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tiangen biotech co
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Danaher Inc
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Revvity
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Revvity
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Revvity
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Proteintech
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Danaher Inc
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Revvity
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Danaher Inc
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New England Biolabs
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Image Search Results
Journal: Frontiers in Oncology
Article Title: Spatial transcriptome and single-cell sequencing reveal the role of nucleotide metabolism in breast cancer progression and tumor microenvironment
doi: 10.3389/fonc.2025.1703778
Figure Lengend Snippet: Predicting therapy response with NMRS and validation of the core genes. (A) Estimated IC50 values of Tamoxifen and Palbociclib in high- versus low NMRS groups. (B) Estimated IC50 values of Olaparib and Gefitinib in high- versus low NMRS groups. (C, D) The correlation and differential analysis of drug sensitivity for potential drugs screened from the CTRP and PRISM datasets. (E) Validation of the expression of TAGLN2, PCMT1, PTMA, TUBA3D, and DCTPP1 in a normal breast cell line (MCF-10A) and three BC cell lines (MDA-MB-231, MCF7, and SK-BR3) by qRT-PCR. (F) Immunohistochemical images showing the protein expression of the five genes in the HPA database. **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, ns p > 0.05. BC breast cancer, HPA Human Protein Atlas.
Article Snippet: The antibodies used in this study include:
Techniques: Biomarker Discovery, Expressing, Quantitative RT-PCR, Immunohistochemical staining
Journal: Frontiers in Oncology
Article Title: Spatial transcriptome and single-cell sequencing reveal the role of nucleotide metabolism in breast cancer progression and tumor microenvironment
doi: 10.3389/fonc.2025.1703778
Figure Lengend Snippet: DCTPP1 expression and functional validation in BC (A) DCTPP1 mRNA expression was higher in TCGA-BRCA tumor tissues than in normal tissues ( p < 0.01). (B) Overall survival was worse in TCGA-BRCA patients with high DCTPP1 expression ( p < 0.0001). (C) ROC curve analysis of DCTPP1, highlighting its diagnostic potential. (D) Single-cell analysis showed ( GSE161529 ) that DCTPP1 expression was significantly higher in NUhighepi cells than in others. (E, F) WB and qpcr analysis of the knockdown efficiency of DCTPP1 in MDA-MB-231 (E) and SK-BR-3 (F) cells and showed that the DCTPP1 gene was successfully knocked down in MDA-MB-231 and SK-BR-3 cell lines. (G) CCK-8 showed that the proliferation activity of the cells that knockdown DCTPP1 was dramatically reduced. (H) After DCTPP1 knockdown, the cloning ability of MDA-MB-231 and SK-BR-3 cell lines decreased significantly. (I) Healing test. After DCTPP1 knockdown, the migration ability of MDA-MB-231 and SK-BR-3 cell lines decreased significantly. (J) Transwell assay. After DCTPP1 knockdown, the migration and invasion abilities of MDA-MB-231 and SK-BR-3 cell lines were significantly decreased. **** p < 0.0001, *** p < 0.001, * p < 0.05, ns p > 0.05.
Article Snippet: The antibodies used in this study include:
Techniques: Expressing, Functional Assay, Biomarker Discovery, Diagnostic Assay, Single-cell Analysis, Knockdown, CCK-8 Assay, Activity Assay, Cloning, Migration, Transwell Assay