Journal: Biomolecules

Article Title: The Ensemble of Conformations of Antifreeze Glycoproteins (AFGP8): A Study Using Nuclear Magnetic Resonance Spectroscopy

doi: 10.3390/biom9060235

Figure Lengend Snippet: Sequence-specific chemical shift assignments of antifreeze glycoprotein fraction 8 (AFGP8). The 2D TOCSY NMR spectra of AFGP8-BS (AFGP8 from Boreogadus saida ) ( a ) and AFGP8-TB (AFGP8 from Trematomus borchgrevinki ) ( b ), showing the amide to alpha and methyl proton regions. The TOCSY patterns associated with all the amino acid residues of the primary sequence, except for the alanine residue at position 1 and proline residues at positions 4 and 10 in AFGP8-BS and positions 7 and 13 in AFGP8-TB.

Article Snippet: The disaccharide beta- d -galactosyl-(1,3)-alpha-N-acetyl- d -galactosamine (5 mg, 98% purity, CAS 3554-90-3, Cat No. A152000) was purchased from Toronto Research Chemicals (North York, ON, Canada) and was used as purchased.

Techniques: Sequencing, Residue

Journal: Process Biochemistry

Article Title: A novel cold-active β-d-galactosidase with transglycosylation activity from the Antarctic Arthrobacter sp. 32cB – Gene cloning, purification and characterization

doi: 10.1016/j.procbio.2014.09.018

Figure Lengend Snippet: Fig. 7. TLC of products of alkyl glycosides synthesis catalyzed by Arthrobacter sp. 32cB -d-galactosidase. Reaction mixtures containing lactose and 1-butanol (A), 2-propanol (B), 1-hexanol (C) or cyclohexanol (D) as substrates, and 2 U mL−1 Arthrobacter sp. 32cB -d-galactosidase were incubated at 30 ◦C for 8 h. Lane 1 – d-galactose, lane 2 – d-glucose,

Article Snippet: Materials Isopropyl- -d-thiogalactopyranoside (IPTG) and 5-bromo4-chloro-3-indolyl- -d-galactopyranoside (X-gal) were purchased from Biosynth AG (Switzerland). o-Nitrophenyl-d-galactopyranoside (ONPG), p-nitrophenyl- -d-galactopyranoside (PNPG), p-nitrophenyl- -d-glucopyranoside, p-nitrophenyl- -d-fucopyranoside, p-nitrophenyl- -d-xylopyranoside, p-nitrophenyl- -d-glucouronide, p-nitrophenyl- -d-manno pyranoside, p-nitrophenyl- -d-cellobioside, p-nitrophenyl- - l-arabinopyranoside, p-nitrophenyl- -d-galactopyranoside, p-nitrophenyl- -d-glucopyranoside, l-arabinose, d-xylose, lactulose, dithiothreitol (DTT), ethylenediaminetetraacetic acid sodium salt (EDTA), N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES) and tris(2-carboxyethyl)phosphine (TCEP) were supplied by Sigma (USA).

Techniques: Incubation