Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Mean plasma pantoprazole concentration (logarithmic scale) vs. time (hr) profiles for neonatal calves ( n = 9) following intravenous (IV) single dose administration of 1.0 mg/kg of pantoprazole.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques: Clinical Proteomics, Concentration Assay

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Pantoprazole pharmacokinetic parameters following a single intravenous (1 mg/kg) administration to neonatal Holstein calves.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Tissue concentrations of pantoprazole sulfone (μg/g) in collected tissues 1, 3, and 5 days after intravenous administration of pantoprazole (1 mg/kg) from study calves.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Pharmacokinetics and Tissue Levels of Pantoprazole in Neonatal Calves After Intravenous Administration

doi: 10.3389/fvets.2020.580735

Figure Lengend Snippet: Comparisons of pharmacokinetic parameters of pantoprazole in domestic animal species, after single dose intravenous administration.

Article Snippet: Pantoprazole sulfone (Toronto Research Chemicals, Ontario, Canada) became the analyte spiked into blank tissue samples to generate calibration spikes and QC samples.

Techniques:

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: (a) Body mass at baseline (week 0; striped) and the conclusion (solid) of the 8-week intervention [n=15-19/group]. (b) Plasma ALT [n=9-12/group], (c) plasma AST [n=6-14/group], (d) liver mass [n=15-19/group], (e) liver TG [n=11-17/group], and (f) liver pathological NASH Activity and Brunt Fibrosis Scoring at the conclusion of the 8-week intervention [n=10-14/group]. (g) Representative images of gross morphology, H&E stained (magnification = 20X; scale bar = 50 μm), and Masson’s trichrome stained (magnification = 20X; scale bar = 50 μm) liver at the conclusion of the 8-week intervention. All data are shown as mean ± SEM and were analyzed by paired Student’s t-test within treatment group (a) or one-way ANOVA with Tukey post-hoc testing (b-f). We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, **p< 0.01, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques: Clinical Proteomics, Activity Assay, Staining

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: (a) Liver TGF-β1 at the conclusion of the 8-week intervention [n=13-17/group]. (b) Collagen synthesis rates during the 8-week intervention [n=14-18/group]. (c) Liver TGF-β1 hydroxyproline content at the conclusion of the 8-week intervention [n=14-19/group]. (d) Fractional percent [n=15-18/group], and (e) absolute content [n=15-18/group] of static and dynamic collagen in liver at the conclusion of the 8-week intervention. All data are shown as mean ± SEM and were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, **p< 0.01, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques:

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: Liver (a) Loxl2 mRNA [n=9-12/group], and (b) LOXL2 protein [n=14/group] at the conclusion of the 8-week intervention. (c) Representative immunoblots of liver LOXL2 and GAPDH at the conclusion of the 8-week intervention. All data are shown as mean ± SEM and were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques: Western Blot

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: Liver (a) Mmp2 mRNA [n=9-12/group], (b) Mmp9 mRNA [n=9-12/group], (c) Timp1 mRNA [n=8-11/group], (d) MMP2 protein [n=12-17/group], (e) MMP9 protein [n=12-17/group], and (f) TIMP1 protein [n=12-17/group] at the conclusion of the 8-week intervention. (g) Representative immunoblots of liver MMP2, MMP9, TIMP1, and GAPDH at the conclusion of the 8-week intervention. Liver (h) MMP2 [n=10-14/group], and (i) MMP9 [n=11-14/group] enzymatic activity at the conclusion of the 8-week intervention. (j) Representative images of liver gelatin zymography assessing MMP2 and MMP9 enzymatic activity. Signal intensity for each sample was normalized to the intensity of their corresponding positive control standard. All data are shown as mean ± SEM and were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, **p< 0.01, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques: Western Blot, Activity Assay, Zymography, Positive Control

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: Liver (a) Mmp8 mRNA [n=8-12/group], (b) Mmp12 mRNA [n=9-12/group], (c) Mmp13 mRNA [n=9-12/group], (d) Mmp14 mRNA [n=9-12/group], (e) Mmp16 mRNA [n=9-12/group], and (f) Mmp19 mRNA [n=9-12/group] at the conclusion of the 8-week intervention. All data are shown as mean ± SEM and were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. **p< 0.01, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques:

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: Liver (a) Pparγ mRNA [n=9-12/group], (b) Scd1 mRNA [n=9-12/group], (c) PPARγ protein [n=12-17/group], and (d) SCD1 protein [n=8-14/group] at the conclusion of the 8-week intervention. (e) Representative immunoblots of liver PPARγ, SCD1, and GAPDH at the conclusion of the 8-week intervention. All data are shown as mean ± SEM and were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, **p< 0.01, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques: Western Blot

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: (a) Liver fetuin-A protein at the conclusion of the 8-week intervention [n=12-17/group]. (b) Representative immunoblots of liver fetuin-A and GAPDH at the conclusion of the 8-week intervention. All data are shown as mean ± SEM and were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, **p< 0.01.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques: Western Blot

Journal: bioRxiv

Article Title: 17α-estradiol, a lifespan-extending compound, attenuates liver fibrosis by modulating collagen turnover rates in male mice

doi: 10.1101/2022.06.16.496423

Figure Lengend Snippet: (a) Representative immunofluorescence images of F4/80 (total macrophages), CD11c (M1: pro-inflammatory macrophages), and CD206 (M2: anti-inflammatory macrophages) in liver at the conclusion of the 8-week intervention (magnification = 320X; scale bar = 50 μm). Percentage of liver area for (b) F4/80 [n=5-7/group], (c) CD11c [n=5-7/group], and (d) CD206 [n=5-7/group] at the conclusion of the 8-week intervention. (e) Ratio of liver phospho to total p65 at the conclusion of the 8-week intervention [n=12-16/group]. (f) Representative immunoblots of liver phosphor p65, total p65, and GAPDH at the conclusion of the 8-week intervention. All data are shown as mean ± SEM were analyzed by one-way ANOVA with Tukey post-hoc testing. We did not indicate statistical differences between vehicle and 17α-E2 treatment groups (preventive and therapeutic), or between 17α-E2 treatment groups (preventive and therapeutic), for purposes of visual clarity. *p<0.05, ***p<0.005.

Article Snippet: At twelve weeks of age, mice were randomized by body mass into one of five groups , (1) Short-term carbon tetrachloride (CCl 4 ) (n=18): chow-fed (TestDiet 58YP; 66.6% CHO, 20.4% PRO, 13.0% FAT), CCl 4 -treated twice weekly for four weeks, (2) Vehicle (n=15): chow-fed, vehicle-treated (olive oil) controls, (3) CCl 4 (n=15): chow-fed, CCl 4 -treated twice weekly for eight weeks, (4) CCl 4 + 17α preventive (n=18): chow+17α (14.4 mg/kg; Steraloids, Newport, RI)-fed for eight weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks, or (5) CCl 4 + 17α therapeutic (n=15): chow+17α-fed for the final four weeks while simultaneously being CCl 4 -treated twice weekly for eight weeks.

Techniques: Immunofluorescence, Western Blot

Journal: International Journal of Biological Sciences

Article Title: Ebastine exerts antitumor activity and induces autophagy by activating AMPK/ULK1 signaling in an IPMK-dependent manner in osteosarcoma

doi: 10.7150/ijbs.69541

Figure Lengend Snippet: High-throughput screening generates candidate anti-inflammatory agents for osteosarcoma therapy. ( a ) The composition of this immunology inflammation screening library (L4100). ( b-d ) The inhibition rate of every compound in all three osteosarcoma cell lines. ( e ) The inhibition rate > 95% in three osteosarcoma cell lines was achieved by ebastine.

Article Snippet: The immunology inflammation screening library (L4100), dorsomorphin (S7840) and 3-methyladenine (3-MA, S2767) were purchased from Selleck (Shanghai, China).

Techniques: High Throughput Screening Assay, Inhibition