Journal: BBA Clinical

Article Title: Mutation of EMG1 causing Bowen–Conradi syndrome results in reduced cell proliferation rates concomitant with G2/M arrest and 18S rRNA processing delay

doi: 10.1016/j.bbacli.2014.05.002

Figure Lengend Snippet: Ribosomal subunit levels at steady-state are normal in BCS patient cells. (A, B) Sucrose gradient separation of 40S and 60S ribosomal subunits in lymphoblasts (A) and fibroblasts (B). Ribosomal subunits were detected following sucrose gradient separation by absorbance at 254 nm, and examples of the resultant tracings are shown. (C, D) Fractions were collected at regular intervals and probed by immunoblot analysis for ribosomal proteins using anti-RPS19 and anti-RPL7 antibodies to verify the separation of the small (RPS) and large (RPL) subunits. (E, F) The area under the curve was determined for individual peaks, and the ratio of large to small subunit was calculated. No significant difference between unaffected control and BCS-affected cells was found in lymphoblasts (E) and fibroblasts (F). The mean of six individual experiments and SEM are shown.

Article Snippet: Primary antibody dilutions were used as follows: RPL7 rabbit polyclonal at 1:5000 (Bethyl laboratories), RPS19 rabbit polyclonal at 1:3000 (Proteintech Group), TP53 2B2.68 mouse monoclonal at 1:2000 (Santa Cruz), p21/WAF1/Cip1 mouse monoclonal at 1:250 (Millipore), and β-actin mouse monoclonal at 1:5000 (Sigma).

Techniques: Western Blot, Control

Journal: Neural Development

Article Title: Axon fasciculation in the developing olfactory nerve

doi: 10.1186/1749-8104-5-20

Figure Lengend Snippet: Primary antibodies

Article Snippet: DBA (Biotinylated) , Vector Laboratories , NA , 1:50.

Techniques: Plasmid Preparation

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types

doi: 10.1073/pnas.1713112114

Figure Lengend Snippet: IgG responses to PADs in DBA/2 mice. Plates were coated with PADs and blocked with BSA. Sera from primed mice were obtained at 15, 35, 55, and 65 d postimmunization and were diluted at 1/40. ( A ) For PBS-immunized mice, nine sera were tested for murine PAD2 and human PAD4. ( B ) For PAD-immunized mice, each serum was tested against the same PAD used for each immunization. After washing, peroxidase-conjugated antimurine IgG was added. The OD was read at 405 nm. The background OD was obtained by adding each serum to a well without PAD (negative). Positive sera were defined as an OD value higher than twice the background OD.

Article Snippet: C3H/HeNHsd and DBA/2 mice were purchased from Envigo Laboratories.

Techniques:

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types

doi: 10.1073/pnas.1713112114

Figure Lengend Snippet: T cell responses to PADs in DBA/2 mice. Spleen and lymph nodes were obtained at 65 d postimmunization. Cells were extracted and cultured at 5 × 10 6 cells per well with 2 μg of proteins or PHA. T cell responses were evaluated by bromodeoxyruridine incorporation. Positive responses were defined as an OD value higher than twice the OD for cells cultured without protein.

Article Snippet: C3H/HeNHsd and DBA/2 mice were purchased from Envigo Laboratories.

Techniques: Cell Culture

Journal: Proceedings of the National Academy of Sciences of the United States of America

Article Title: Peptidyl arginine deiminase immunization induces anticitrullinated protein antibodies in mice with particular MHC types

doi: 10.1073/pnas.1713112114

Figure Lengend Snippet: Citrullinated peptide detection in the sera of DBA/2 mice immunized with PADs or PBS. ELISA plates were coated with 46 citrullinated peptides and blocked with BSA. Sera from primed mice obtained at 55 and 65 d postimmunization were diluted at 1/80. After washing, peroxidase-conjugated antimurine IgG was added. The OD was read at 405 nm. The background OD was obtained by adding each serum to a well without peptide. Positive sera were defined as an OD value higher than twice the background OD. A column corresponded to one mouse.

Article Snippet: C3H/HeNHsd and DBA/2 mice were purchased from Envigo Laboratories.

Techniques: Enzyme-linked Immunosorbent Assay