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Image Search Results
Journal: Scientific Reports
Article Title: DGCR8 regulates multiple processes of transcription coupled nucleotide excision repair
doi: 10.1038/s41598-026-38338-5
Figure Lengend Snippet: S153A mutation disrupts UV-induced interactions between DGCR8 and TC-NER factors in MEFs. (a, b) Quantification and representative PLA images showing CSB–CSA ( a ) and DGCR8–CSB ( b ) interactions in MEFs. Wild-type, heterozygous (wt/S153A), and homozygous (S153A/S153A) MEFs were irradiated with 10 J/m 2 UV-C (purple dots) or left untreated (gray dots), followed by 2 hours of recovery. PLA signals (red) were detected in nuclei counterstained with DAPI (blue). Horizontal black bars indicate the median of each group. Asterisks denote significant differences relative to non-irradiated or between genotypes (*** p < 0.001). White scale bars, 10 μm.
Article Snippet: The following commercial antibodies were also used: DGCR8 (10996-1-AP, Proteintech; sc-377249, Santa Cruz), RNA polymerase II (CDT-pS5, sc-55492[F-12], Santa Cruz), CSB (sc-25370, Santa Cruz),
Techniques: Mutagenesis, Irradiation