Journal: Blood

Article Title: Effects of intravenous immunoglobulin on platelet count and antiplatelet antibody disposition in a rat model of immune thrombocytopenia

doi: 10.1182/blood.v100.6.2087

Figure Lengend Snippet: Figure 3. IVIG does not directly bind 7E3. 7E3 (f) (or control IgG, ) and IVIG were combined in vitro, at a constant IVIG concentration (25 mg/mL) and varying 7E3 concentrations (0-0.1 mg/mL). The positive control was a mouse antihuman IgG. Samples were then added to a microplate coated with antihuman IgG. Murine IgG binding was visualized using a secondary antimouse IgG–alkaline phosphatase conjugate. p-Nitrophenyl phosphate was added, and the plates were read at 405 nm (kinetic assay, over 10 minutes). Assay response to 7E3 did not differ from control (P .164), whereas the positive control differed significantly from control (P .001).

Article Snippet: Goat antihuman IgG (no cross-reactivity to goat and mouse serum proteins) and alkaline phosphatase–conjugated goat antimouse IgG (no cross-reactivity to goat and human serum proteins) were both obtained from Rockland (Gilbertsville, PA).

Techniques: Control, In Vitro, Concentration Assay, Positive Control, Binding Assay, Kinetic Assay

Journal: Oncotarget

Article Title: Host genotype and tumor phenotype of chemokine decoy receptors integrally affect breast cancer relapse.

doi: 10.18632/oncotarget.4470

Figure Lengend Snippet: Figure 2: Tumor expression of chemokine decoy receptors (CDR) and host genotype of CDR jointly affect breast cancer relapse. A. Effect of tumor phenotype of CDR on relapse-free survival (RFS). P for log rank = 7.5 × 10−6. The RFS curve was derived from the Kaplan-Meier estimate, and the survival differences between groups were compared by log-rank test. B. Effect of host genotype of CDR on RFS. P for log rank = 0.002 C. Joint effect of tumor phenotype and host genotype of CDR on RFS. P-values of the differences between high expression/minor genotype group and high expression/major genotype group, high expression/major genotype group and low expression/ minor genotype group, and low expression/minor genotype group and low expression/major genotype group are 0.007, 0.354, and 0.047, respectively. High expression indicates co-expression of DARC and D6, otherwise low expression. Minor genotype indicates patients with at- least-one protective minor allele, otherwise major genotype. D. Chemokine levels in the supernatant of cells detected by ELISA after 24-hour incubation. For transient transfection, 1 μg pDARC-42G or -42A, 1 μg pD6-373S or -373Y, or the combination of 1 μg variant-type DARC- 42A and 1 μg variant-type pD6-373Y were transfected. An empty expression vector was also used as a control. 72 hours after transfection, the levels of human CCL2 and CCL5 in cell supernatants were determined with a sandwich ELISA. Columns represent the mean of three independent experiments; bars, standard error; *, P < 0.05. E. ROC curves assessing the discriminatory performance of the CDR phenotype/ genotype model and the CDR phenotype model for the prediction of disease relapse. P = 0.02 for AUC comparison.

Article Snippet: After 72 hours of transfection, the levels of human CCL2 and CCL5 in cell supernatants were determined with a sandwich ELISA (R&D systems, USA).

Techniques: Expressing, Derivative Assay, Enzyme-linked Immunosorbent Assay, Incubation, Transfection, Variant Assay, Plasmid Preparation, Control, Sandwich ELISA, Comparison