confocal fluorescence microscopy Search Results


ti2 e confocal laser fluorescence microscopy  (Nikon)
99
Nikon ti2 e confocal laser fluorescence microscopy
Ti2 E Confocal Laser Fluorescence Microscopy, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pm36515759-51-8-7?v=Nikon
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ti2 e confocal laser fluorescence microscopy - by Bioz Stars, 2026-08
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fluorescence confocal microscopy  (CrestOptics)
90
CrestOptics fluorescence confocal microscopy
Fluorescence Confocal Microscopy, supplied by CrestOptics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pmc11919828-109-15-18?v=CrestOptics
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fluorescence confocal microscopy - by Bioz Stars, 2026-08
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time-lapse fluorescence laser confocal microscopy  (KEYENCE)
90
KEYENCE time-lapse fluorescence laser confocal microscopy
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Time Lapse Fluorescence Laser Confocal Microscopy, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pmc05214546-364-11-16?v=KEYENCE
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time-lapse fluorescence laser confocal microscopy - by Bioz Stars, 2026-08
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highthroughput confocal microscopy for beta-arrestin-green fluorescent protein translocation g protein-coupled receptor assays  (Evotec Inc)
90
Evotec Inc highthroughput confocal microscopy for beta-arrestin-green fluorescent protein translocation g protein-coupled receptor assays
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Highthroughput Confocal Microscopy For Beta Arrestin Green Fluorescent Protein Translocation G Protein Coupled Receptor Assays, supplied by Evotec Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/10__1080_slash_19768354__2009__9647199-142-14-24?v=Evotec+Inc
Average 90 stars, based on 1 article reviews
highthroughput confocal microscopy for beta-arrestin-green fluorescent protein translocation g protein-coupled receptor assays - by Bioz Stars, 2026-08
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spinning disk system for fast fluorescence confocal microscopy  (CrestOptics)
90
CrestOptics spinning disk system for fast fluorescence confocal microscopy
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Spinning Disk System For Fast Fluorescence Confocal Microscopy, supplied by CrestOptics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pmc09965010-85-19-29?v=CrestOptics
Average 90 stars, based on 1 article reviews
spinning disk system for fast fluorescence confocal microscopy - by Bioz Stars, 2026-08
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dic, fluorescence, confocal and 2-photon microscopy images acquired with hamamatsu hcimage 4.6.0  (Hamamatsu)
90
Hamamatsu dic, fluorescence, confocal and 2-photon microscopy images acquired with hamamatsu hcimage 4.6.0
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Dic, Fluorescence, Confocal And 2 Photon Microscopy Images Acquired With Hamamatsu Hcimage 4.6.0, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/10__1038_slash_s41566___020___0631___z-293-8-21?v=Hamamatsu
Average 90 stars, based on 1 article reviews
dic, fluorescence, confocal and 2-photon microscopy images acquired with hamamatsu hcimage 4.6.0 - by Bioz Stars, 2026-08
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confocal fluorescence microscopy netgra spectra  (NT MDT America Inc)
90
NT MDT America Inc confocal fluorescence microscopy netgra spectra
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Confocal Fluorescence Microscopy Netgra Spectra, supplied by NT MDT America Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/10__1007_slash_s12274___013___0296___8-30-5-10?v=NT+MDT+America+Inc
Average 90 stars, based on 1 article reviews
confocal fluorescence microscopy netgra spectra - by Bioz Stars, 2026-08
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confocal fluorescence microscopy  (Khushi Inc)
90
Khushi Inc confocal fluorescence microscopy
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Confocal Fluorescence Microscopy, supplied by Khushi Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pm35377060-2753-8-18?v=Khushi+Inc
Average 90 stars, based on 1 article reviews
confocal fluorescence microscopy - by Bioz Stars, 2026-08
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confocal fluorescence microscopy images  (CEM Corporation)
90
CEM Corporation confocal fluorescence microscopy images
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Confocal Fluorescence Microscopy Images, supplied by CEM Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pmc05476801__ncomms13580___s1-5-13-18?v=CEM+Corporation
Average 90 stars, based on 1 article reviews
confocal fluorescence microscopy images - by Bioz Stars, 2026-08
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c-trap instrument brightfield imaging, 488 fluorescent confocal microscopy, dual-trap capabilities  (Lumicks B.V)
90
Lumicks B.V c-trap instrument brightfield imaging, 488 fluorescent confocal microscopy, dual-trap capabilities
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
C Trap Instrument Brightfield Imaging, 488 Fluorescent Confocal Microscopy, Dual Trap Capabilities, supplied by Lumicks B.V, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/bio_rxiv__2024__11__15__623768-230-15-1?v=Lumicks+B.V
Average 90 stars, based on 1 article reviews
c-trap instrument brightfield imaging, 488 fluorescent confocal microscopy, dual-trap capabilities - by Bioz Stars, 2026-08
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thioflavin t (tht) confocal fluorescence microscopy gel  (ibidi GmbH)
90
ibidi GmbH thioflavin t (tht) confocal fluorescence microscopy gel
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Thioflavin T (Tht) Confocal Fluorescence Microscopy Gel, supplied by ibidi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/pm32262869-56-0-29?v=ibidi+GmbH
Average 90 stars, based on 1 article reviews
thioflavin t (tht) confocal fluorescence microscopy gel - by Bioz Stars, 2026-08
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three-color confocal fluorescence microscopy  (Lumicks B.V)
90
Lumicks B.V three-color confocal fluorescence microscopy
(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by <t>fluorescence</t> <t>microscopy</t> (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).
Three Color Confocal Fluorescence Microscopy, supplied by Lumicks B.V, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/confocal+fluorescence+microscopy/bio_rxiv__2024__03__28__587287-53-9-17?v=Lumicks+B.V
Average 90 stars, based on 1 article reviews
three-color confocal fluorescence microscopy - by Bioz Stars, 2026-08
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Image Search Results


(A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).

Journal: JCI Insight

Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

doi: 10.1172/jci.insight.86492

Figure Lengend Snippet: (A) Day 50–60 organoids from human embryonic stem cells (hESCs) (SEES1 cells) immunostained with markers for intestinal differentiation: villin, leucine-rich repeat containing G protein–coupled receptor 5 (LGR5), CDX2, E-cadherin (ECAD), chromogranin A (CGA), mucin-2 (MUC2), defensin α-6, Paneth cell–specific (DEFA6), α-smooth muscle actin (SMA), and protein gene product 9.5 (PGP9.5). Cell nuclei were counterstained with DAPI. PGP9.5-positive enteric neuronal cells within the α-SMA–positive myenteric area are indicated by yellow arrowheads. Scale bar: 50 μm (VILLIN, LGR5, SMA); 100 μm (ECAD and MUC2). (B) An enterocyte with a characteristic brush border (left), and Paneth cells with secretory granules (black arrowhead) and goblet cells containing mucin granules (yellow arrowhead) (right). Scale bar: 10 μm (left); 5 μm (right). (C) Organoids that developed from SEES1 cells expressed EGFP under the LGR5 promoter (green), indicating that they were LGR5-positive gut organoids. Gut tube-like architecture in a day 34 organoid (red square; high-magnification view, white square) (top row). A small number of EGFP-positive cells were detected in the day 34 organoid (white square) by fluorescence microscopy (bottom left). The number of EGFP-positive cells increased at day 41 (bottom right). Scale bar: 300 μm (top left), 100 μm (top right and bottom row).

Article Snippet: Five μM forskolin was added, and organoid morphology was monitored by time-lapse fluorescence laser confocal microscopy (Keyence).

Techniques: Fluorescence, Microscopy

(A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).

Journal: JCI Insight

Article Title: A xenogeneic-free system generating functional human gut organoids from pluripotent stem cells

doi: 10.1172/jci.insight.86492

Figure Lengend Snippet: (A) Day 115 gut organoids from hESCs (SEES1 cells) immunostained with markers for the cystic fibrosis conductance regulator (CFTR). CFTR was present in the epithelial layers of the gut organoids as well as in the control human intestine. CFTR (green), actin (red), and DAPI (blue). Scale bar: 100 μm. (B) Quantification of forskolin-induced gut organoid swelling. Gut organoids (green) derived from SEES1 cells were monitored by time-lapse fluorescence laser confocal microscopy (Keyence). The organoid surface area (red) was quantified using Hybrid Cell Count BZ-H3C (Keyence). The normalized total organoid surface area was calculated and averaged from 3 individual wells per treatment (n = 3 for each group). A forskolin-induced organoid (19 minutes) was overlaid with a pretreated organoid (0 minutes; 569 μm).

Article Snippet: Five μM forskolin was added, and organoid morphology was monitored by time-lapse fluorescence laser confocal microscopy (Keyence).

Techniques: Control, Derivative Assay, Fluorescence, Confocal Microscopy, Cell Counting