Journal: Frontiers in Immunology

Article Title: Synergistic effects of platelet-rich fibrin and CTLA4Ig gene-transfected porcine skin on accelerating wound healing in a rat model of deep second-degree burns: a mechanistic study

doi: 10.3389/fimmu.2025.1756818

Figure Lengend Snippet: Temporal expression profiles of antioxidant enzymes CAT and SOD1. Immunofluorescence staining of (A) Catalase (CAT, red) and (B) Superoxide Dismutase 1 (SOD1, red) in wound tissues from the Vaseline group, PRF group, Pigskin group, and PRF+pig skin group at days 4, 7, 14, and 21 post-treatment. Cell nuclei are counterstained with DAPI (blue). The PRF+pig skin group shows the most pronounced and sustained enhancement in the expression of both antioxidant enzymes, particularly during the proliferative and remodeling phases (D7–D21), indicating a reinforced antioxidant defense system. Scale bar = 100 μm.

Article Snippet: After deparaffinization and antigen retrieval (similar to IHC), sections were permeabilized with 0.1% Triton X-100, blocked with 5% BSA, and incubated overnight at 4 °C with primary antibodies against CAT (1:200, Proteintech) and SOD1 (1:200, Proteintech).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Impact of adropin on superoxide dismutase expression in pancreatic β‐cells of normoglycemic and diabetic rats. (A) Immunofluorescence labeling with anti‐superoxide dismutase antibody and anti‐insulin antibodies showed expression of superoxide dismutase in pancreatic β‐cells. (B) Quantification of the histological analysis showed a significant (*** p < 0.001) decrease in superoxide dismutase distribution in pancreatic endocrine cells and a significant (**** p < 0.0001) decrease in its localization in β‐cells of rats with diabetes when compared to the normal group. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Labeling

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on superoxide dismutase expression in pancreatic α‐cells of normal and diabetic rats. (A) Immunofluorescence staining using anti‐superoxide dismutase and anti‐glucagon antibodies showed expression of superoxide dismutase in pancreatic α‐cells. (B) Quantification of the histological analysis showed a significant (** p < 0.01) decrease in superoxide dismutase distribution in the pancreatic endocrine cells of diabetic rats compared to the normal group. α‐Cell expression of superoxide dismutase did not change with adropin treatment among all groups. n = 6. Scale bar: 50 μm. Data analysis was done using the ANOVA test.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Expressing, Immunofluorescence, Staining

Journal: Animal Models and Experimental Medicine

Article Title: Adropin modulates pancreatic cell proliferation and glutathione levels in an animal model of type 1 diabetes mellitus

doi: 10.1002/ame2.70092

Figure Lengend Snippet: Effect of adropin on catalase, superoxide dismutase and total glutathione activities in the serum samples of healthy and diabetic rats. (A) Catalase activity was significantly decreased in diabetic rats compared to normal controls. Adropin slightly increased catalase in DMT group. (B) Superoxide dismutase was slightly increased in DMT compared to the diabetic treated with adropin. (C) Total glutathione was significantly raised with adropin treatment in DMT compared to DMUT. n = 4–6. Data analysis was done using the ANOVA test. * p < 0.05.

Article Snippet: Superoxide dismutase (rabbit) (1:500) , Rockland Immunochemicals, USA.

Techniques: Activity Assay

Journal: iScience

Article Title: Immuno-functionomics reveals geographical variation and a role for TLR8 in mRNA vaccine responses

doi: 10.1016/j.isci.2025.113839

Figure Lengend Snippet: Innate baseline functional response to PRR stimulation predicts immunogenicity of SARS-CoV-2 mRNA vaccination The baseline functional capacity of PBMCs of 18 SARS-CoV-naive European individuals was measured with priority on reactivity against endosomal TLR agonists (TLRs 3, 7, 8, and 9) and cytosolic sensors cGAS, STING, and RIG-1. The functional response was then compared to the frequencies of SARS-CoV-2-specific T and B cells and titers of SARS-CoV-2-specific antibodies. (A) Overview of the study where 2 doses of Moderna mRNA-1273 vaccination were administered and peripheral blood was obtained before vaccination and 2 weeks after the second dose. (B and C) Correlation heatmap reporting Spearman correlation coefficients (r) and p values for each comparison. Cutoff for significant correlations are r > 0.5 and p < 0.01. Baseline frequencies (B) or functional responses (C) of immune cell populations are compared to frequencies of SARS-CoV-2-specific T or B cells or titers of SARS-CoV-2-specific antibodies. (D) Representative Spearman correlation plots are shown for immune cell populations where the function correlated with the vaccine immunogenicity. (E) PBMCs were stimulated with TLR8 agonist ssRNA40, Moderna mRNA1273, and IL12/IL18, with and without TLR8 inhibitor CPT9a. Correlations were assessed with multiple Spearman correlations without correction for multiple testing. Significant correlations were depicted as ∗ p < 0.01 with r < 0.5. Statistical differences were further assessed with Wilcoxon signed-rank tests (E). ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001. Bar plots depict median with 95% confidence interval.

Article Snippet: For stimulations where the TLR8 inhibitor CU-CPT9a (Invivogen, USA) was added, cells were prior to stimulation pre-incubated with 10 μM of CU-CPT9a for 3 hours at 37°C.

Techniques: Functional Assay, Immunopeptidomics, Comparison

Journal: iScience

Article Title: Immuno-functionomics reveals geographical variation and a role for TLR8 in mRNA vaccine responses

doi: 10.1016/j.isci.2025.113839

Figure Lengend Snippet: Response to SARS-CoV-2 Comirnaty mRNA-XBB vaccine in vitro of rural Indonesians and Europeans The response to mRNA vaccines of the innate immune repertoire from rural Indonesians ( n = 8) and Europeans ( n = 12) was investigated by in vitro stimulation with Comirnaty mRNA-XBB and was compared to the response to TLR8. (A) Schematic representation of the experimental setup created in BioRender. Jochems, S. (2025) https://BioRender.com/tmya4yp . (B) Boxplots of immune populations that were functionally different between groups in response to Comirnaty mRNA-XBB and TLR8. (C) Spearman correlation plots are shown for immune cell populations where the response to TLR8 correlated with the Comirnaty mRNA-XBB vaccine immunogenicity. Statistical differences were assessed with multiple Mann-Whitney U tests, false discovery rate corrected. ∗ p < 0.05. Bar plots depict median with 95% confidence interval. Spearman correlation coefficients (r) and p values for each comparison are depicted in the correlation plots.

Article Snippet: For stimulations where the TLR8 inhibitor CU-CPT9a (Invivogen, USA) was added, cells were prior to stimulation pre-incubated with 10 μM of CU-CPT9a for 3 hours at 37°C.

Techniques: In Vitro, Vaccines, Immunopeptidomics, MANN-WHITNEY, Comparison