Journal: bioRxiv

Article Title: The phagosome-mediated anti-bacterial immunity is governed by the proton-activated chloride channel in peritoneal macrophages

doi: 10.1101/2025.02.27.640612

Figure Lengend Snippet: A, Volcano plot depicting differentially secreted proteins in Cx3cr1 -PAC −/− (left) or LysM -PAC −/− (right) peritoneal fluids after 4 hours of peritoneal infection. Highlighted proteins represent top differentially secreted proteins with p < 0.05 and average log-transformed fold-change > 0.25. B , Quantification of highlighted proteins in ( A ), excluding proteins with absolute concentration below 100 pg/mL. Each genotype has n = 6 mice in independent experiments. C , UMAP color-coded for immune cell types based on the expression of cell-type enriched surface markers before and after infection. NK, natural killer cells. DCs, dendritic cells. D , FlowSOM analyzed colored tree for immune cell types based on the expression of cell-type enriched surface markers post-infection in control (left) or LysM -PAC −/− (right) peritoneal fluids. E , Donut plots depicting composition of peritoneal immune cell types with respect to genotypes and treatments quantified with manual gating. F , Manual gating strategy of indicated immune cell types in cyTOF dataset. DN, double negative. G , Quantification of neutrophil and Ly6C high monocyte populations through manual gating strategy. Each condition and genotype have at least n > 3 mice in independent experiments. All control groups represent Pacc1 F/F genotype. Data are reported as mean ± SEM. Unpaired t-test for B . Two-way ANOVA with Sidak’s test for G . *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Article Snippet: cyTOF FCS files acquired and exported from the Johns Hopkins SKCCC Flow/Mass Cytometry Core were uploaded to OMIQ.

Techniques: Infection, Transformation Assay, Concentration Assay, Expressing, Control