Journal: Small (Weinheim an der Bergstrasse, Germany)

Article Title: Cellular association and assembly of a multistage delivery system.

doi: 10.1002/smll.201000126

Figure Lengend Snippet: Figure 7. Endothelial targeting with the assembled MDSs. A) HUVECs were incubated with either control IgG fluorescein-labeled MDSs (A) or anti- VEGFR-2 fluorescein antibody-labeled MDSs (B,C) for 2 h at 37 8C (10:1 ratio of particles to cells). C) Antibody-targeted cells are shown at two magnifications,with3Dimagesatthecrosshairsofthehighermagnificationimage(middle).Totheright,lamellopodiaprojectingfromtwocellsmake contactwiththeMDS(63oilimmersionlens;A,B)bar25 mm,C) bars50and7.5 mm;actin:rhodaminephalloidin;nuclei:DRAQ5).D)Flowcytometry dot plot (left) showing microparticle light scatter and a histogram (right) showing microparticle fluorescence before and after conjugation with fluorescentantibody. E) Bar graph (top) showingthe relative association of the MDSswith HUVEC andHMVEC endothelial cells conjugated with either control IgG or anti-PECAM-specific antibody. Below are representative flow cytometry dot plots showing the increase in side scatter in HUVECs after incubation with targeted (anti-PECAM) MDSs.

Article Snippet: Antibody conjugation: Anti-PECAM antibody was obtained from Sigma, while anti-VEGF R2/KDR (vascular endothelial growth factor receptor) and isotype control (IgG1) fluorescein-conjugated antibodies were purchased from R&D Systems (Minneapolis, MN).

Techniques: Incubation, Control, Labeling, Conjugation Assay, Cytometry