Journal: Biochemical pharmacology

Article Title: Site-directed mutagenesis of the human adenosine A 2A receptor. Critical involvement of Glu 13 in agonist recognition

doi:

Figure Lengend Snippet: Approximated IC 50 values ( μ M) of agonists and antagonists obtained in radioligand binding studies with [ 3 H]XAC on the wild-type (WT) and E13Q mutant of the cloned human adenosine A 2A receptor ( n = 2, with individual IC 50 values differing less than 30%: {"type":"entrez-protein","attrs":{"text":"CGS21680","term_id":"878113053"}} CGS21680 alone: n = 3, IC 50 ± S.E.M.)

Article Snippet: [ 3 H] {"type":"entrez-protein","attrs":{"text":"CGS21680","term_id":"878113053"}} CGS21680 (41 Ci/mmol) and [ 3 H]XAC (109 Ci/mmol; custom synthesis) were from DuPont NEN (Dordrecht, Netherlands).

Techniques: Binding Assay, Mutagenesis, Clone Assay

Journal: eLife

Article Title: Enzyme activity and selectivity filter stability of ancient TRPM2 channels were simultaneously lost in early vertebrates

doi: 10.7554/eLife.44556

Figure Lengend Snippet:

Article Snippet: Gene ( Salpingoeca rosetta TRPM2) , srTRPM2, srNUDT9-H , General Biosystems, Inc. , NCBI: XP_004993318 , species-optimized synthetic genes.

Techniques: Plasmid Preparation, Mutagenesis, Avidin-Biotin Assay, Software