Journal: Nuclear medicine and biology

Article Title: Initial insights into the interaction of antibodies radiolabeled with Lutetium-177 and Actinium-225 with tumor microenvironment in experimental human and canine osteosarcoma.

doi: 10.1016/j.nucmedbio.2024.108917

Figure Lengend Snippet: Fig. 5. Quantitative analysis and representative IHC images of M1 macrophages in human OS-33 tumors and canine Gracie tumors obtained from the OS tumors bearing mice treated with [177Lu]Lu-IF3 or [225Ac]Ac-IF3 radioconjugates. Tumor slides were stained with Anti-CD11c (D1V9Y) (97585) rabbit IgG mAb. (A-D) M1 macrophages in human OS-33 tumors and canine Gracie tumors obtained from the OS tumors bearing mice treated with [177Lu]Lu-IF3 or [225Ac]Ac-IF3 and har vested at different time points (control: black; 24 h: pink; 72 h: green; 168 h: purple) (A) canine Gracie tumors treated with [177Lu]Lu-IF3. (B) human OS-33 tumors treated with [177Lu]Lu-IF3. (C) canine Gracie tumors treated with [225Ac]Ac-IF3. (D) human OS-33 tumors treated with [225Ac]Ac-IF3. * p < 0.05.

Article Snippet: Rabbit IgG anti-CD11c (D1V9Y) (#97585) was purchased from Cell Signaling (Danvers, Massachusetts, USA).

Techniques: Staining, Control

Journal: Small (Weinheim an Der Bergstrasse, Germany)

Article Title: Filomicelle‐Embedded Composite Hydrogels for Localized Gelation Within the Anterior Chamber of the Eye

doi: 10.1002/smll.202508236

Figure Lengend Snippet: Biocompatibility of intracamerally injected C‐gel. a,c) Optical microscopic images and b,d) high‐resolution anterior segment OCT images of mouse eyes on day 7 after intracameral injection. (a,b) Eye injected with 0.6 µL of C‐gel. (c,d) Eye injected with 0.6 µL of saline. Opaque C‐gel was clearly visible throughout the anterior chamber. No signs of corneal edema, endothelial disruption, anterior chamber shallowing, iris abnormalities, or aqueous turbidity were detected. e,g) Optical microscopic images and f,h) high‐resolution anterior segment OCT images of mouse eyes at week 5 post‐intracameral injection. C‐gel is indicated by yellow dotted lines. (e,f) Eye injected with 0.6 µL of C‐gel. (g,h) eye injected with 0.6 µL saline. A decrease in C‐gel opacity in the optical image and reduced contrast in the OCT image were observed at week 5. i) Representative H&E‐stained images of the peripheral and central cornea from eyes received C‐gel (left) and saline (right). EP, corneal epithelium; S, stroma; EN, corneal endothelium. Scale bar is 50 µm. j) Representative immunofluorescence images of the peripheral and central cornea from eyes that received C‐gel (left) or saline (right). Sections were stained for CD68 (green) and CD11c (red). Scale bar is 10 µm.

Article Snippet: For immunofluorescence staining, tissue blocks were sectioned at 4 μm thickness and stained with rabbit monoclonal antibodies against CD11c (D1V9Y) and CD68 (E3O7V) (Cell Signaling Technology).

Techniques: Injection, Saline, Disruption, Staining, Immunofluorescence