Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: Ferroptosis and vascular arrest are present in the rat model of BPD induced by hyperoxia. A Comparison of body size and lung volume of rats after euthanasia; B Weight plot of rats in CON and BPD groups; C HE staining of rat lung tissue, scale bar: 50 μm; D Determination of mRNA contents of PTGS2, SLC7A11 and GPX4 in rat lung tissues, GAPDH was used as an internal control; E Quantitative analysis of radial alveolar count in rat lung tissues by HE staining; F - G . Protein expression and gray value quantitative analysis of PTGS2, SLC7A11 and GPX4 in lung tissue of rats, GAPDH was used as a whole protein internal control; The levels of LPO, MDA, SOD and GSH in serum of ( H - K ). L Determination of VEGFA and CD31 mRNA content in rat lung tissue, GAPDH was used as internal control; Protein expression and gray value quantification of VEGFA and CD31 in lung tissues of ( M - N ). GAPDH was used as a whole protein internal control. N = 6, p < 0.05 for statistically significant (* p < 0.05, * * p < 0.01, * * * p < 0.001, * * * * p < 0.0001)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Comparison, Staining, Control, Expressing

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: SP inhibited ferroptosis in BPD rats. A - C GPX4, SLC7A11 and PTGS2 mRNA levels in lung tissue of rats; D - E The protein contents of GPX4, SLC7A11 and PTGS2 in lung tissue of rats were detected, GAPDH was used as the whole protein internal control, and the gray value was quantified. F - K The contents of LPO, MDA, 4-HNE, SOD, GSH and GPX4 in serum of rats were detected. N = 6, p < 0.05 for statistically significant (* * p < 0.01, * * * p < 0.001, * * * * p < 0.0001)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Control

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: SP alleviates ferroptosis of HUVECs. A - C RT-PCR assay of HUVEC cells with GAPDH as internal control; D - E PTGS2, SLC7A11 and GPX4 proteins in HUVEC cells were detected, GAPDH was used as the whole protein internal reference and quantified by gray value. F -HUVEC LPO fluorescence staining, scale scale =100 μm, This fluorescent probe can emit red fluorescence under normal circumstances, and the fluorescence will change from red to green as lipid peroxidation occurs. The formation of lipid peroxides can be detected with high sensitivity through the fluorescence intensities of red and green; G HUVEC ROS fluorescence staining and quantification, scale scale =100 μm; H , I Quantification of LPO and ROS; J GSH measurement of HUVEC. N =6, p <0.05 was considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001, ns: no difference)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Fluorescence, Staining

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: Effect of SLC7A11 knockdown on SP intervention. A - C SLC7A11 siRNA knockdown efficiency was detected by RT-qPCR and Western blot; D - E PTGS2, CD31 and VEGFA protein detection and gray value quantification of HUVECs, GAPDH was used as an internal control; F - H , J Fluorescence plots and quantification of LPO and ROS of HUVECs, scale scale =100 μm, This fluorescent probe can emit red fluorescence under normal circumstances, and the fluorescence will change from red to green as lipid peroxidation occurs. The formation of lipid peroxides can be detected with high sensitivity through the fluorescence intensities of red and green.; I GSH measurement of HUVEC. Two-sided unpaired t test was used for comparison between the two groups. N =6, p <0.05 was considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001, ns: no difference)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Knockdown, Quantitative RT-PCR, Western Blot, Control, Fluorescence, Comparison

Journal: Respiratory Research

Article Title: Sodium propionate alleviates bronchopulmonary dysplasia by inhibiting ferroptosis through the SLC7A11/GPX4 pathway in pulmonary endothelial cells

doi: 10.1186/s12931-026-03535-3

Figure Lengend Snippet: Effect of GPX4 knockdown on SP intervention. A - C GPX4 siRNA knockdown efficiency was detected by RT-PCR and western blot; D - E PTGS2, CD31 and VEGFA protein detection and gray value quantification of HUVECs, GAPDH was used as an internal control; F - H , J Fluorescence plots and quantification of LPO and ROS of HUVEC, scale scale =100 μm, This fluorescent probe can emit red fluorescence under normal circumstances, and the fluorescence will change from red to green as lipid peroxidation occurs. The formation of lipid peroxides can be detected with high sensitivity through the fluorescence intensities of red and green.; I GSH measurement of HUVECs. Two-sided unpaired t test was used for comparison between the two groups. N =6, p <0.05 was considered statistically significant (* p <0.05, ** p <0.01, *** p <0.001, ns: no difference)

Article Snippet: The GAPDH and CD31 antibodies were procured from Proteintech (Cat No.60004-1-Ig, Cat No.11265-1-AP, Wuhan, China), while the PTGS2, SLC7A11, GPX4, and VEGFA antibodies were acquired from MedChemExpress (HY- P80091 , HY- P80935 , HY- P80450 , HY- P80929 , MCE, USA).

Techniques: Knockdown, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Fluorescence, Comparison

Journal: Antioxidants (Basel, Switzerland)

Article Title: Inhibition of Oxidative Stress and ALOX12 and NF-κB Pathways Contribute to the Protective Effect of Baicalein on Carbon Tetrachloride-Induced Acute Liver Injury.

doi: 10.3390/antiox10060976

Figure Lengend Snippet: Figure 2. Protein expression levels involved ferroptosis were detected in the liver tissues of mice challenged with CCl4. (A) Protein expression levels of ALOX12, HO-1, COX-2, p21 and Nrf2 at 6 and 24 h identified by Western blotting analysis of liver tissues of mice treated with CCl4; the quantitative analysis is showed in (B) (n = 4). 6 h vs. ctrl, * p < 0.05, ** p < 0.01; 24 h vs. ctrl, # p < 0.05, ## p < 0.01. Ctrl: control.

Article Snippet: The following primary antibodies were used to probe the membranes: primary rabbit antibodies against p21 (1:1000), COX2 (1:1000), Nrf2 (1:1000), and HO-1 (1:1000) (ProteinTech Group, Inc., Chicago, IL, USA), mouse monoclonal antibody against ALOX12 (1:1000) (Abcam, Cabridge, MA, USA) and β-actin (1:1000) (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Expressing, Western Blot, Control

Journal: Antioxidants (Basel, Switzerland)

Article Title: Inhibition of Oxidative Stress and ALOX12 and NF-κB Pathways Contribute to the Protective Effect of Baicalein on Carbon Tetrachloride-Induced Acute Liver Injury.

doi: 10.3390/antiox10060976

Figure Lengend Snippet: Figure 6. Effects of baicalein supplementation on apoptotic pathway in the liver tissues of mice challenged with CCl4. (A) Representative TUNEL-stained sections showing apoptosis in the liver tissue of mice. (B) Quantitative analysis of TUNEL positive rates (n = 4). (C,D) Activities of (C) caspases-3 and -9 (D) (n = 6). (E) The relative expression of Bax, GADD45a and p21 mRNAs in the liver tissues (n = 5). ** p < 0.01, compared to the control group; # p < 0.05 or ## p < 0.01, compared to the CCl4 only group. Bar = 100 µm. Bai: baicalein.

Article Snippet: The following primary antibodies were used to probe the membranes: primary rabbit antibodies against p21 (1:1000), COX2 (1:1000), Nrf2 (1:1000), and HO-1 (1:1000) (ProteinTech Group, Inc., Chicago, IL, USA), mouse monoclonal antibody against ALOX12 (1:1000) (Abcam, Cabridge, MA, USA) and β-actin (1:1000) (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: TUNEL Assay, Staining, Expressing, Control

Journal: Regulatory peptides

Article Title: Increased circulating angiotensin-(1-7) protects white adipose tissue against development of a proinflammatory state stimulated by a high-fat diet.

doi: 10.1016/j.regpep.2012.06.009

Figure Lengend Snippet: Fig. 3. Adipose tissue inflammatory markers. Western blot for COX2-epididymal adipose tissue (A), RT-PCR expression of IL-1β‐epididymal adipose tissue (B), SD immunohisto- chemical reaction for IL-1β in epididymal adipose tissue (C), TGR immunohistochemical reaction for IL-1β in epididymal adipose tissue (D), RT-PCR expression of TNF-α‐ epididymal adipose tissue (E), SD immunohistochemical reaction for TNF-α in epididymal adipose tissue (F), TGR immunohistochemical reaction for TNF-α in epididymal adipose tissue (G). Data are presented as mean±SEM; * pb0.05.

Article Snippet: The blot was probed with COX2 enzyme antibody (Cell Signaling, USA; 1:1000), diluted in 1× blocking buffer, washed in 1× TBS with 1% of Tween 20, and then incubated with either IRDye 800 anti-goat secondary antibody (Rockland Immunochemicals®, Gilbertsville, Pa.) diluted to 1:10,000 in blocking buffer followed by further washes with PBS-Tween 20 and one wash in TBS.

Techniques: Western Blot, Reverse Transcription Polymerase Chain Reaction, Expressing, Immunohistochemical staining

Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Antibodies

Article Snippet: MT-CO2 , Rabbit , Cell Signaling Technology , #31,219.

Techniques:

Journal: Diabetology & Metabolic Syndrome

Article Title: Didymin protects pancreatic beta cells by enhancing mitochondrial function in high-fat diet-induced impaired glucose tolerance

doi: 10.1186/s13098-023-01244-1

Figure Lengend Snippet: Didymin improves mitochondrial function in PBCs. ( A ) MitoTracker Green staining for mitochondrial content in INS-1 cells. Scale bar = 30 μm. (n = 4) ( B ) Western blot analysis of NRF1, TFAM, NDUFB8, SDHB and MTCO2 in INS-1 cells (n = 3). ( C ) Mitochondrial oxygen consumption ratio (OCR) of INS-1 cells (n = 4). ( D ) ROS concentration in INS-1 cells (n = 4). ( E ) Analysis of mitochondrial structure of PBCs in mice by electron microscopy. Scale bar = 0.6 μm. ( F ) Immunofluorescence staining of COX-IV (green) and insulin (red) in pancreas. Scale bar = 20 μm. Data are expressed as mean ± SD. * P < 0.05, ** P < 0.01, **** P < 0.0001 PA vs. PA + Didymin. # P < 0.05, ## P < 0.01, ### P < 0.001, #### P < 0.0001 control vs. PA.

Article Snippet: MT-CO2 , Rabbit , Cell Signaling Technology , #31,219.

Techniques: Staining, Western Blot, Concentration Assay, Electron Microscopy, Immunofluorescence, Control