Journal: American Journal of Clinical and Experimental Immunology

Article Title: Role of cytokines, chemokines, C3a, and mannose-binding lectin in the evolution of the chikungunya infection

doi:

Figure Lengend Snippet: Comparison of MBL, C3a and cytokines levels in patient with CHIKV infection and healthy controls in acute and chronic phases n = 83

Article Snippet: C3a and MBL levels were measured using a Human Complement C3a ELISA Kit (Novus biologicals ® - Biotechne ® , Canada) and a Quantikine ® ELISA Human MBL Immunoassay kit (R & D Systems ® - biotechne ® , USA), respectively.

Techniques: Comparison, Infection

Journal: American Journal of Clinical and Experimental Immunology

Article Title: Role of cytokines, chemokines, C3a, and mannose-binding lectin in the evolution of the chikungunya infection

doi:

Figure Lengend Snippet: Comparison of MBL, C3a and cytokines levels in patient with CHIKV infection according to presence/persistence of musculoskeletal disorder in acute and chronic phases

Article Snippet: C3a and MBL levels were measured using a Human Complement C3a ELISA Kit (Novus biologicals ® - Biotechne ® , Canada) and a Quantikine ® ELISA Human MBL Immunoassay kit (R & D Systems ® - biotechne ® , USA), respectively.

Techniques: Comparison, Infection

Journal: Experimental and Therapeutic Medicine

Article Title: The complement C3a-C3aR and C5a-C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF-κB signaling

doi: 10.3892/etm.2022.11420

Figure Lengend Snippet: Complement C3a and C5a aggravate inflammation in HRPE cells via the NF-κB signaling pathway. HRPE cells were treated with recombinant human complement component C3a (2 µg/ml) or C5a (1 µg/ml) with or without NF-κB inhibitor PDTC (10 µM), and the release of (A) TNF-α, (B) IL-1β, (C) IL-6, (D) PGE2 and (E) IL-10 was measured by ELISA. *** P<0.001 relative to control; ### P<0.001 relative to C3a or C5a treatment. PGE2, prostaglandin E2; HRPE, human retinal pigment epithelium.

Article Snippet: Following 24 h of treatment, the contents of various cytokines and other compounds were analyzed in HRPE cell supernatants and/or human vitreous humor were determined using the following ELISA kits in accordance with the manufacturers' protocols: Tumor Necrosis Factor-α Assay Kit (cat. no. H052-1), Interleukin-1β Assay Kit (cat. no. H002), Interleukin-6 Assay Kit (cat. no. H007-1-1) and Interleukin-10 Assay Kit (cat. no. H009-1; all from Nanjing Jiancheng Bioengineering Institute); prostaglandin E2 (PGE2) ELISA Kit (cat. no. E-EL-0034c; Elabscience Biotechnology Co., Ltd.); Human Complement Fragment 3a ELISA Kit (cat no. CSB-E08509h) and Human Complement Fragment 5a ELISA Kit (cat. no. CSB-E08512h; both from Cusabio).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: The complement C3a-C3aR and C5a-C5aR pathways promote viability and inflammation of human retinal pigment epithelium cells by targeting NF-κB signaling

doi: 10.3892/etm.2022.11420

Figure Lengend Snippet: Complement C3aR and C5aR antagonist inhibit inflammation and NF-κB signaling in HRPE cells challenged with complement C3a and C5a. HRPE cells were treated with recombinant human complement component C3a (2 µg/ml) and with or without C3aR antagonist SB290157 (20 µM), and the release of (A) TNF-α, (B) IL-1β, (C) IL-6, (D) PGE2 and (E) IL-10 was determined by ELISA. HRPE cells were treated with recombinant human complement component C5a (1 µg/ml) and with or without C5aR antagonist CCX168 (2 µM), and the release of (F) TNF-α, (G) IL-1β, (H) IL-6, (I) PGE2 and (J) IL-10 was determined by ELISA. (K) The phosphorylation of NF-κB and expression of NF-κB in HRPE cells treated with recombinant human complement component C3a (2 µg/ml) and with or without C3aR antagonist SB290157 (20 µM) were determined by western blot. (L) The phosphorylation of NF-κB and expression of NF-κB in HRPE cells treated with recombinant human complement component C5a (1 µg/ml) and with or without C5aR antagonist CCX168 (2 µM) were determined by western blot. *** P<0.001 relative to control; ### P<0.001 relative to C3a or C5a treatment. p-NF-κB, phosphorylated NF-κB; C5aR, C5a receptor; HRPE, human retinal pigment epithelium; CCX, CCX168; SB, SB290157.

Article Snippet: Following 24 h of treatment, the contents of various cytokines and other compounds were analyzed in HRPE cell supernatants and/or human vitreous humor were determined using the following ELISA kits in accordance with the manufacturers' protocols: Tumor Necrosis Factor-α Assay Kit (cat. no. H052-1), Interleukin-1β Assay Kit (cat. no. H002), Interleukin-6 Assay Kit (cat. no. H007-1-1) and Interleukin-10 Assay Kit (cat. no. H009-1; all from Nanjing Jiancheng Bioengineering Institute); prostaglandin E2 (PGE2) ELISA Kit (cat. no. E-EL-0034c; Elabscience Biotechnology Co., Ltd.); Human Complement Fragment 3a ELISA Kit (cat no. CSB-E08509h) and Human Complement Fragment 5a ELISA Kit (cat. no. CSB-E08512h; both from Cusabio).

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Phospho-proteomics, Expressing, Western Blot, Control

Journal: bioRxiv

Article Title: Engineering sialylation in pigs: A promising strategy for overcoming xenograft rejection and revolutionizing organ transplantation

doi: 10.1101/2025.04.30.651299

Figure Lengend Snippet: Schematic illustration of the in-vitro experimental procedure, measurements of human thrombin/anti-thrombin III complex (TAT), C3a, C4b, and C5b9 were taken from plasma in mixed human blood and porcine kidney and aorta tissues transfected with ST8Sia6. These were compared to controls porcine kidney and aorta endothelium transfected with GFP under in vitro conditions at three different time points (0, 3, and 6 hours). Result indicates that control GFP transfected Kidney and Aorta group (grey line) had increased complement activation. b-c, indicate C3a level in kidney and aorta condition respectively, similarly d-e, represent C4b level, f-g, represent C5b9 level from kidney and aorta experimental conditions. h-i, TAT level in plasma from kidney and aorta experimental conditions. In contrast, the ST8Sia6-transfected porcine kidney group (red line) showed a slight reduction in complement activation. Notably, the ST8Sia6- transfected aorta cells demonstrated significant reductions in complement activations. All graphs summarize the results from 4 donors (mean ± S.E) with 4 individual replicates. Significance differences in P value (P<0.05) were evaluated using ANOVA, and the P values are represented in the graphs.

Article Snippet: Plasma levels of thrombin/anti-thrombin III complex (TAT), C3a, C4d, and sC5b-9 were measured using commercially available ELISA kits: Human Thrombin-Antithrombin Complex ELISA Kit (Abcam AB1089071), Human C4b (Novus Biologicals™ NBP270046), Human C3a (Novus Biologicals™ NBP266755), and Human C5b-9 (Novus Biologicals™ NBP266708).

Techniques: In Vitro, Clinical Proteomics, Transfection, Control, Activation Assay