gene expression and concentration levels are presented as heatmaps with results expressed as fold (log 2 ) relative to mock-infected mice (mean). Statistical analyses were performed by comparing 2 (−ΔCt) values for each gene in the control group and infected groups with a nonparametric T-test. ( D ) Cytokine concentrations in lung homogenates. Results are expressed as mean ± SD. For protein quantification, statistical analyses were performed by comparing the normalized concentration for each cytokine in the control group and infected groups with a nonparametric t -test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: Viruses

Article Title: SARS-CoV-2 Nsp2 Contributes to Inflammation by Activating NF-κB

doi: 10.3390/v15020334

Figure Lengend Snippet: Cytokine mRNA and protein expression profile following infection of K18-ACE2 mice with Wuhan strain. Infected or mock mouse lung tissues were collected three days post-infection ( n = 4/group). ( A ) The SARS-CoV-2 E gene copy number was evaluated by ddPCR using lung RNA and expressed as number per copy of Rpp30 mRNA (mean ± SD). ( B ) Infectious viral titers were determined in lung homogenates and expressed in TCID 50/mL (mean ± SD). ( C ) Gene expression was evaluated by RT-qPCR and cytokine concentration in lung homogenates determined using a 13-plex Luminex panel. Cytokine gene expression and concentration levels are presented as heatmaps with results expressed as fold (log 2 ) relative to mock-infected mice (mean). Statistical analyses were performed by comparing 2 (−ΔCt) values for each gene in the control group and infected groups with a nonparametric T-test. ( D ) Cytokine concentrations in lung homogenates. Results are expressed as mean ± SD. For protein quantification, statistical analyses were performed by comparing the normalized concentration for each cytokine in the control group and infected groups with a nonparametric t -test. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: SARS-CoV-2 viral RNA loads were determined using Droplet Digital PCR (ddPCR) supermix for probes without dUTP (Bio-Rad Laboratories Ltd.) and the QX200 Droplet Digital PCR System Workflow (Bio-Rad Laboratories Ltd.).

Techniques: Expressing, Infection, Gene Expression, Quantitative RT-PCR, Concentration Assay, Luminex, Control