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Image Search Results
Journal: Circulation journal : official journal of the Japanese Circulation Society
Article Title: Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model.
doi: 10.1253/circj.cj-13-0534
Figure Lengend Snippet: Figure 3. Differentiation potentials of cardiac stem cells (CSC) in vitro. (A) CSCs cultured at a low (blue) or high (red) plating density were incubated with dexamethasone for 9 days. The expression levels of cardiomyocyte (cTnT), smooth muscle cell (PDGFRB), and endothelial cell (TIE2) markers were analyzed by qPCR during the culture period. (B–D) Immunostaining for dif- ferentiated cells on day 7. Scale bars=100 μm. (E–G) Quantitative analysis of the immunostaining. The results from 11–20 fields per group were examined. *P<0.05, **P<0.01 vs. “High” group. +++P<0.0001 vs. “High+GSI” group.
Article Snippet: Thus, reducing Notch signaling is important when culturing CSCs for clinical applications. (Circ J 2014; 78: 222 – 231) Key Words: Cardiac stem cells; Cell culture; Notch signaling ORIGINAL ARTICLE Regenerative Medicine Circulation Journal Vol.78, January 2014 Hs99999905_m1, P21: Hs00355782_m1, P53: Hs01034249_ m1, ETS1: Hs00901425_m1, TIE2: Hs00945155_m1, HES1: Hs00172878_m1, C-KIT: Hs00174029_m1, IL8: Hs99999034_ m1, VEGFA: Hs00900055_m1, cTnT:
Techniques: In Vitro, Cell Culture, Incubation, Expressing, Immunostaining
Journal: Stem cells international
Article Title: 5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells.
doi: 10.1155/2020/5162350
Figure Lengend Snippet: Figure 4: 5-AzaC treatment increased levels of cardiac troponin-T (cTnT) and α-sarcomeric actin (α-actin) in BM-VSELs. (a) Immunostaining for cTnT and α-actin in cardiomyocytes derived from 5-AzaC-induced differentiation of VSELs. The white arrow indicates positive cells. (b) Flow cytometric analysis demonstrated the percentages of cTnT- and α-actin-positive cells on different days.
Article Snippet: Cells were incubated overnight at 4°C with primary antibodies (1 : 200 dilution) against rat-specific cardiac proteins, including
Techniques: Immunostaining, Derivative Assay