Journal: Yonsei Medical Journal

Article Title: Methionyl-tRNA Synthetase is a Useful Diagnostic Marker for Lymph Node Metastasis in Non-Small Cell Lung Cancer

doi: 10.3349/ymj.2019.60.11.1005

Figure Lengend Snippet: Immunoblotting of methionyl-tRNA synthetase (MRS)-positive H460 cells, CD45/CD3-positive Molt-4 cells, and CD45/CD20-positive Daudi cells (A). Fluorescent immunochemical staining for MRS and CD45 in the reference samples containing a fixed ratio of H460, Daudi, and Molt-4 cells (B and C) and endobronchial ultrasound-guided transbronchial needle aspirate-derived samples (D and E). Images were taken using a fluorescent microscope (B and D) and confocal microscope (C and E). Blue represents nucleus (4′, 6-Diamidine-2′-phenylindole dihydrochloride: DAPI); green, MRS; and red, CD45. B and D confocal images show merged images only. TTF-1, thyroid transcription factor 1.

Article Snippet: Molt-4, Daudi, and H460 cells were obtained from the Korean Cell Line Bank (Seoul, Korea).

Techniques: Western Blot, Staining, Derivative Assay, Microscopy

Journal: eLife

Article Title: Caspase-mediated cleavage of IRE1 controls apoptotic cell commitment during endoplasmic reticulum stress

doi: 10.7554/eLife.47084

Figure Lengend Snippet:

Article Snippet: Cell line (human) , Daudi , Genentech , RRID: CVCL_0008 , Burkitt’s lymphoma.

Techniques: Knock-Out, Isolation, Clone Assay, CRISPR, Transfection, Construct, FLAG-tag, shRNA, Recombinant, Caspase-Glo Assay, Cell Viability Assay, Flow Cytometry, Fractionation, Cell Culture, Purification, Software

Journal: PLOS ONE

Article Title: Long non-coding RNA SNHG17 may function as a competitive endogenous RNA in diffuse large B-cell lymphoma progression by sponging miR-34a-5p

doi: 10.1371/journal.pone.0294729

Figure Lengend Snippet: (A) Survival analyses of GSE10846 (n = 420) in DLBCL patients with high and low expression of SNHG17.(B, C) Kaplan–Meier survival analysis. (D, E) Uniform and multivariate expression analyses (all bars correspond to the 95% confidence intervals). (F) Nomogram was used to predict the OS of patients. (G) Expression of SNHG17 in DLBCL patients’ tumor and normal tissues.The sequencing data for normal cells and diffuse large B-cell lymphoma were obtained from UCSC XENA, which we have described in the methods section. (H) qPCR shows the expression of SNHG17 in lymphoma cell lines (Daudi, Ramos, DoHH2, Farage, and Raji) and peripheral blood mononuclear cells (PBMC).*P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: PBMC and B lymphoma cell lines (Daudi, Ramos, DoHH2, Farage, and Raji) were purchased from Procell (Nanjing Cobioer Biosciences Co, LTD, China) and cultured in a 1640 Roswell Park Memorial Institute (RPMI-1640) medium containing 10% fetal bovine serum.

Techniques: Expressing, Sequencing

Journal: Immunology

Article Title: The cytotoxic molecule granulysin is capable of inducing either chemotaxis or fugetaxis in dendritic cells depending on maturation: a role for V δ 2 + γδ T cells in the modulation of immune response to tumour?

doi: 10.1111/imm.13248

Figure Lengend Snippet: V δ 2 + γδ T cells release granulysin in response to tumour. (a) The expression of exhaustion markers PD‐1 and Lag‐3 on, and the secretion of granulysin by, V δ 2 + γδ T cells during the 9‐day expansion process. (b) The percentage of V δ 2 + γδ T cells to express early activation marker CD69 following 24, 48 or 72 hr of culture with Daudi cells, Raji cells or Raji cells pre‐treated for 24 hr with 5 μ m zoledronic acid (ZA), as determined by flow cytometry. (c) The concentration of interferon‐ γ (IFN‐ γ ) found within supernatants taken from 24, 48 or 72 hr co‐culture of V δ 2 + γδ T cells with tumour cell lines, as determined by ELISA. (d) The percentage of V δ 2 + γδ T cells to express degranulation marker CD107a following 24, 48 or 72 hr of culture with Daudi cells, Raji cells or Raji cells pre‐treated for 24 hr with 5 μ m ZA, as determined by flow cytometry. (e) The concentration of granulysin found within supernatants taken from 24, 48 or 72 hr co‐culture of V δ 2 + γδ T cells with tumour cell lines, as determined by ELISA. (f) The concentration of granzyme B found within supernatants taken from 24, 48 or 72 hr co‐culture of V δ 2 + γδ T cells with tumour cell lines, as determined by ELISA. (g) Percentage killing of tumour cells by V δ 2 + γδ T cells following 24, 48 and 72 hr of culture, as determined by flow cytometry. Data shown are from six independent experiments, using V δ 2 + γδ T cells from six individual donors, with error bars (SD). Differences between groups were assessed by two‐way analysis of variance comparing negative control (V δ 2 + γδ T cells alone) with all other groups. * P < 0·05. ** P < 0·01. *** P < 0·001. **** P < 0·0001.

Article Snippet: Daudi and Raji B‐cell lymphoma lines (European Collection of Authenticated Cell Cultures, Salisbury, UK) were used in experiments as γδ T‐cell‐susceptible and ‐resistant target cells, respectively.

Techniques: Expressing, Activation Assay, Marker, Flow Cytometry, Concentration Assay, Co-Culture Assay, Enzyme-linked Immunosorbent Assay, Negative Control

Journal: Aging (Albany NY)

Article Title: The improved anticancer effects of Bortezomib-loaded hollow mesoporous silica nanospheres on lymphoma development

doi: 10.18632/aging.202146

Figure Lengend Snippet: BTZ@HMSNs restricts the proliferation of lymphoma. ( A ) Cell viability of SNK-1 cells treated with PBS (control), HMSNs, BTZ (set from 1 to 150 nM), HMSNs+BTZ (containing 1-150 nM of BTZ) and BTZ@HMSNs (containing 1-150 nM of BTZ) by MTT assay. ( B ) The gene expression profile of Daudi, Jurkat, Raji, SNK-1 and NK92 cells by qRT-PCR (Left). Cell viability of indicated cells treated with BTZ or BTZ@HMSNs by MTT assay (Right). ( C ) Colony number of SNK-1 cells treated with PBS (control), HMSNs, BTZ, BTZ@HMSNs by colony formation assay. BTZ, bortezomib; HMSNs, hollow mesoporous silica nanospheres. The differences between groups were analyzed by one-way ANOVA followed by multiple comparison with Tukey test. ** P < 0.01 and *** P < 0.001. Values are means ± SD.

Article Snippet: Human NK cell lymphoma cell line SNK-1 and B cell lymphoma cell lines Raji and Daudi were obtained from Shanghai Obio Technology Co., Ltd (China), and T cell lymphoma cell line Jurkat and NK cell lymphoma cell line NK-92 were purchased from the American Type Culture Collection (ATCC).

Techniques: Control, MTT Assay, Gene Expression, Quantitative RT-PCR, Colony Assay, Comparison