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Image Search Results
Journal: bioRxiv
Article Title: Circulatory proteins shape microglia state and boost phagocytosis
doi: 10.1101/2024.09.30.615861
Figure Lengend Snippet: Representative flow cytometry data of pre-gated microglia (CD45 low CD11b + CD206 - ) in the hypothalamus region of NHS-Atto647N labeled individual protein-injected mouse comparing to a saline-injected mouse (3-month-old). The percentages of protein-positive microglia are shown. A) Experimental scheme for testing the uptake of NHS-Atto647N labeled individual protein in microglia by flow cytometry. B) Mice injected with Clusterin or Fibrinogen are compared to the saline-injected group. C) Mice injected with labeled ApoM, ApoE or Albumin are compared to the saline-injected group. D) Mice injected with Vitronectin or Fibronectin are compared to the saline-injected group. E) Mice injected with Plasminogen or Vitamin D-binding protein (DBP) are compared to the saline-injected group. F) Mouse injected with Transferrin is compared to the saline-injected group. G) Mouse injected with ApoA-II is compared to the saline-injected group.
Article Snippet: ApoA-I protein (Athens Research &Technology, 16-16-120101-LEL), ApoA-II (Athens Research & Technology, 16-16-120102), ApoM (LSbio, LS-G14868), ApoE (Sigma, SRP4760), Albumin (Sigma, 126674), Clusterin (Sino Biological, 50485), Fibrinogen (Abcam, ab92791), Vitronectin (Sino Biological, 50585-M08H), Fibronectin (Corning, 354008), Plasminogen (Innovative research, IMSPLG1MG), Vitamin D-binding
Techniques: Flow Cytometry, Labeling, Injection, Saline, Binding Assay
Journal: Molecular Medicine Reports
Article Title: Proteomics of acute heart failure in a rat post-myocardial infarction model
doi: 10.3892/mmr.2017.6820
Figure Lengend Snippet: Dynamic changes in differential protein expression in cardiac tissues among the control, 1st and 14th day groups.
Article Snippet: The membranes were probed overnight at 4°C with rabbit anti-myosin 7 polyclonal antibody (cat. no. 22280-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), rabbit anti-Vitamin D binding
Techniques: Expressing, Control, Binding Assay
Journal: Molecular Medicine Reports
Article Title: Proteomics of acute heart failure in a rat post-myocardial infarction model
doi: 10.3892/mmr.2017.6820
Figure Lengend Snippet: Western blot analysis to validate the alterations in the selected proteins identified by proteomic analysis. (A) Cell lysates were extracted from the left ventricle of untreated (control), 1st day post-MI and 14th day post-MI rats and analyzed by western blotting prior to quantification of (B) Myosin-7, (C) Gelsolin, (D) VDBP and (E) Cav1.3 protein expression. GAPDH was used to normalize expression levels. All experiments were performed at least twice. Data are presented as the mean ± the standard deviation. **P<0.01. VDBP, vitamin D binding protein; Cav1.3, voltage-dependent L-type calcium channel subunit α1D; MI, myocardial infarction; control group, operation with no occlusion (untreated); 1st day group, tissues were taken from rats 1 day following surgical procedures; 14th day group, tissues were taken from rats 14 days following surgical procedures.
Article Snippet: The membranes were probed overnight at 4°C with rabbit anti-myosin 7 polyclonal antibody (cat. no. 22280-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), rabbit anti-Vitamin D binding
Techniques: Western Blot, Control, Expressing, Standard Deviation, Binding Assay
Journal: Molecular Medicine Reports
Article Title: Proteomics of acute heart failure in a rat post-myocardial infarction model
doi: 10.3892/mmr.2017.6820
Figure Lengend Snippet: Immunohistochemistry validations of the alterations in the selected proteins identified by proteomic analysis. Myocardial tissues from untreated (control), 1st day post-MI and 14th day post-MI rats were embedded in conventional paraffin and the expression of Myosin-7, VDBP, Gelsolin and Cav1.3 were detected by immunohistochemistry staining using the appropriate antibodies (magnification, ×400). VDBP, vitamin D binding protein; Cav1.3, voltage-dependent L-type calcium channel subunit α1D; MI, myocardial infarction; control group, operation with no occlusion (untreated); 1st day group, tissues were taken from rats 1 day following surgical procedures; 14th day group, tissues were taken from rats 14 days following surgical procedures.
Article Snippet: The membranes were probed overnight at 4°C with rabbit anti-myosin 7 polyclonal antibody (cat. no. 22280-1-AP; ProteinTech Group, Inc., Chicago, IL, USA), rabbit anti-Vitamin D binding
Techniques: Immunohistochemistry, Control, Expressing, Staining, Binding Assay