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Image Search Results
Journal: PLoS ONE
Article Title: 11β-Hydroxysteroid Dehydrogenase Type 1 Gene Knockout Attenuates Atherosclerosis and In Vivo Foam Cell Formation in Hyperlipidemic apoE −/− Mice
doi: 10.1371/journal.pone.0053192
Figure Lengend Snippet: Cytokine protein levels detected in cell culture media by protein multiplex analysis following overnight exposure of peritoneal foam cells to ∼30 µg/ml of oxidized LDL (Ox-LDL) vs. untreated controls. Peritoneal foam cells were harvested from Western diet-fed 11βHSD1 +/+ /apoE −/− (+/+) and 11βHSD1 −/− /apoE −/− (−/−) mice. G-CSF, KC, MCP1 and TNF-α levels were normalized to total cellular protein content. Cell culture media contained 200 nM 11-dehydrocorticosterone throughout the course of the experiment. Significance vs. control: *p≤0.05.
Article Snippet: Macrophages were incubated overnight in the presence or absence of ∼30 µg/ml copper-oxidized
Techniques: Cell Culture, Multiplex Assay, Western Blot