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Journal: bioRxiv
Article Title: The Prefrontal Cortex to the Hippocampus Glutamatergic Pathway Regulates Reward Effects of Methamphetamine
doi: 10.1101/2024.05.20.595065
Figure Lengend Snippet: a , Anterograde tracing rAAVs. b , c , The schematic of the anterograde tracing strategy. Diagram of virus injection site in the PrL and optical fiber implantation site in the dCA1. d , The schematic of the experimental timeline of rAAVs expression and METH-CPP test on 12th day (no opto) combining with light stimulation on 13th day (with opto). e , METH-CPP expression in mCherry (n=8 mice) vs. ChR2-mCherry expressing mice (n=9 mice). (two-way ANOVA, interaction, F (2,45) =4.958, P =0.0113; CPP (pre-, post-no, post-with opto), F (1,45) =3.955, P =0.0528; mCherry (n.c, ChR2), F (2,45) =14.95, P <0.0001, Bonferroni’s multiple comparisons test), n.c, negative control group. f , METH-CPP expression in mCherry (n=8 mice) vs. eNpHR3.0-mCherry expressing mice (n=7 mice). (two-way ANOVA, interaction, F (2,39) =3.210, P =0.0512; CPP (pre-, post-no, post-with opto), F (1,39) =7.004, P= 0.0117; mCherry (n.c, eNpHR3.0), F (2,39) =24.71, P= 0.0001, Bonferroni’s multiple comparisons test), n.c, negative control group. g , h , Expression of mCherry and DAPI in the PrL (g) and dCA1 (h) (n = 3 mice), DAPI, 4′,6-diamidino-2-phenylindole, Scale bar, 100 μm, 20 μm. i , The schematic of the experimental timeline of rAAVs expression and METH-CPP test on 12th day (with opto). j , METH-CPP expression in mCherry (n=7 mice) vs. ChR2-mCherry expressing mice (n=8 mice). (two-way ANOVA, interaction, F (1,26) =9.920, P =0.0041; CPP (pre-, post-with opto), F (1,26) =17.21, P =0.0003; mCherry (n.c, ChR2), F (1,26) =14.61, P =0.0007, Bonferroni’s multiple comparisons test), n.c, negative control group. k , METH-CPP expression in mCherry (n=7 mice) vs. eNpHR3.0-mCherry expressing mice (n=6 mice). (two-way ANOVA, interaction, F (1,22) =3.902, P =0.0609; CPP (pre-, post-with opto), F (1,22) =5.981, P =0.0229; mCherry (n.c, eNpHR3.0), F (1,22) =66.58, P < 0.0001, Bonferroni’s multiple comparisons test), n.c, negative control group. l , m , The expression of mCherry and DAPI in the PrL ( l ) and dCA1 ( m ) (n = 3 mice). Scale bar, 100 μm, 20 μm. Data in e , f , j , k are presented as means±s.e.m. Two-sided statistical tests were used. *p <0.05, **p <0.01, ***p <0.001; ns, not significant.
Article Snippet: After rinsing in PBS, the slices were incubated with fluorescence-conjugated secondary antibody at room temperature for 2 h. Finally, slices were coverslipped on anti-fluorescence attenuation tablets including
Techniques: Anterograde Tracing, Virus, Injection, Expressing, Negative Control