Journal: Theranostics

Article Title: Kupffer cells promote T-cell hepatitis by producing CXCL10 and limiting liver sinusoidal endothelial cell permeability

doi: 10.7150/thno.44960

Figure Lengend Snippet: Dynamic changes of immune cell infiltration, and cytokine & chemokine profile in the liver after the KC depletion in steady condition. (A) Numbers of infiltrating immune cells (monocytes, T cells, B cells, NKT cells and NK cells) in livers before ILY injection (0 h) or at 14 and 24 h after 3 ILY injections (100 ng/g, i.p. at 2 h intervals) to ihCD59 + and LysM-Cre + ihCD59 + mice (n=4 at 0 h and n=6 for all other time points). (B-D) RT2 Profiler PCR Array analysis of the hepatic cytokine and chemokine transcripts in the LysM-Cre + ihCD59 + and ihCD59 + mice at 2 h after 1 ILY injection (B, 100 ng/g, i.p., n = 2), and at 14 h (C) and 24 h (D) after 3 ILY injections (100 ng/g, i.p. at 2 h intervals, n = 2 for each time point). Scatter plot represents the relative transcript levels for each gene obtained from LysM-Cre + ihCD59 + mice plotted against the same gene from ihCD59 + mice. Genes with fold changes >2.0 are shown in the table on the right. (E) ELISA analysis of CXCL13 in serum collected from ihCD59 + and LysM-Cre + ihCD59 + mice (n = 3) at 8 h after 3 ILY injections (100 ng/g, i.p. at 2 h intervals). Data are shown as mean ± SEM. * P<0.05; ** P<0.01, as determined by unpaired two-tailed Student's t-test.

Article Snippet: In some experiments, serum CXCL13 level was measured using a mouse CXCL13/BLC/BCA-1 DuoSet Elisa Kit (R&D DY470, Minneapolis, MN).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation

doi: 10.3389/fimmu.2020.01204

Figure Lengend Snippet: Cytokine levels in serum samples and kidney tissue. Serum levels of pro-inflammatory cytokines were measured in models of uni- and bilateral renal IRI. CXCL13 levels significantly increased within 24 h in 30 min bilateral IRI as compared to 15 min IRI or baseline (BL) levels prior to IRI (A) . In unilateral IRI for 35 and 45 min a time-dependent increase of serum CXCL13 levels were observed 24 h after IRI (B) . To longitudinally determine the kinetics of CXCL13 release in 45 min unilateral IRI blood samples were taken at the indicated times. A maximum was measured 24 h after IRI (C) . MCP-1 (D) and IL-6 (E) were measured in comparison to sham surgery prior to IRI and 24 h after bilateral IRI. Both markers were significantly increased in comparison to baseline. 24 h after unilateral IRI a significant increase of CXCL13 (F) and of IL-6 (G) mRNA expression in renal tissue was observed in the 45 min unilateral IRI model ( n = 6–10 mice per group, one-way ANOVA, * p < 0.05; ** p < 0.01, *** p < 0.001). BL, baseline.

Article Snippet: CXCL13 serum levels were analyzed by ELISA (R&D Systems, MCX130) as described previously ( ).

Techniques: Comparison, Expressing

Journal: Frontiers in Immunology

Article Title: Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation

doi: 10.3389/fimmu.2020.01204

Figure Lengend Snippet: Serum CXCL13 levels in mouse ktx. Post ktx levels of serum CXCL13 at day 1 were significantly increased compared to baseline. A higher increase was observed in longer cold ischemia time (30 vs. 60 min cold ischemia time). Isogenic ktx with prolonged cold ischemia time of 60 min had significantly lower CXCL13 levels compared to allogenic ktx (A) . PAS stain at day 7 revealed enhanced cell infiltration in allogenic compared to isogenic ktx (B) . Double staining for CD3+ T-lymphocytes (green) and CD45R+ B-cells (red) was performed at day 7. More interstitial CD3+ T-lymphocytes were observed in allografts compared to isografts. Allografts exhibited scattered B-cells in interstitial tissue, but also clusters of CD45R+ cells. Isografts showed only few B cells in the interstitium at day 7 (B) (bar: 100 μm, n = 6 per group, one-way ANOVA * p < 0.05; ** p < 0.01; *** p < 0.001). BL, baseline.

Article Snippet: CXCL13 serum levels were analyzed by ELISA (R&D Systems, MCX130) as described previously ( ).

Techniques: Staining, Double Staining

Journal: Theranostics

Article Title: Kupffer cells promote T-cell hepatitis by producing CXCL10 and limiting liver sinusoidal endothelial cell permeability

doi: 10.7150/thno.44960

Figure Lengend Snippet: Dynamic changes of immune cell infiltration, and cytokine & chemokine profile in the liver after the KC depletion in steady condition. (A) Numbers of infiltrating immune cells (monocytes, T cells, B cells, NKT cells and NK cells) in livers before ILY injection (0 h) or at 14 and 24 h after 3 ILY injections (100 ng/g, i.p. at 2 h intervals) to ihCD59 + and LysM-Cre + ihCD59 + mice (n=4 at 0 h and n=6 for all other time points). (B-D) RT2 Profiler PCR Array analysis of the hepatic cytokine and chemokine transcripts in the LysM-Cre + ihCD59 + and ihCD59 + mice at 2 h after 1 ILY injection (B, 100 ng/g, i.p., n = 2), and at 14 h (C) and 24 h (D) after 3 ILY injections (100 ng/g, i.p. at 2 h intervals, n = 2 for each time point). Scatter plot represents the relative transcript levels for each gene obtained from LysM-Cre + ihCD59 + mice plotted against the same gene from ihCD59 + mice. Genes with fold changes >2.0 are shown in the table on the right. (E) ELISA analysis of CXCL13 in serum collected from ihCD59 + and LysM-Cre + ihCD59 + mice (n = 3) at 8 h after 3 ILY injections (100 ng/g, i.p. at 2 h intervals). Data are shown as mean ± SEM. * P<0.05; ** P<0.01, as determined by unpaired two-tailed Student's t-test.

Article Snippet: In some experiments, serum CXCL13 level was measured using a mouse CXCL13/BLC/BCA-1 DuoSet Elisa Kit (R&D DY470, Minneapolis, MN).

Techniques: Injection, Enzyme-linked Immunosorbent Assay, Two Tailed Test