Journal: Cell Death & Disease

Article Title: ISGylation prevents autophagic degradation of STING and promotes antitumor immunity in lung cancer

doi: 10.1038/s41419-026-08527-1

Figure Lengend Snippet: A The inhibitory activity of TST against USP18 was measured using the peptide RLRGG-AMC as a substrate. IC 50 was presented as mean ± SEM, n = 3 independent experiments. The chemical structure of TST is shown. B STD-NMR binding analysis for TST and USP18. The top spectrum is the 1 H NMR of USP18 (10 μM) in a mixture with TST (400 μM), while the bottom spectrum displays the STD-NMR of the same sample. C USP18-mediated de-ISGylation of STING examined by Western blot. Flag-STING, UBE1L-Myc-His (E1), UBCH8-Myc-His (E2), HERC5-Myc-His (E3), and Myc-ISG15 were co-transfected with GFP-USP18-WT into HEK-293T cells. Twenty-four hours post-transfection, cells were treated with TST (5, 25,125 μM) for 12 h, followed by an ISGylation assay. ISGylation experiments were performed as described in (Fig. ). D CHX chase assay was performed to analyze the effect of TST on STING protein stability in both USP18-WT (Negative Control, si-NC) and USP18-KD cells. LLC cells were treated with 100 µg/mL cycloheximide (CHX) for the indicated time points. The graph represents quantification of the STING protein levels. E CHX chase assay was used to analyze the effect of TST on STING protein stability in cells expressing either STING-WT or STING-4KR. The LLC cells were treated with 100 µg/mL cycloheximide (CHX) for the indicated time points. The graph represents quantification of the STING protein levels. F–G The synergistic antitumor effect of combined treatment of TST (10 mg/kg) and diABZi (3 mg/kg) in a mouse model. Tumors were dissected, photographed F and weighed G . H–K Tumor-infiltrating CD8 + T cells and NK1.1 + cells among CD45 + cells were analyzed using flow cytometry. Representative scatter plots H, J and frequencies I, K are shown ( n = 5 per group). The data presented is from a representative experiment, selected from three independent experiments. Data were presented as mean ± SEM, comparisons were conducted using two-way ANOVA for multiple comparisons, with ** indicating p < 0.01, *** indicating p < 0.001, **** indicating p < 0.0001 and n.s indicating not significant.

Article Snippet: Cells were treated with TST (50 μM) for 12 h and then 50 μg/mL of CHX (T1225, Topscience) at specific time intervals.

Techniques: Activity Assay, Binding Assay, Western Blot, Transfection, Negative Control, Expressing, Flow Cytometry

Journal: bioRxiv

Article Title: Identification and SAR optimization of FBXO22-mediated TEAD Targeted Glue™ degraders

doi: 10.64898/2026.04.21.719895

Figure Lengend Snippet: Immunoblot and quantification of TEAD protein levels in NCI-H226 following DMSO or 10 µg/ml cycloheximide over a time course. Data are from n = 1 biologically independent samples.

Article Snippet: MLN4924 (TOCRIS, #6499, dissolved to 10 mM in DMSO) stock was used for final concentration 3 μM; cycloheximide (ThermoFisher Scientific, #J66004.XF, diluted to 10 mg/ml in media) was used for final concentration 10 μg/ml; AG (Sigma-Aldrich, #396494) was solubilised to 15 mM in complete medium, made fresh for each experiment.

Techniques: Western Blot