Journal: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry

Article Title: Selective adsorption of water-soluble ionic compounds by an interval immobilization technique based on molecular imprinting.

doi: 10.2116/analsci.24.1633

Figure Lengend Snippet: Fig. 3 Ion-exchange capacity and adsorption ratio of dcSTX after batch evaluation. The histograms denote the adsorption ratio and the dotted line represents the ion-exchange capacity.

Article Snippet: Decarbamoyl saxitoxin (dcSTX) was purchased from National Research Council Canada.

Techniques: Adsorption

Journal: PLoS ONE

Article Title: Over-Expressed miR-224 Promotes the Progression of Cervical Cancer via Targeting RASSF8

doi: 10.1371/journal.pone.0162378

Figure Lengend Snippet: (A) Wound healing assay was implemented in cervical cancer cell line SiHa and CaSki after transfected with 50 nM of miR-224 mimic or negative control (miR-NC). The wound healing was measured at the time points as indicated. Bars represented the average percentage of wound healing ±s.d. ** P<0.01. (B) Cell invasion capabilities were measured in SiHa and CaSki cells with transwell chambers, as described in materials and methods. Photos were representative fields of invasive cells on the membrane with a 200-fold magnification. Bar graphs represented the average number of cells per field on the underside of the membrane ±s.d. ** P<0.01.

Article Snippet: SiHa and CaSki cell lines, purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cell bank of Chinese Academy of Science in Shanghai respectively, were cultured at 37°C and 5% CO2 in a humidified incubator in Dulbecco’s modified Eagle’s medium (DMEM) and 1640 medium separately.

Techniques: Wound Healing Assay, Transfection, Negative Control, Membrane

Journal: PLoS ONE

Article Title: Over-Expressed miR-224 Promotes the Progression of Cervical Cancer via Targeting RASSF8

doi: 10.1371/journal.pone.0162378

Figure Lengend Snippet: (A,B) At 72h after transfected with 100 nM of miRNA mimic and negative control, the endogenous protein levels of RASSF8 in SiHa and CaSki cells were measured by western blot. Bars indicated the relative protein levels that were normalized to GAPDH. Data were presented as mean±s.d. (n¼3) *P<0.05, **P<0.01. (C) Putative miR-224-binding site in the RASSF83'-UTR mutation was generated by mutating 3nt that is recognized by miR-224. Either wild-type (WT) or mutant RASSF8 3'-UTR was subcloned into the dual-luciferase reporter vector. (D) The luciferase reporter vector containing wild (wild type) RASSF8 3’-UTR or mutant RASSF8 3’-UTR was cotransfected into SiHa cells with miR-224 mimic or miRNA negative control. (E) Firefly luciferase activities were determined at 48h postransfection and normalized to Renilla luciferase. Each column represented the mean±s.d. of three independent experiments. **P <0.01.

Article Snippet: SiHa and CaSki cell lines, purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cell bank of Chinese Academy of Science in Shanghai respectively, were cultured at 37°C and 5% CO2 in a humidified incubator in Dulbecco’s modified Eagle’s medium (DMEM) and 1640 medium separately.

Techniques: Transfection, Negative Control, Western Blot, Binding Assay, Mutagenesis, Generated, Luciferase, Plasmid Preparation

Journal: PLoS ONE

Article Title: Over-Expressed miR-224 Promotes the Progression of Cervical Cancer via Targeting RASSF8

doi: 10.1371/journal.pone.0162378

Figure Lengend Snippet: (A) Specific siRNA targeting RASSF8 suppressed RASSF8 protein expression. SiHa and CaSki cells were transfected with 50 nmol of siRNA RASSF8 or siRNA-negative control (siR-NC) and the transfection efficiency were assessed. RASSF8 protein expression levels were determined by western blot analysis. GAPDH was served as the internal control. (B)Representative images of wound healing were taken at 0h, 24h, and 48h after the wound scratched. Data are presented as mean±s.d. *P<0.05, **P<0.01. (C) The number of invaded cells following RASSF8 knockdown using specific siRNA targeting RASSF8(siR-RASSF8-a) versus negative control (siR-NC) were shown by stained with crystal violet. Bars indicated the invasion capability compared with that of negative control ± s.d*P<0.05, **P<0.01.

Article Snippet: SiHa and CaSki cell lines, purchased from the American Type Culture Collection (ATCC, Manassas, VA) and cell bank of Chinese Academy of Science in Shanghai respectively, were cultured at 37°C and 5% CO2 in a humidified incubator in Dulbecco’s modified Eagle’s medium (DMEM) and 1640 medium separately.

Techniques: Expressing, Transfection, Negative Control, Western Blot, Control, Knockdown, Staining