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Image Search Results
Journal: Stem Cells International
Article Title: YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma
doi: 10.1155/2022/7494354
Figure Lengend Snippet: YTHDC1 aberrantly expressed in HNSCC and was associated with cancer cell stemness. (a) The relative mRNA expression of YTHDC1 in tumor and normal tissues in HNSCC in TCGA database. The comparison of the YTHDC1 mRNA expression in (b) individual tumor grades, (c) HPV infection status, and (d) different nodal metastasis status. (e) The Pearson correlation between YTHDC1 and SOX2 mRNA expression in HNSCC according to TCGA data. (f) The Pearson correlation between YTHDC1 and BMI1 mRNA expression in HNSCC according to the TCGA data.
Article Snippet: The following antibodies were used in the experiment: GAPDH (Proteintech, 10494-1-AP),
Techniques: Expressing, Comparison, Infection
Journal: Stem Cells International
Article Title: YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma
doi: 10.1155/2022/7494354
Figure Lengend Snippet: YTHDC1 was overexpressed in HNSCC patients. (a) Representative graph of YTHDC1 staining in tumor and normal tissues of HNSCC patients (left) and the IHC score quantification (right) ( n = 6). Scale bar, 100 μ m. (b) The YTHDC1 expression in patients HNSCC tissues and normal tissues detected by immunoblotting analysis ( n = 3). (c) The SOX2 and BMI1 protein expression in patient HNSCC tissues and normal tissues detected by immunoblotting analysis ( n = 3).
Article Snippet: The following antibodies were used in the experiment: GAPDH (Proteintech, 10494-1-AP),
Techniques: Staining, Expressing, Western Blot
Journal: Stem Cells International
Article Title: YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma
doi: 10.1155/2022/7494354
Figure Lengend Snippet: Inhibition of the YTHDC1 expression significantly limits tumor cell stemness maintenance, migration, and proliferation. (a) Western blotting of YTHDC1 in six HNSCC cell lines. (b) Western blotting of YTHDC1 in SCC9 and HN4 with or without YTHDC1 knockdown. (c) qPCR data of YTHDC1 in SCC9 and HN4 with or without YTHDC1 knockdown. (d) SOX2 and BMI1 protein expression in SCC9 and HN4 with or without YTHDC1 knockdown. (e) The OCT4 and NANOG protein expression in SCC9 and HN4 with or without YTHDC1 knockdown. (f). SCC9 and HN4 cell proliferation ability was suppressed after the YTHDC1 expression was decreased. (g) Sphere formation ability of SCC9 and HN4 cell lines was inhibited after the YTHDC1 expression was suppressed. (h) Representative images and quantification of migration assay of SCC9 and HN4 cell lines with or without YTHDC1 knockdown. (i) Representative images and quantification of colony formation assay of SCC9 and HN4 cell lines with or without YTHDC1 knockdown. (j). Tumor xenograft experiment in nude mice detects the effect of YTHDC1 on tumor growth in vivo ( n = 6 in each group). (k) Tumor growth curve. The quantitative value of tumor volumes is plotted, and tumor volumes are measured once two days after injected. (l) Scatter diagram shows tumor weight.
Article Snippet: The following antibodies were used in the experiment: GAPDH (Proteintech, 10494-1-AP),
Techniques: Inhibition, Expressing, Migration, Western Blot, Knockdown, Colony Assay, In Vivo, Injection
Journal: Stem Cells International
Article Title: YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma
doi: 10.1155/2022/7494354
Figure Lengend Snippet: Validation of the role of YTHDC1 in regulating stemness maintenance at the single cell level. (a) The UMAP plot of scRNA-seq cell data labeled by cell type. (b) The UMAP plot of the YTHDC1 expression in all the clusters. (c) The UMAP plot of cancer cells from HNSCC tissues shows the YTHDC1 expression pattern. (d) The Pearson correlation between YTHDC1 and SOX2 mRNA expression according to the single cell RNA data. (e) The Pearson correlation between YTHDC1 and BMI1 mRNA expression according to the single cell RNA data. (f) Volcano plot shows differentially expressed genes between YTHDC1 high and YTHDC1 low cells. (g) GO analysis of the upregulated genes in the YTHDC1 high expression cell population. (h) KEGG analysis of the upregulated genes in the YTHDC1 high expression cell population. (i) Violin plot depicts the expression of stem cell-related markers of YTHDC1 high and YTHDC1 low cells.
Article Snippet: The following antibodies were used in the experiment: GAPDH (Proteintech, 10494-1-AP),
Techniques: Biomarker Discovery, Labeling, Expressing