Journal: American Journal of Translational Research

Article Title: Curcumin inhibits gastric cancer-derived mesenchymal stem cells mediated angiogenesis by regulating NF-κB/VEGF signaling

doi:

Figure Lengend Snippet: NF-κB/VEGF signaling regulated GC-MSC-induced tube formation, migration and colony formation in HUVEC cells. A. Bar graphs showing quantifications of tube formation in HUVEC cells cultured in conditioned media from GC-MSCs treated with either NF-κB inhibitor PDTC (20 μM) or VEGF-specific blocking antibody or no treatment or treated with control antibody (**P < 0.01). B. Transwell Migration Assay showing the migration ability of HUVECs cultured in conditioned media from GC-MSCs treated either with NF-κB inhibitor, PDTC or VEGF-specific blocking antibody (**P < 0.01). C. Cell colony formation assay showing the effect of NF-κB inhibitor, PDTC and VEGF-specific blocking antibody on HUVEC proliferation GC-MSC-induced (*P < 0.05). D. Western blot analysis of the effects of NF-κB inhibitor PDTC and VEGF-specific blocking antibody on PCNA, cyclinD/cyclinE and antiapoptotic proteins BCL-XL, BCL-2 in HUVECs.

Article Snippet: The following primary antibodies were used: α-SMA, vimentin, (Abcam, USA); IκBα, t-NF-κB p65, p-NF-κB p65 (Cell Signaling Technology, Beverly, MA, USA); VEGF (Santa Cruz Biotechnology, Inc., USA); cyclinD, cyclinE, proliferating cell nuclear antigen (PCNA), BCL-2, and BCL-XL (Bioworld Technology, Louis Park, MN, USA).

Techniques: Migration, Cell Culture, Blocking Assay, Transwell Migration Assay, Colony Assay, Western Blot