control‑plasmid Search Results


irak 4 crispr cas9 knockout plasmid  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology irak 4 crispr cas9 knockout plasmid
Irak 4 Crispr Cas9 Knockout Plasmid, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid backbone  (Addgene inc)
93
Addgene inc plasmid backbone
Plasmid Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control shrna plasmid a  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology control shrna plasmid a
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Control Shrna Plasmid A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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control shrna plasmid a - by Bioz Stars, 2026-03
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plko tet on plasmid  (Addgene inc)
93
Addgene inc plko tet on plasmid
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Plko Tet On Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1  (Addgene inc)
96
Addgene inc plko 1
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Plko 1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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psnapf h2b control plasmid  (Addgene inc)
93
Addgene inc psnapf h2b control plasmid
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Psnapf H2b Control Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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non targeting control sgrnas  (Addgene inc)
94
Addgene inc non targeting control sgrnas
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Non Targeting Control Sgrnas, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl3 basic vector  (Addgene inc)
93
Addgene inc pgl3 basic vector
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Pgl3 Basic Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vector pgl2 empty control sv40 luc  (Addgene inc)
93
Addgene inc vector pgl2 empty control sv40 luc
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Vector Pgl2 Empty Control Sv40 Luc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pn3 control  (Addgene inc)
93
Addgene inc pn3 control
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Pn3 Control, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pgl3 control pten 3 utr mut 31  (Addgene inc)
85
Addgene inc pgl3 control pten 3 utr mut 31
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Pgl3 Control Pten 3 Utr Mut 31, supplied by Addgene inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plko 1 puro lentiviral vector  (Addgene inc)
95
Addgene inc plko 1 puro lentiviral vector
Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 <t>shRNA</t> (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.
Plko 1 Puro Lentiviral Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 shRNA (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.

Journal: Human molecular genetics

Article Title: Expression of tumor-promoting Cyr61 is regulated by hTRA2-β1 and acidosis.

doi: 10.1093/hmg/ddr128

Figure Lengend Snippet: Figure 4. Cyr61 alternative splicing altered by splicing factor hTRA2-beta1. (A) Expression profiles of Cyr61 mRNA isoforms in HeLa, Ishikawa, SK-OV-3 and MCF-7 cells under (C) normal conditions (control), (1) after transient transfection with hTRA2-beta1 expression plasmid (48 h), and (2) hTRA2-beta1 knock- down mediated by transient transfection with hTRA2-beta1 shRNA (SCBT; 48 h). Overexpression of hTRA2-beta1 had no influence on Cyr61 alternative spli- cing. In contrast, hTRA2-beta1 down-regulation caused a marked shift of Cyr61 alternative splicing pattern toward the expression of the intron 3-free Cyr61 mRNA isoform (481 bp). Expression of 18 S RNA served as a comparative value. RT–PCR, triplicate experiments. (B) The efficacy of hTRA2-beta1 knock- down and overexpression experiments was proven by detection of significant decrease or increase in the hTRA2-beta1 protein. hTRA2-beta1 protein expression under (2) shRNA-mediated hTra2-beta1 (SCBT) knock-down, (+) hTRA2-beta1 overexpression induced by transient transfection with hTRA2-beta1 expression plasmid (courtesy of S. Stamm), and as a control (C) under regular conditions and in cancer cell lines HeLa, Ishikawa, SK-OV-3 and MCF-7. Immu- nostaining of the hTRA2-beta1 protein with rabbit IgG primary hTRA2-beta1 Ab (courtesy of S. Stamm), and COX IV protein expression as comparative value, immunostained with COX IV mAb (#3E11, Cell Signalling). Western blot. Dashed lines indicate origin from different gels.

Article Snippet: For transient transfections, cells were plated at density of 5 × 105 cells per 10 cm2 flask right before transfection. hTRA2-beta1 expression plasmid as well as TRA2-beta1 shRNA plasmid and control shRNA plasmid A [Santa Cruz Biotechnology, Inc. (SCBT)] were transfected with SatisFectionTM Transfection Reagent (STRATAGENEw, Agilent Technologies) following the manufacturer’s protocol.

Techniques: Alternative Splicing, Expressing, Control, Transfection, Plasmid Preparation, Knockdown, shRNA, Over Expression, Reverse Transcription Polymerase Chain Reaction, Western Blot