Journal: Communications Biology

Article Title: NUPR1 protects against hyperPARylation-dependent cell death

doi: 10.1038/s42003-022-03705-1

Figure Lengend Snippet: a OXPHOS metabolism, reflected by oxygen consumption rate (OCR) levels were measured in MiaPaCa-2 cells after 24 h treatments. b MiaPaCa-2 cells were treated with ZZW-115 (1.5 µM) and olaparib (25 µM) for 24 h, then, loaded with MitoTracker Deep-Red FM and, after fixation, stained with DAPI. Flow cytometry analysis were carried out using MitoProbe™ TMRM ( c ), MitoSOX™ Red ( d ), CellROX™ Orange Reagent ( e ) or Fluo-4-AM ( f ) for analysis of the mitochondrial membrane potential, mitochondrial superoxide levels, intracellular ROS levels and the cytosolic calcium concentration, respectively, after 24 h of incubation with the drugs. Data represent mean ± SEM. Two-way, Sidak was used, * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.001. g PLA was performed in MiaPaCa-2 cells in the presence or in the absence of ZZW-115 together or not with 5-FU in the presence or absence of olaparib. Mouse anti-PAR and rabbit anti-Mitofusin2 antibodies were used. A representative experiment is shown ( n = 3). ImageJ was used to count the number of green dots. Mean ± SEM of foci/nucleus are included.

Article Snippet: Finally, samples were mounted using the Prolong Gold antifade reagent with DAPI (Thermon Fisher).

Techniques: Staining, Flow Cytometry, Membrane, Concentration Assay, Incubation

Journal: bioRxiv

Article Title: Preserving endothelial integrity in human saphenous veins during preparation for coronary bypass surgery

doi: 10.1101/2023.08.25.554690

Figure Lengend Snippet: Representative images of immunofluorescent detection of endothelial markers (A) VE-cadherin and (B) eNOS expression (n=7). EC markers are shown in pink while DAPI labeled nuclei are shown in blue. Lower magnification images of the whole vessels contain pseudo-color lines indicating staining positive areas in orange and enlarged images contain orange arrows indicating staining positivity. Pink boxes indicate the amplified image area. Graphs represent the percentage of each vessel lumen determined to be positive for endothelial markers.

Article Snippet: Samples were mounted using FadeStop TM fluorescent mounting reagent with DAPI (GenuIN Biotech #272).

Techniques: Expressing, Labeling, Staining, Amplification