cycler Search Results


cd45r b220 antibody anti mu cd45r b220 akyp0014 bx010 alexa fluortm 750 for phenocycler formerly cd45r b220 bx010 ra3 6b2  (Akoya Biosciences)
97
Akoya Biosciences cd45r b220 antibody anti mu cd45r b220 akyp0014 bx010 alexa fluortm 750 for phenocycler formerly cd45r b220 bx010 ra3 6b2
Cd45r B220 Antibody Anti Mu Cd45r B220 Akyp0014 Bx010 Alexa Fluortm 750 For Phenocycler Formerly Cd45r B220 Bx010 Ra3 6b2, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/PhenoCycler/pm41193846-1261-7-6
Average 97 stars, based on 1 article reviews
cd45r b220 antibody anti mu cd45r b220 akyp0014 bx010 alexa fluortm 750 for phenocycler formerly cd45r b220 bx010 ra3 6b2 - by Bioz Stars, 2026-09
97/100 stars
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normobaric hypoxia chambers  (BioSpherix)
96
BioSpherix normobaric hypoxia chambers
Normobaric Hypoxia Chambers, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/OxyCycler+A84XOV/pm41896623-251-6-14
Average 96 stars, based on 1 article reviews
normobaric hypoxia chambers - by Bioz Stars, 2026-09
96/100 stars
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cd8 bx026  (Akoya Biosciences)
97
Akoya Biosciences cd8 bx026
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Cd8 Bx026, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/PhenoCycler+Antibody+Database/pmc10983045-4-0-4
Average 97 stars, based on 1 article reviews
cd8 bx026 - by Bioz Stars, 2026-09
97/100 stars
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alimentarius procedural  (Akoya Biosciences)
99
Akoya Biosciences alimentarius procedural
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Alimentarius Procedural, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/PhenoCycler-Fusion+2%2E0/10__1002_slash_jssc__201401262-222-12-11
Average 99 stars, based on 1 article reviews
alimentarius procedural - by Bioz Stars, 2026-09
99/100 stars
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c1000 touch thermal cycler  (Bio-Rad)
97
Bio-Rad c1000 touch thermal cycler
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
C1000 Touch Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/C1000+Touch+Thermal+Cycler+with+96%E2%80%93Deep+Well+Reaction+Module/bio_rxiv__2025__06__04__657887-133-34-38
Average 97 stars, based on 1 article reviews
c1000 touch thermal cycler - by Bioz Stars, 2026-09
97/100 stars
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c1000 touch thermocycler  (Bio-Rad)
96
Bio-Rad c1000 touch thermocycler
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
C1000 Touch Thermocycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/C1000+Touch+Thermal+Cycler+with+96-Well+Fast+Reaction+Module/pm41525168-74-14-17
Average 96 stars, based on 1 article reviews
c1000 touch thermocycler - by Bioz Stars, 2026-09
96/100 stars
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biorad t100 thermal cycler  (Bio-Rad)
99
Bio-Rad biorad t100 thermal cycler
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Biorad T100 Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/T100+Thermal+Cycler/pm42107245-74-17-17
Average 99 stars, based on 1 article reviews
biorad t100 thermal cycler - by Bioz Stars, 2026-09
99/100 stars
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c1000 touch thermal cycler with dual 48 48 fast reaction module  (Bio-Rad)
98
Bio-Rad c1000 touch thermal cycler with dual 48 48 fast reaction module
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
C1000 Touch Thermal Cycler With Dual 48 48 Fast Reaction Module, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/C1000+Touch+Thermal+Cycler+with+Dual+48%2F48+Fast+Reaction+Module/pmc12859244-139-0-11
Average 98 stars, based on 1 article reviews
c1000 touch thermal cycler with dual 48 48 fast reaction module - by Bioz Stars, 2026-09
98/100 stars
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cycler  (tiangen biotech co)
93
tiangen biotech co cycler
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Cycler, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/TGreat+Expert+Thermal+Cycler/pm41095256-149-37-38
Average 93 stars, based on 1 article reviews
cycler - by Bioz Stars, 2026-09
93/100 stars
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real time quantitative thermal cycler fmr3 system  (Vazyme Biotech Co)
95
Vazyme Biotech Co real time quantitative thermal cycler fmr3 system
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Real Time Quantitative Thermal Cycler Fmr3 System, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/Real-Time+Quantitative+Thermal+Cycler+FMR3/bio_rxiv__64898__2026__03__13__711744-338-19-18
Average 95 stars, based on 1 article reviews
real time quantitative thermal cycler fmr3 system - by Bioz Stars, 2026-09
95/100 stars
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dna engines peltier thermal cycler  (Bio-Rad)
99
Bio-Rad dna engines peltier thermal cycler
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Dna Engines Peltier Thermal Cycler, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/DNA+Engine+Tetrad+1+Thermal+Cycler+Firmware+Update/pm24314651-130-24-29
Average 99 stars, based on 1 article reviews
dna engines peltier thermal cycler - by Bioz Stars, 2026-09
99/100 stars
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techne prime thermal cycler  (Cole-Parmer)
93
Cole-Parmer techne prime thermal cycler
FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and <t>CD8</t> (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.
Techne Prime Thermal Cycler, supplied by Cole-Parmer, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cycler/PCRmax+Alpha+Cycler+2+Thermal+Cycler/pmc10673524-95-7-11
Average 93 stars, based on 1 article reviews
techne prime thermal cycler - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier

Image Search Results


FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and CD8 (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.

Journal: Cell Reports Medicine

Article Title: Follicular lymphoma B cells exhibit heterogeneous transcriptional states with associated somatic alterations and tumor microenvironments

doi: 10.1016/j.xcrm.2024.101443

Figure Lengend Snippet: FL B cell states shape their local immune profile (A) Uniform manifold approximation and projection of immune metaclusters from individually profiled FL patient CyTOF samples (n = 60). Clusters identified using Phenograph and are colored by manual annotation. (B) Heatmap of mean surface protein expression in each identified cluster mentioned in (A). Cluster identities listed next to their coordinating colors from (A). (C) Boxplot of immune content as percentage of live cells in each sample by their B cell group. (D) Correlation plot between the difference in CMI vs. INFM state coefficients (positive = higher CMI, negative = higher INFM) and the non-B immune cell percentage (% of live cells). A standard linear regression line is displayed with 95% CIs, along with ( r ) Pearson correlation, and representative p value. (E) (Left) Heatmap of correlation matrix between cell cluster abundances in all of the samples, depicting cellular communities (black boxes). Cell values from Pearson correlation. Dendrogram from correlation distance. (Right) Heatmap of Pearson correlation between cell abundance and sample B cell group state. Pearson p values are printed where p < 0.1. (F) Heatmap of Spearman correlation between single-sample gene set scoring, using SingScore of published GC stromal gene sets, and B cell group states (n = 54). Pearson p values are printed in cells where p < 0.1. (G) CODEX multiplexed immunofluorescence analysis of CD21 (white), CD20 (blue), Ki-67 (yellow), CD4 (green), and CD8 (magenta) on 1 sample from each B cell group. (Left) Baseline cell type abundance in each sample (% of live) from CyTOF. (Right) First 3 panels, stitched images equal to 10× magnification with 500-μm scale. White box delineating the magnified view in panel 4 of follicle and interfollicular region, with 200-μm scale.

Article Snippet: CD8-BX026 (C8/144B)—Atto 550-RX026 , Akoya , Catalog: 4250012, RRID: AB_2915960.

Techniques: Expressing, Immunofluorescence

Journal: Cell Reports Medicine

Article Title: Follicular lymphoma B cells exhibit heterogeneous transcriptional states with associated somatic alterations and tumor microenvironments

doi: 10.1016/j.xcrm.2024.101443

Figure Lengend Snippet:

Article Snippet: CD8-BX026 (C8/144B)—Atto 550-RX026 , Akoya , Catalog: 4250012, RRID: AB_2915960.

Techniques: Cell Isolation, Software, Multiplex Assay