Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Orthogonal Experimental Design and Results of CoQ10-Lip

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Trend chart of factor levels in CoQ10-Lip.

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Characterization of (E+Q)-Lip-Apt (n=3) ( \documentclass[12pt]{minimal} \usepackage{wasysym} \usepackage[substack]{amsmath} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage[mathscr]{eucal} \usepackage{mathrsfs} \DeclareFontFamily{T1}{linotext}{} \DeclareFontShape{T1}{linotext}{m}{n} {linotext }{} \DeclareSymbolFont{linotext}{T1}{linotext}{m}{n} \DeclareSymbolFontAlphabet{\mathLINOTEXT}{linotext} \begin{document}$\overline {x } \pm {\ }s$\end{document} )

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques: Zeta Potential Analyzer

Journal: International Journal of Nanomedicine

Article Title: Aptamer Functionalized Liposomes Co-Loaded with Exenatide-4 and Coenzyme Q10 Ameliorate Type 2 Diabetes Mellitus by Improving Pancreatic β Cell Function

doi: 10.2147/IJN.S510240

Figure Lengend Snippet: Factor Levels of CoQ10-Lip

Article Snippet: CoQ10 and Ext-4 were purchased from Med Chem Express (Guangzhou, China); Phosphatidylcholine (PC), cholesterol (Chol), and streptozotocin (STZ) were purchased from Aladdin Biochemical Technology (Shanghai, China).

Techniques:

Journal: Scientific Reports

Article Title: TIPE2 suppresses ferroptosis and pro-inflammatory polarization in macrophages triggered by SARS-CoV-2 spike protein

doi: 10.1038/s41598-025-14235-1

Figure Lengend Snippet: TIPE2 regulates ferroptosis-related pathways in THP-1 macrophages following SARS-CoV-2 spike protein stimulation. a , b Expression of ferroptosis-related genes in THP−1 macrophages by qRT-PCR and Western blotting. the ferroptosis-related proteins including GPx4, SLC7A11,FSP1 and ACSL4. Cropped blot is shown for clarity, retaining relevant bands. The uncropped, full-length blot with visible edges is included in Supplementary Figure S3, 4. c Cell viability was evaluated by CCK8 assay in TIPE2-transfected or control THP-1 macrophages treated with S protein alone or in combination with Fer-1 (2µM). d-h Quantification of intracellular levels of ferroptosis-associated metabolic indicators, including ferrous iron (Fe² + ), Coenzyme Q10 (CoQ10), NADP⁺/NADPH ratio, malondialdehyde (MDA), and GSH/GSSG ratio in THP-1 macrophages under the indicated treatments. i Representative fluorescence microscopy images showing lipid ROS accumulation in THP-1 macrophages labeled with specific probes. Nuclei are stained blue (Hoechst), lipid ROS appear in green (FITC channel), and merged images are presented. Data are representative of at least three independent experiments. Student’s t-test evaluated differences between two groups, and one-way ANOVA with post-hoc testing (LSD or Tamhane’s T2) was used for comparisons among three groups. (* P < 0.05, ** P < 0.01, *** P < 0.001)

Article Snippet: Intracellular coenzyme (Co) Q10 levels were determined using a human CoQ10 ELISA kit (CUSABIO, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Transfection, Control, Fluorescence, Microscopy, Labeling, Staining