Journal: Journal of Inflammation Research

Article Title: Effects of Zofenopril and Thymoquinone in Cyclophosphamide-Induced Urotoxicity and Nephrotoxicity in Rats; The Value of Their Anti-Inflammatory and Antioxidant Properties

doi: 10.2147/JIR.S500375

Figure Lengend Snippet: Effect of ZOF and TQ alone and their combination on Kidney Injury Biomarkers. ( A ) KIM-1 and ( B ) Cys-C in Kidney Tissue Homogenate. One-way ANOVA multiple comparison was used followed by Tukey’s test. Number of animals per each group=8. *p=0.0175 PC vs MS, **p=0.007 PC vs ZOF, *p=0.04 PC vs TQ, **p=0.0055 PC vs ZOF+TQ in 5A; *p=0.019 PC vs NC, *p=0.031 PC vs ZOF+TQ, **p=0.007 PC vs MS and TQ, in 5B are statistically significant.

Article Snippet: The concentration of cystatin C (Cys-C) ng/mL and kidney injury molecule-1 (KIM-1) ng/mL in the supernatant of kidney tissue homogenate were determined using Rat CYS-C (Cat.No.E0145Ra) and KIM-1 (Cat.No.E0549Ra) BT LAB ELISA Kit from Bioassay technology laboratory, Shanghai, China strictly following the manufacturer’s instructions.

Techniques: Comparison

Journal: PLoS Pathogens

Article Title: Glycosaminoglycans and Sialylated Glycans Sequentially Facilitate Merkel Cell Polyomavirus Infectious Entry

doi: 10.1371/journal.ppat.1002161

Figure Lengend Snippet: Binding of Alexa Fluor 488-labeled capsids (top panel) or transducing activity of reporter vectors (bottom panel) on sialic acid-deficient Lec2 cells or Lec2 cells stably expressing the sialic acid transporter SLC35A1 (Lec2-mslc). The average values and standard error of the mean from three separate experiments are shown.

Article Snippet: The pMslc plasmid used to restore expression of SLC35A1 (accession number NM_006416) in Lec2 cells was created by transferring the human cDNA clone of SLC35A1 (OriGene, restriction enzymes XbaI and NcoI) into the expression cassette of pMONO-blasti-msc (InvivoGen, restriction enzymes AvrII and NcoI).

Techniques: Binding Assay, Labeling, Activity Assay, Stable Transfection, Expressing