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Image Search Results
Journal: Biomaterials
Article Title: The effect of stromal cell-derived factor-1α/heparin coating of biodegradable vascular grafts on the recruitment of both endothelial and smooth muscle progenitor cells for accelerated regeneration.
doi: 10.1016/j.biomaterials.2012.07.042
Figure Lengend Snippet: Fig. 3. En face immunostaining for CD31, CD34, CD45 and CXCR7 of the explanted grafts at 1-week after implantation. (AeI) En face immunostaining for CD31 (green) and CD34 (red) of proximal, middle and distal portions of untreated grafts (AeC), heparin-treated grafts (DeF) and heparin-SDF-1a-treated grafts (GeI). (JeL) En face immunostaining for CD45 (green) and CXCR7 (red) of the untreated control grafts (J), heparin-treated grafts (K) and heparin-SDF-1a-treated grafts (L). Arrows indicate CD45þ cells. Nuclei were stained by DAPI (blue). Scale bar ¼ 40 mm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet: Immunohistochemical staining was used to analyze the tissue sections with the following primary antibodies: SM-MHC (sc-79079, goat, Santa Cruz Biotechnology Inc.), CNN1 (1806-1, rabbit, Epitomics, Inc.), SMA (1184-1, rabbit, Epitomics, Inc.), elastin (ab21610, rabbit, Abcam Inc.), Collagen type I (ab34710, rabbit, Abcam Inc.), CD31 (550300, mouse, BD Biosciences), CD34 (AF4117, rat, R&D), CD133 (ab19898, rabbit, Abcam Inc.), CD45 (05-1410, mouse, Millipore),
Techniques: Immunostaining, Control, Staining