Journal: Frontiers in Immunology

Article Title: Serological cross-reactivity between Crimean-Congo haemorrhagic fever virus and Nairobi sheep disease virus glycoprotein C

doi: 10.3389/fimmu.2024.1423474

Figure Lengend Snippet: Orthonairovirus test antigens employed during experiments.

Article Snippet: CCHFV Gc , NP_950235 (IbAr10200, Nigeria) , HEK293 (Human) , The Native Antigen Company, UK #REC31696.

Techniques: Expressing, Plasmid Preparation

Journal: Viruses

Article Title: Generation and Characterisation of Monoclonal Antibodies against Nairobi Sheep Disease Virus Nucleoprotein.

doi: 10.3390/v15091876

Figure Lengend Snippet: Figure 2. Monoclonal antibodies generated react to NSDV but neither CCHFV nor DUGV NP. GG12 and AF5 were assessed for their ability to bind to NSDV NP but not CCHFV and DUGV NP. (a) ELISA performed using either GG12 (black) or AF5 (grey) at 1:100–1:2500 dilution (1 mg/mL stock concentration) on plates coated with 0.50 ng/well of NSDV, CCHFV or DUGV NP. (b) Western blot using GG12 at 1:2000 dilution on membranes containing NSDV (first lane), DUGV (middle lane), and CCHFV (right lane). Ponceau staining was performed to confirm successful transfer of all NPs onto the membrane.

Article Snippet: Recombinant NSDV NP (Accession number: YP_009361831.1) was produced and provided by Reading University; DUGV NP (Accession number: Q8V336; Cat # CSBEP851800DCAH-CSB) was from Stratech; and CCHFV NP (Accession number: NP_950237; Cat# REC31639) was from the Native Antigen Company, Oxford.

Techniques: Bioprocessing, Generated, Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot, Staining, Membrane