Journal: Cell & bioscience

Article Title: Up-regulation of the Hippo pathway effector TAZ renders lung adenocarcinoma cells harboring EGFR-T790M mutation resistant to gefitinib.

doi: 10.1186/2045-3701-5-7

Figure Lengend Snippet: Figure 4 TAZ mediates EMT of gefitinib-resistant PC9/GR. (A) TAZ knockdown in PC9/GR cells resulted in a morphology change in PC9/GR by a light microscope. The spindle form of the PC9/GR-shNC cells changed to a round shape. The cell density of the clusters was obviously enhanced in the PC9/GR-shTAZ cells. (B) Cell lysates from PC9/GR and PC9 cells expressing vector, shTAZ and PEX2-TAZ were separated and probed with antibodies for epithelial marker and mesenchymal markers as indicated. TAZ knockdown in PC9/GR cells increased E-cadherin and decreased vimentin, CTGF and AXL. All data were normalized to GAPDH. (C) Luciferase reporter assay in 16HBE cells with TAZ or TAZ-S51A overexpression. 16HBE cells co-transfected with pGL3-CTGF/pGL3-AXL and pEX2-TAZ showed significantly elevated reporter activity compared with controls. Meanwhile, cells co-transfected with pEX2-TAZ-S51A, which disrupts the TEAD binding, exhibited decreased luciferase activity compared with pEX2-TAZ. Cells were harvested 48 h after transfection for measurement of luciferase activity in all experiments. (D) Luciferase reporter assay in PC9/GR cells. Total amounts of DNA and RNA were kept constant. Results are expressed as means ± SEM from three independent experiments. Statistical analyses were carried out using Student’s t-test.

Article Snippet: Nitrocellulose membranes were incubated with antibodies against TAZ, E-cadherin (1:200, Cell Signaling Technology, Inc.), Vimentin (1:200, abcam), CTGF (1:200, Proteintech Group, Inc.), AXL (1:200, abcam), tAKT, pAKT, tERK and pERK (1:100, Cell Signaling Technology, Inc.), GAPDH (1:5000, Santa Cruz Biotechnology) and histone H3 (1:2000, abcam) followed by horseradish peroxidase-linked secondary antibody.

Techniques: Knockdown, Light Microscopy, Expressing, Plasmid Preparation, Marker, Luciferase, Reporter Assay, Over Expression, Transfection, Activity Assay, Binding Assay

Journal: Journal of pharmacological sciences

Article Title: Oligo-peptide I-C-F-6 inhibits hepatic stellate cell activation and ameliorates CCl 4 -induced liver fibrosis by suppressing NF-κB signaling and Wnt/β-catenin signaling.

doi: 10.1016/j.jphs.2018.01.003

Figure Lengend Snippet: Fig. 3. The effects of oligo-peptide I-C-F-6 on HSC activation markers and cytokine levels. (A) The immunohistochemistry of TGF-b1 and a-SMA. Magnification, 200. (B) The effects of oligo-peptide I-C-F-6 on TGF-b1 and a-SMA levels in vitro. The data are expressed as the means ± SD of three independent experiments. *P < 0.05, **P < 0.01 compared with 0 mg/ mL. (C) The effects of oligo-peptide I-C-F-6 on TGF-b1 and a-SMA levels in vivo. (DeG) Serum levels of CTGF, TNF-a, VEGF, TGF-b1. Data are expressed as means ± SD (n ¼ 6e8). #P < 0.05, ##P < 0.01 compared with the control group; *P < 0.05, **P < 0.01 compared with the CCl4 group.

Article Snippet: The MMP-2, MMP-9, TIPM-1, CTGF, TGF-b1, TNF-a, and VEGF ELISA kits were purchased from Cusabio Biotech (Wuhan, China).

Techniques: Activation Assay, Immunohistochemistry, In Vitro, In Vivo, Control

Journal: Scientific Reports

Article Title: Human amniotic fluid stem cells can alleviate detrusor dysfunction caused by bladder outlet obstruction in rats

doi: 10.1038/s41598-022-10640-y

Figure Lengend Snippet: Schema of the experimental procedure. pBOO = Partial bladder outlet obstruction, PBS = Phosphate-buffered saline, hAFSCs = Human amniotic fluid stem cells, H&E = Hematoxylin and eosin, PCR = Polymerase chain reaction, HIF1α = Hypoxia inducible factor-1α, IL-1β = Interleukin-1β, TGF-β1 = Transforming growth factor-β1, CTGF = Connective tissue growth factor, α-SMA = α-smooth muscle actin, PGP9.5 = Protein gene product 9.5. Drawn by co-author, Yung-Hsin Huang.

Article Snippet: The assays codes of HIF1α (Rn01472831-m1), CCL2 (Rn00580555-m1), IL-1β (Rn00580432-m1), TGF-β1 (Rn00572010-m1), CTGF (Rn01537279-g1), α-SMA (Rn01759928-g1), collagen I (Rn01463848-m1), collagen III (Rn01437681-m1) and PGP9.5 (Rn00568258-m1) were obtained from manufacturer (Applied Biosystems, Oster City, CA, USA).

Techniques: Saline, Polymerase Chain Reaction

Journal: Scientific Reports

Article Title: Human amniotic fluid stem cells can alleviate detrusor dysfunction caused by bladder outlet obstruction in rats

doi: 10.1038/s41598-022-10640-y

Figure Lengend Snippet: Relative mRNA expressions of hypoxia inducible factor-1α (HIF1α, A ), CCL2 ( B ), interleukin-1β (IL-1β, C ), transforming growth factor-β1 (TGF-β1, D ), connective tissue growth factor (CTGF, E ), α-smooth muscle actin (α-SMA, F ), collagen I ( G ), collagen III ( H ) and protein gene product 9.5 (PGP9.5, I) in the bladder of control, pBOO + PBS and pBOO + hAFSCs treatment. After hAFSCs treatment, the mRNA expressions of CTGF and α-SMA are improved at 2 and 6 weeks after pBOO, but HIF1α, CCL2, IL-1β, TGF-β1, collagen I and collagen III are improved only at 2 weeks after pBOO. The mRNA levels of PGP9.5 are improved after hAFSCs treatment only at 6 weeks after pBOO. *: P < 0.05 vs. control, #: P < 0.05 vs. pBOO + PBS. N = 6 in each time point.

Article Snippet: The assays codes of HIF1α (Rn01472831-m1), CCL2 (Rn00580555-m1), IL-1β (Rn00580432-m1), TGF-β1 (Rn00572010-m1), CTGF (Rn01537279-g1), α-SMA (Rn01759928-g1), collagen I (Rn01463848-m1), collagen III (Rn01437681-m1) and PGP9.5 (Rn00568258-m1) were obtained from manufacturer (Applied Biosystems, Oster City, CA, USA).

Techniques: Control

Journal: Scientific Reports

Article Title: Human amniotic fluid stem cells can alleviate detrusor dysfunction caused by bladder outlet obstruction in rats

doi: 10.1038/s41598-022-10640-y

Figure Lengend Snippet: Expressions of the immunoreactivities of hypoxia inducible factor-1α (HIF1α, A ), CCL2 ( B ), interleukin-1β (IL-1β, C ), transforming growth factor-β1 (TGF-β1, D ), connective tissue growth factor (CTGF, E), α-smooth muscle actin (α-SMA, F), collagen I ( G ), collagen III ( H ) and protein gene product 9.5 (PGP9.5, I ) in the bladder of control, pBOO + PBS and pBOO + hAFSCs treatment. After hAFSCs treatment, the immunoreactivities of CTGF and α-SMA are improved at 2 and 6 weeks after pBOO, but HIF1α, CCL2, IL-1β, TGF-β1, collagen I and collagen III are improved only at 2 weeks after pBOO. The immunoreactivities of PGP9.5 are improved after hAFSCs treatment only at 6 weeks after pBOO. *: P < 0.05 vs. control, #: P < 0.05 vs. pBOO + PBS. N = 6 in each time point.

Article Snippet: The assays codes of HIF1α (Rn01472831-m1), CCL2 (Rn00580555-m1), IL-1β (Rn00580432-m1), TGF-β1 (Rn00572010-m1), CTGF (Rn01537279-g1), α-SMA (Rn01759928-g1), collagen I (Rn01463848-m1), collagen III (Rn01437681-m1) and PGP9.5 (Rn00568258-m1) were obtained from manufacturer (Applied Biosystems, Oster City, CA, USA).

Techniques: Control

Journal: Scientific Reports

Article Title: Human amniotic fluid stem cells can alleviate detrusor dysfunction caused by bladder outlet obstruction in rats

doi: 10.1038/s41598-022-10640-y

Figure Lengend Snippet: Temporal immunofluorescent expressions of CTGF and TGF-β1 in cultured detrusor cells of control, pBOO + PBS and pBOO + hAFSCs. Compared with the control, cultured detrusor cells of pBOO rat have higher CTGF immunoreactivity at 2 and 6 weeks after pBOO, and higher TGF-β1 immunoreactivity at 2 weeks after pBOO. All these changes can be improved after hAFSCs treatment. *: P < 0.05 vs. control, #: P < 0.05 vs. pBOO + PBS. N = 6 in each time point. Bar indicates 150 μm.

Article Snippet: The assays codes of HIF1α (Rn01472831-m1), CCL2 (Rn00580555-m1), IL-1β (Rn00580432-m1), TGF-β1 (Rn00572010-m1), CTGF (Rn01537279-g1), α-SMA (Rn01759928-g1), collagen I (Rn01463848-m1), collagen III (Rn01437681-m1) and PGP9.5 (Rn00568258-m1) were obtained from manufacturer (Applied Biosystems, Oster City, CA, USA).

Techniques: Cell Culture, Control