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A. muciniphila regulates the expression of immune factors. (A) The levels <t>of</t> <t>MMP-1,</t> MMP-9, <t>CTSB,</t> and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.
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Elabscience Biotechnology mouse ctsb assay kits
A. muciniphila regulates the expression of immune factors. (A) The levels <t>of</t> <t>MMP-1,</t> MMP-9, <t>CTSB,</t> and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.
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OriGene human ctsb
A. muciniphila regulates the expression of immune factors. (A) The levels <t>of</t> <t>MMP-1,</t> MMP-9, <t>CTSB,</t> and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.
Human Ctsb, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Atlas Antibodies ctsb
A. muciniphila regulates the expression of immune factors. (A) The levels <t>of</t> <t>MMP-1,</t> MMP-9, <t>CTSB,</t> and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.
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OriGene repair hdr
A. muciniphila regulates the expression of immune factors. (A) The levels <t>of</t> <t>MMP-1,</t> MMP-9, <t>CTSB,</t> and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.
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Image Search Results


A. muciniphila regulates the expression of immune factors. (A) The levels of MMP-1, MMP-9, CTSB, and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.

Journal: Frontiers in Microbiology

Article Title: Akkermansia muciniphila Alters Gut Microbiota and Immune System to Improve Cardiovascular Diseases in Murine Model

doi: 10.3389/fmicb.2022.906920

Figure Lengend Snippet: A. muciniphila regulates the expression of immune factors. (A) The levels of MMP-1, MMP-9, CTSB, and CTSL in abdominal aortic tissue were analyzed by ELISA. (B,C) The expression of IL-33 in abdominal aortic tissue was analyzed by IHC (scale bar = 25 μm; the magnification is 400×). (D) qRT-PCR was used to analyze IL-33 gene expression in abdominal aortic tissue. (E) The expression of IL-33 protein in abdominal aortic tissue was analyzed by Western blot. (F) The levels of IL-4, IL-6, IL-10, IL-17A, IFN-γ, and C-reactive protein (CRP) in peripheral blood were determined by ELISA. (G) The expression of PCNA, OPN, and α-SMA protein in abdominal aortic aneurysm tissue were detected by Western blot. Data were shown as means ± SD and analyzed by two-way ANOVA ( n = 10 mice/group). *Compared with the control group, P < 0.05; # compared with the AAA group, P < 0.05.

Article Snippet: According to the manufacturer’s instructions, the levels of MMP-1 (ml037721, Shanghai Meilian, Shanghai, China), MMP-9 (CSB-E08007m, CUSABIO, Wuhan, China), CTSB (CSB-EL006185MO, CUSABIO, Wuhan, China), CTSL (E-EL-M0251c, Elabscience, Wuhan, China), IL-4 (CSB-E04634m, CUSABIO, Wuhan, China), IL-6 (CSB-E04639m, CUSABIO, Wuhan, China), IL-10 (CSB-E04594m, CUSABIO, Wuhan, China), IL-17A (CSB-E04608m, CUSABIO, Wuhan, China), IFN-γ (CSB-E04578m, CUSABIO, Wuhan, China), and C-reactive protein (CRP, CSB-E07922r, CUSABIO, Wuhan, China) were analyzed by ELISA assay.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Gene Expression, Western Blot, Control