Journal: Journal of Medicinal Chemistry

Article Title: Design and Preclinical Evaluation of First 68 Ga-Labeled Cathepsin D‑Targeted Radiotracers

doi: 10.1021/acs.jmedchem.5c02151

Figure Lengend Snippet: Binding modes of the compounds NOTA-FZCD-1/2/3 with CTSD protein (PDB ID: 6QCB) (a) Cocrystal structures of NOTA-FZCD-1 (Left), NOTA-FZCD-2 (Center), and NOTA-FZCD-3 (Right) bound to the domain of human CTSD. (b) Cross-section structures of NOTA-FZCD-1 (Left), NOTA-FZCD-2 (Center), and NOTA-FZCD-3 (Right) bound to the domain of CTSD. (c) Binding modes of NOTA-FZCD-1 (left), NOTA-FZCD-2 (center), and NOTA-FZCD-3 (right) bound to the domain of CTSD. (d) H-bond interactions between NOTA-FZCD-1 (left), NOTA-FZCD-2 (center), and NOTA-FZCD-3 (right) bound to the domain of CTSD.

Article Snippet: The CTSD protein was purchased from MedChemExpress (China; HY-P7748A).

Techniques: Binding Assay

Journal: Journal of Medicinal Chemistry

Article Title: Design and Preclinical Evaluation of First 68 Ga-Labeled Cathepsin D‑Targeted Radiotracers

doi: 10.1021/acs.jmedchem.5c02151

Figure Lengend Snippet: CTSD expression and cellular uptake analysis of the optimized CTSD-targeting radiotracer [ 68 Ga]­Ga-NOTA-FZCD-3 (a) Western blotting of CTSD expression across breast cancer cell lines (MDA-MB-468, MCF-7, MDA-MB-415, and MDA-MB-175-VII, grayscale ratios of the expression of CTSD protein/reference protein were shown by a bubble plot). (b, c) Time-dependent cellular uptake kinetics of [ 68 Ga]­Ga-NOTA-FZCD-3 in MDA-MB-468, MCF-7, MDA-MB-415, and MDA-MB-175VII cell lines and MDA-MB-175VII with block. CTSD: cathepsin D.

Article Snippet: The CTSD protein was purchased from MedChemExpress (China; HY-P7748A).

Techniques: Expressing, Western Blot, Blocking Assay

Journal: bioRxiv

Article Title: RocS drives chromosome segregation and nucleoid occlusion in Streptococcus pneumoniae

doi: 10.1101/359943

Figure Lengend Snippet: Detection of CPS and DNA in (a) cpsD-3YE and cpsD-3YE-ΔrocS and (b) cpsD-3YF and cpsD-3YF-ΔrocS . Phase contrast (grey), CPS (red), DAPI (blue) and overlays are shown on the left. White arrows show CPS production defects, white arrowheads show anucleate cells and black arrowheads show nucleoid segregation defects. Scale bar, 1 µm. The corresponding percentage of anucleate cells are shown in the middle. (Two population proportions test: **** P < 0.0001). The corresponding distribution of the cell length are shown on the right. nT indicates the number of cells analyzed from 3 independent experiments and standard errors are indicated with error bars.

Article Snippet: Construction of the pGBDU- cpsD bait plasmid and expressing CpsD fused to the DNA-binding domain of Gal4 (BD) was described in ( ).

Techniques:

Journal: bioRxiv

Article Title: RocS drives chromosome segregation and nucleoid occlusion in Streptococcus pneumoniae

doi: 10.1101/359943

Figure Lengend Snippet: When CpsD is autophosphorylated, capsule is properly produced and RocS and ParB actively contribute to chromosome segregation (viable encapsulated progeny). By contrast, non-phosphorylated CpsD hinders both capsule synthesis and chromosome segregation inducing a division block. The deletion of rocS alleviates the division block and results in uncontrolled cell constriction with improper chromosome segregation (pinching and asymmetric distribution) leading to non-viable progeny. ParB, RocS, CpsD and its transmembrane activator CpsC are indicated by blue, yellow, brown and pink circles, respectively. Red "P" and the turquoise star indicate CpsD autophosphorylation and the oriC region, respectively. Capsule is shown in light (new capsule produced during cell division) and dark (inherit from the mother cell) red.

Article Snippet: Construction of the pGBDU- cpsD bait plasmid and expressing CpsD fused to the DNA-binding domain of Gal4 (BD) was described in ( ).

Techniques: Produced, Blocking Assay