Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1.

doi: 10.4049/jimmunol.1201599

Figure Lengend Snippet: FIGURE 3. cPLA2 inhibitors suppress air- way neutrophilia. (A) Varying concentrations of AACOCF3 were administered 30 min before the secondary airway challenge. (B) Then 0.4 nmol of cPLA2 siRNA or control siRNA was administered i.v. 24 h before the secondary airway challenge, and cPLA2 protein and phospho-cPLA2 were detected 30 min after the secondary airway challenge. (C) Mice were pretreated with siRNA, and the number of air- way neutrophils was measured 8 h after the secondary airway challenge. Data represent (A, C) the mean 6 SD and (B) a representative of three separate experiments (n = 3–5 per group). *p , 0.05 versus control group, **p , 0.05 versus OVA group.

Article Snippet: Small interfering RNA (siRNA) strands for cPLA2 and controls were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Control

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1.

doi: 10.4049/jimmunol.1201599

Figure Lengend Snippet: FIGURE 4. LTB4 induces airway neutrophilia and Gln inhibits LTB4 production. (A) Varying concentrations of the 5-LO inhibitors AA-861 and MK-886 were administered 1 h before the secondary airway challenge. (B) BAL fluid levels of LTB4 were determined after a second OVA airway challenge at the time indicated. (C) Effects of Gln and cPLA2 siRNA on BAL fluid levels of LTB4 at 1 h. (D) Varying concentrations of LTB4 were instilled i.t. 1 h after the secondary airway challenge. (E) The Gln-treated group was instilled i.t. with 1 mg of LTB4 at 1 h. The number of airway neutrophils was measured at 8 h. Data represent the mean 6 SD of three to five separate experiments (n = 5 per group).

Article Snippet: Small interfering RNA (siRNA) strands for cPLA2 and controls were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques:

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1.

doi: 10.4049/jimmunol.1201599

Figure Lengend Snippet: FIGURE 5. Gln inhibits the phosphorylation of cPLA2 and p38, and p38 inhibitor inhibits cPLA2 phosphorylation and airway neutrophilia. (A and B) Gln and Ala were administered 20 min post challenge. SB202190 (5 mg/kg) was injected i.p., and (C) the levels of cPLA2 phosphorylation and (D) neutrophil numbers in BAL fluid were measured 30 min and 8 h after the secondary airway challenge, respectively. (E) SB202190 was injected i.p. and BAL levels of MIP-2, KC, CXCL5, and LTB4 were measured at 2 h. (A–C) A representative of three independent experiments with three to five mice per time point per experiment is shown. (D and E) Data represent the mean 6 SD of two separate experiments (n = 5 per group). *p , 0.05 versus OVA group.

Article Snippet: Small interfering RNA (siRNA) strands for cPLA2 and controls were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Phospho-proteomics, Injection

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Glutamine suppresses airway neutrophilia by blocking cytosolic phospholipase A(2) via an induction of MAPK phosphatase-1.

doi: 10.4049/jimmunol.1201599

Figure Lengend Snippet: FIGURE 6. Gln-induced inhibition of p38 is asso- ciated with the early induction of MKP-1. (A) Gln and Ala were administered 20 min post challenge. (B) MKP-1 siRNA and control siRNA were administered i.v. 24 h before the secondary airway challenge. MKP-1 was measured at 90 min. (C) Then 0.4 nmol of siRNAs was given, and phospho-p38, phospho-cPLA2, and MKP-1 induction were measured at 30 min. A repre- sentative of two or three independent experiments with three to five mice per time point per experiment is shown. **p , 0.05 versus OVA group.

Article Snippet: Small interfering RNA (siRNA) strands for cPLA2 and controls were obtained from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Inhibition, Control

Journal: Cell Regeneration

Article Title: MRG15 decline in aged/injured MuSCs hinders regeneration via differentiation defects

doi: 10.1186/s13619-026-00279-9

Figure Lengend Snippet: MRG15 interacts with MyoD to regulate histone acetylation and transcription of myogenic genes. A Metascape databases were used to predict the transcription factors interacting with MRG15. B Venn diagrams showing the overlapping (216 genes) between the H4K16 acetylation (ChIP-Seq) target (9930) and the down-regulated genes (1460) among RNA-seq. C GO analysis of the above 216 genes revealed an extreme enrichment of skeletal muscle tissue development terms. D Enrichment of canonical MyoD motif in the above binding regions. E and F Co-IP assays showing the interactions between MRG15 and MyoD ( E ), or TIP60 ( F ) in C2C12. G ChIP-PCR analysis of H4K16ac occupancy at the promoter regions of myogenic genes in sg Mrg15 versus sgCtrl-C2C12 cells. Data are presented as percentage of input (% Input). H qPCR analysis of myogenic genes expression in sg Mrg15 or sgCtrl-C2C12 ( n = 3). Means ± SEM are shown. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: The whole-cell lysates obtained by centrifugation (with equal concentration of protein in different samples) were incubated with 1 μg of MyoD1 (M3512, Dako) or MRG15 (55257–1-AP, Proteintech), or TIP60 (10827–1-AP, Proteintech), antibody for 1.5 h at 4 °C with rotation followed by binding to Protein A/G PLUS-Agarose (sc-2003, Santa Cruz) for overnight at 4 °C.

Techniques: ChIP-sequencing, RNA Sequencing, Binding Assay, Co-Immunoprecipitation Assay, Expressing