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Image Search Results
Journal: Experimental and Therapeutic Medicine
Article Title: Icariin‑curcumol promotes ferroptosis in prostate cancer cells through Nrf2/HO‑1 signaling
doi: 10.3892/etm.2024.12519
Figure Lengend Snippet: Screening of prostate cells and icariin-curcumol concentrations. The proliferation of (A) RWPE-2, (B) PC-3, (C) VCAP and (D) DU145 cell proliferation was detected using CCK8. (E) The Nrf2 and HO-1 levels in cells were analyzed by using western blotting. # P<0.05 vs. control; n=3. Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1.
Article Snippet: Icariin (cat. no. N1705; APeXBIO Technology LLC) and
Techniques: Western Blot, Control
Journal: Experimental and Therapeutic Medicine
Article Title: Icariin‑curcumol promotes ferroptosis in prostate cancer cells through Nrf2/HO‑1 signaling
doi: 10.3892/etm.2024.12519
Figure Lengend Snippet: Icariin-curcumol promotes ferroptosis in DU145 cells. (A) The p53, SLC7A11 and GPX4 (ferroptosis-related proteins) levels were analyzed using a western blotting. (B) p53, SLC7A11 and GPX4 levels were detected using RT-qPCR. (C) Intracellular Fe 2+ levels were assessed with an iron colorimetric assay kit. (D) The fluorescence intensity of ROS. (E) Flow cytometry of intracellular ROS production. (F) A GSH detection kit was utilized to detect intracellular GSH content. (G) The MDA level was assessed using an MDA detection kit. (H) The Nrf2 and HO-1 levels in cells were analyzed using RT-qPCR and western blotting. # P<0.05 vs. control; n=3. SLC7A11, solute carrier family 7 member 11; GPX4, glutathione peroxidase 4; RT-qPCR, reverse transcription-quantitative PCR; ROS, reactive oxygen species; GSH, glutathione; MDA, malondialdehyde; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1.
Article Snippet: Icariin (cat. no. N1705; APeXBIO Technology LLC) and
Techniques: Western Blot, Quantitative RT-PCR, Colorimetric Assay, Fluorescence, Flow Cytometry, Control, Reverse Transcription, Real-time Polymerase Chain Reaction
Journal: Experimental and Therapeutic Medicine
Article Title: Icariin‑curcumol promotes ferroptosis in prostate cancer cells through Nrf2/HO‑1 signaling
doi: 10.3892/etm.2024.12519
Figure Lengend Snippet: Icariin-curcumol promotes ferroptosis in DU145 cells through Nrf2/HO-1 signaling axis. (A) The Nrf2 and HO-1 levels in DU145 cells were analyzed using western blotting. (B) The Nrf2 and HO-1 levels in DU145 cells were assessed using RT-qPCR. (C) The proliferation ability of DU145 cells was examined using CCK8. (D) The p53, SLC7A11 and GPX4 levels in DU145 cells were tested using RT-qPCR. (E) Western blotting was used to detect the p53, SLC7A11 and GPX4 levels in DU145 cells. (F) Intracellular Fe 2+ levels were assessed with an iron colorimetric assay kit. (G) The fluorescence intensity of ROS. (H) Flow cytometry of intracellular ROS production. (I) A GSH detection kit was used to detect intracellular GSH content. (J) The MDA level in DU145 cells was tested using an MDA detection kit. n=3. # P<0.05 vs. control; & P<0.05 vs. icariin + curcumol + oe-NC. Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; SLC7A11, solute carrier family 7 member 11; GPX4, glutathione peroxidase 4; si small interfering; NC, negative control; oe, overexpression; GSH, glutathione; MDA, malondialdehyde.
Article Snippet: Icariin (cat. no. N1705; APeXBIO Technology LLC) and
Techniques: Western Blot, Quantitative RT-PCR, Colorimetric Assay, Fluorescence, Flow Cytometry, Control, Negative Control, Over Expression