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Lonza
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Merck KGaA
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Image Search Results
Journal: Biomedicines
Article Title: Mitochondrial Respiration of Platelets: Comparison of Isolation Methods
doi: 10.3390/biomedicines9121859
Figure Lengend Snippet: Scheme of platelets (PLT) preparation using density gradient centrifugation (DC). From the first centrifugation of whole blood with Ficoll-Paque TM to the final pellet, which was resuspended in Dulbecco’s phosphate-buffered solution (DPBS) and ethylene glycol-bis (2-aminoethyl ether)- N , N , N ′, N ′-tetra-acetic acid (EGTA) at room temperature (RT). The PLT suspension was counted (10 times diluted) and added to the O2k-chamber at a final cell concentration of 200 × 10 6 x·mL −1 . PBMC, peripheral blood mononuclear cell.
Article Snippet: Calcium-free Dulbecco’s phosphate-buffered
Techniques: Gradient Centrifugation, Centrifugation, Concentration Assay