cst 91882 Search Results


cd86  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc cd86
Bone loss and a significant increase in the number of M1 macrophages in the GA-ONFH group. ( A ) Gross view of femoral head samples from the control group and GA-ONFH group (longitudinal section); control group, n = 5; GA-ONFH group, n = 5. ( B , C ) Representative images of Masson staining ( n = 5 per group). Scale bar: 400 μm. Representative images of IHC staining for <t>CD86</t> in the femoral head and statistical analysis ( n = 5 per group). The red arrows indicate M1 macrophages. Scale bar: 100 μm. ( D ) Coronal (COR), transverse (TRA) and sagittal (SAG) sections of the femoral head were reconstructed from the micro-CT images ( n = 4 per group). Scale bar: 0.5 mm. ( E , F ) Representative images of IHC staining for COL1 and RUNX2 in the femoral head and statistical analysis ( n = 3 per group). Scale bar: 50 μm. ( G , H ) Representative images of IF staining showing CD86 (red)/DAPI (blue) in the mouse femoral head and statistical analysis ( n = 3 per group). Scale bar: 300 μm. ( I ) Representative images of multiple IF staining showing DAPI (blue)/CD86 (purple)/CD29 (red)/CD44 (green) in the mouse femoral head ( n = 3 independent experiments). Scale bar: 300 μm. The data are presented as the means ± SD. *** P < 0.001, ** P < 0.01. Two-tailed Student t-test
Cd86, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Bone loss and a significant increase in the number of M1 macrophages in the GA-ONFH group. ( A ) Gross view of femoral head samples from the control group and GA-ONFH group (longitudinal section); control group, n = 5; GA-ONFH group, n = 5. ( B , C ) Representative images of Masson staining ( n = 5 per group). Scale bar: 400 μm. Representative images of IHC staining for CD86 in the femoral head and statistical analysis ( n = 5 per group). The red arrows indicate M1 macrophages. Scale bar: 100 μm. ( D ) Coronal (COR), transverse (TRA) and sagittal (SAG) sections of the femoral head were reconstructed from the micro-CT images ( n = 4 per group). Scale bar: 0.5 mm. ( E , F ) Representative images of IHC staining for COL1 and RUNX2 in the femoral head and statistical analysis ( n = 3 per group). Scale bar: 50 μm. ( G , H ) Representative images of IF staining showing CD86 (red)/DAPI (blue) in the mouse femoral head and statistical analysis ( n = 3 per group). Scale bar: 300 μm. ( I ) Representative images of multiple IF staining showing DAPI (blue)/CD86 (purple)/CD29 (red)/CD44 (green) in the mouse femoral head ( n = 3 independent experiments). Scale bar: 300 μm. The data are presented as the means ± SD. *** P < 0.001, ** P < 0.01. Two-tailed Student t-test

Journal: Journal of Nanobiotechnology

Article Title: Exosomal miR-1a-3p derived from glucocorticoid-stimulated M1 macrophages promotes the adipogenic differentiation of BMSCs in glucocorticoid-associated osteonecrosis of the femoral head by targeting Cebpz

doi: 10.1186/s12951-024-02923-5

Figure Lengend Snippet: Bone loss and a significant increase in the number of M1 macrophages in the GA-ONFH group. ( A ) Gross view of femoral head samples from the control group and GA-ONFH group (longitudinal section); control group, n = 5; GA-ONFH group, n = 5. ( B , C ) Representative images of Masson staining ( n = 5 per group). Scale bar: 400 μm. Representative images of IHC staining for CD86 in the femoral head and statistical analysis ( n = 5 per group). The red arrows indicate M1 macrophages. Scale bar: 100 μm. ( D ) Coronal (COR), transverse (TRA) and sagittal (SAG) sections of the femoral head were reconstructed from the micro-CT images ( n = 4 per group). Scale bar: 0.5 mm. ( E , F ) Representative images of IHC staining for COL1 and RUNX2 in the femoral head and statistical analysis ( n = 3 per group). Scale bar: 50 μm. ( G , H ) Representative images of IF staining showing CD86 (red)/DAPI (blue) in the mouse femoral head and statistical analysis ( n = 3 per group). Scale bar: 300 μm. ( I ) Representative images of multiple IF staining showing DAPI (blue)/CD86 (purple)/CD29 (red)/CD44 (green) in the mouse femoral head ( n = 3 independent experiments). Scale bar: 300 μm. The data are presented as the means ± SD. *** P < 0.001, ** P < 0.01. Two-tailed Student t-test

Article Snippet: In this study, CD86 (91882, CST) was used to label M1 macrophages, while CD29 (ab30394, Abcam) and CD44 (5640, CST) were used together to label and localize BMSCs.

Techniques: Control, Staining, Immunohistochemistry, Micro-CT, Two Tailed Test

M1 macrophages inhibit the osteogenic differentiation of BMSCs. ( A ) The M1 macrophage surface markers CD86 and CD11b were analysed via flow cytometry ( n = 3 independent experiments). ( B ) Diagram of M1 macrophage and BMSC coculture in vitro . ( C ) The mRNA levels of osteogenesis-related genes in BMSCs were determined via RT‒qPCR analyses ( n = 3 independent experiments). ( D ) Assessment of COL1a1 ( n = 3 independent experiments), RUNX2 ( n = 4 independent experiments) and BMP2 ( n = 4 independent experiments) expression in BMSCs from different treatment groups by Western blotting, with β-actin used for normalization and quantitative analysis by ImageJ. ( E ) Mineralization in treated BMSCs was evaluated by alizarin red staining and ALP staining ( n = 3 independent experiments). Scale bar: 100 μm. ( F ) Statistical data of Alizarin red staining. ( G ) Statistical data of the ALP activity assay ( n = 3 independent experiments). The data are presented as the means ± SD. ** P < 0.01, * P < 0.05, ns = not significant. One-way ANOVA followed by Holm-Sidak multiple comparison test

Journal: Journal of Nanobiotechnology

Article Title: Exosomal miR-1a-3p derived from glucocorticoid-stimulated M1 macrophages promotes the adipogenic differentiation of BMSCs in glucocorticoid-associated osteonecrosis of the femoral head by targeting Cebpz

doi: 10.1186/s12951-024-02923-5

Figure Lengend Snippet: M1 macrophages inhibit the osteogenic differentiation of BMSCs. ( A ) The M1 macrophage surface markers CD86 and CD11b were analysed via flow cytometry ( n = 3 independent experiments). ( B ) Diagram of M1 macrophage and BMSC coculture in vitro . ( C ) The mRNA levels of osteogenesis-related genes in BMSCs were determined via RT‒qPCR analyses ( n = 3 independent experiments). ( D ) Assessment of COL1a1 ( n = 3 independent experiments), RUNX2 ( n = 4 independent experiments) and BMP2 ( n = 4 independent experiments) expression in BMSCs from different treatment groups by Western blotting, with β-actin used for normalization and quantitative analysis by ImageJ. ( E ) Mineralization in treated BMSCs was evaluated by alizarin red staining and ALP staining ( n = 3 independent experiments). Scale bar: 100 μm. ( F ) Statistical data of Alizarin red staining. ( G ) Statistical data of the ALP activity assay ( n = 3 independent experiments). The data are presented as the means ± SD. ** P < 0.01, * P < 0.05, ns = not significant. One-way ANOVA followed by Holm-Sidak multiple comparison test

Article Snippet: In this study, CD86 (91882, CST) was used to label M1 macrophages, while CD29 (ab30394, Abcam) and CD44 (5640, CST) were used together to label and localize BMSCs.

Techniques: Flow Cytometry, In Vitro, Expressing, Western Blot, Staining, ALP Activity Assay, Comparison