crl_1555 Search Results


a431 human epithelial carcinoma cell lines  (ATCC)
98
ATCC a431 human epithelial carcinoma cell lines
Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on <t>A431-GPC3</t> cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.
A431 Human Epithelial Carcinoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crl_1555/A-431/pm24492943-47-3-13
Average 98 stars, based on 1 article reviews
a431 human epithelial carcinoma cell lines - by Bioz Stars, 2026-10
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crl-1555  (LGC Standards)
98
LGC Standards crl-1555
Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on <t>A431-GPC3</t> cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.
Crl 1555, supplied by LGC Standards, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crl_1555/crl-1555/custom%40crl-1555%4038768097
Average 98 stars, based on 1 article reviews
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human epidermoid carcinoma cells  (ATCC)
96
ATCC human epidermoid carcinoma cells
Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on <t>A431-GPC3</t> cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.
Human Epidermoid Carcinoma Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crl_1555/A-431%3B+Epidermoid+Carcinoma%3B+Human/pm41614195-296-109-121
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human epidermoid carcinoma cells - by Bioz Stars, 2026-10
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a431 luc2  (ATCC)
93
ATCC a431 luc2
Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on <t>A431-GPC3</t> cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.
A431 Luc2, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crl_1555/A-431-Luc2/10__1158_slash_0008___5472__can___15___1232-88-0-8
Average 93 stars, based on 1 article reviews
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origin antibacterial activity  (ATCC)
94
ATCC origin antibacterial activity
Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on <t>A431-GPC3</t> cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.
Origin Antibacterial Activity, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crl_1555/Actinobacillus+suis+van+Dorssen+and+Jaartsveld/10__1128_slash_aem__68__11__5704___5710__2002-121-23-32
Average 94 stars, based on 1 article reviews
origin antibacterial activity - by Bioz Stars, 2026-10
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ms2 bacteriophage host  (ATCC)
94
ATCC ms2 bacteriophage host
Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on <t>A431-GPC3</t> cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.
Ms2 Bacteriophage Host, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/crl_1555/Actinobacillus+suis+van+Dorssen+and+Jaartsveld/us10029926-124-0-3
Average 94 stars, based on 1 article reviews
ms2 bacteriophage host - by Bioz Stars, 2026-10
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Image Search Results


Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on A431-GPC3 cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.

Journal: Hepatology (Baltimore, Md.)

Article Title: Inactivation of Wnt signaling by a human antibody that recognizes the heparan sulfate chains of glypican-3 for liver cancer therapy.

doi: 10.1002/hep.26996

Figure Lengend Snippet: Fig. 2. Isolation of a human antibody targeting HS chains of GPC3. (A) The binding affinity of HS20 on GPC3-his. (B) The binding affinity of HS20 on A431-GPC3 cells. (C) Flow cytometry analysis of HS20 on A431 and A431-GPC3 cells. (D) Binding properties of HS20 on GPC3, GPC3DHS, or irrelevant human Fc protein IAB. 1G12, a commercial mouse antibody against GPC3. (E) Immunoprecipitation of GPC3 in A431- GPC3 cells, Hep3B, and Hep3B GPC3 knockdown cells. Cell lysates incubated with HS20 or human IgG for immunoprecipitation. The immuno- precipitated complex was detected by 1G12. Black arrow indicates the GPC3 core protein and the bracket indicates glycosylated GPC3. Wt: wild-type; ko: knockdown. GPC3 knockdown efficiency is shown in Fig. 5C. (F) Competitive ELISA. Different concentrations of GPC3 or GPC3DHS were preincubated with HS20 (0.1 nM) for 1 hour. The mixture was added into the GPC3-coated plate to measure the binding of HS20/GPC3. CD22 was used as the negative control. The data represent the mean 6 SD.

Article Snippet: HepG2, Hep3B, and A431 (human epithelial carcinoma) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Isolation, Binding Assay, Flow Cytometry, Immunoprecipitation, Knockdown, Incubation, Competitive ELISA, Negative Control

Fig. 3. Both sulfated and nonsulfated regions of HS chain are involved in HS20 binding. (A) Treatment of GPC3-expressing cells with heparinase I, II, and III. A431-GPC3 cells were treated with different heparinases for 2 hours at 37C. Cells were then stained with HS20 (5 lg/mL) or YP7 (1 lg/mL) for flow cytometry. (B) Statistical analy- sis of three independent experiments. The binding fluorescence signals of untreated cells represent 100%. The data represent the mean 6 SD of three independent experiments (**P < 0.01 and #P > 0.05).

Journal: Hepatology (Baltimore, Md.)

Article Title: Inactivation of Wnt signaling by a human antibody that recognizes the heparan sulfate chains of glypican-3 for liver cancer therapy.

doi: 10.1002/hep.26996

Figure Lengend Snippet: Fig. 3. Both sulfated and nonsulfated regions of HS chain are involved in HS20 binding. (A) Treatment of GPC3-expressing cells with heparinase I, II, and III. A431-GPC3 cells were treated with different heparinases for 2 hours at 37C. Cells were then stained with HS20 (5 lg/mL) or YP7 (1 lg/mL) for flow cytometry. (B) Statistical analy- sis of three independent experiments. The binding fluorescence signals of untreated cells represent 100%. The data represent the mean 6 SD of three independent experiments (**P < 0.01 and #P > 0.05).

Article Snippet: HepG2, Hep3B, and A431 (human epithelial carcinoma) cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Binding Assay, Expressing, Staining, Cytometry