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Danaher Inc
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Danaher Inc
imagexpress micro system Imagexpress Micro System, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/confocal+imaging/pmc04729552-150-6-9?v=Danaher+Inc Average 96 stars, based on 1 article reviews
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Olympus
ixplore spin confocal imaging microscope Ixplore Spin Confocal Imaging Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/confocal+imaging/pm41688788-189-5-10?v=Olympus Average 96 stars, based on 1 article reviews
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Revvity
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Nikon
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heidelberg engineering
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Curiochips inc
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Nanonics Imaging Ltd
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Hamamatsu
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Becton Dickinson
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3i - Intelligent Imaging
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3i - Intelligent Imaging
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Image Search Results
Journal: Gastro Hep Advances
Article Title: A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids
doi: 10.1016/j.gastha.2024.08.004
Figure Lengend Snippet: Twenty-eight-day Curiochip HLO viability. (A) HLOs were plated on Curiochips, and output media was periodically assessed for albumin secretion (n = 16). (B) CYP450 gene expression was quantified from parallel time points after cell lysis (n = 3). Data were compared to day 14 Curiochips. (C) Day 7 Curiochip cultures were assessed for metabolic function by quantifying molecular turnover of acetaminophen (APAP) and darunavir (DRV) exclusively metabolized by CYP450s using LC/MS/MS; n = 3 and 16 for HLO and Curiochips, respectively. Data are expressed as mean ± standard error of the mean. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001; n.s., not significant.
Article Snippet: In situ differentiation of
Techniques: Gene Expression, Lysis, Liquid Chromatography with Mass Spectroscopy
Journal: Gastro Hep Advances
Article Title: A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids
doi: 10.1016/j.gastha.2024.08.004
Figure Lengend Snippet: Functional and phenotypic assessment of Curiochips as a long-term DILI model. (A) Schematic drawing of chronic DILI assay using HLOs on Curiochips. Curiochips were treated for 7 days with either (B) APAP or (C) FIAU and assessed for kinetic hepatocyte function and injury through biochemical analysis of albumin secretion and ALT activity. Multiple comparisons were made between sequential drug treatment time points. (D) Fluorescent images of vehicle control and drug-treated Curiochips stained for DNA and neutral lipid droplets were (E) quantified for total lipid intensity. Data are expressed as mean ± SEM of n = 16 Curiochips per treatment group. ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001; n.s., not significant.
Article Snippet: In situ differentiation of
Techniques: Functional Assay, Activity Assay, Control, Staining
Figure 2 ). (C) CYP450 gene expression (n = 3) from in situ differentiated Curiochips compared to ex situ differentiation, both at day 21 of differentiation. DILI model evaluation of in situ Curiochips was quantified by ALT activity after (D) APAP and (E) FIAU treatments, over (n = 16 for each condition). Data are expressed as mean ± SEM; ∗ P < .05; ∗∗ P < .01; ∗∗∗ P < .001; ∗∗∗∗ P < .0001; n.s., not significant. " width="100%" height="100%">
Journal: Gastro Hep Advances
Article Title: A High-Throughput Microphysiological Liver Chip System to Model Drug-Induced Liver Injury Using Human Liver Organoids
doi: 10.1016/j.gastha.2024.08.004
Figure Lengend Snippet: In situ differentiation of HLOs on Curiochips. (A) Confocal fluorescence imaging of HLOs differentiated directly on Curiochips with cell-specific staining of hepatocytes (CEBPA) and hepatic stellate cells (vimentin). (B) Kinetic albumin secretion from in situ and ex situ differentiation of HLOs (n = 16). Multiple comparisons were made between sequential days of culture. The yellow dotted line represents the date of complete HLO differentiation (corresponds to day 0 in
Article Snippet: In situ differentiation of
Techniques: In Situ, Fluorescence, Imaging, Staining, Ex Situ, Gene Expression, Activity Assay