Journal: Scientific Reports

Article Title: Anticancer activity of lactoferrin-coated biosynthesized selenium nanoparticles for combating different human cancer cells via mediating apoptotic effects

doi: 10.1038/s41598-023-36492-8

Figure Lengend Snippet: Purification of bovine lactoferrin (bLF). ( A ) Typical elution profile of bLF on CM-Sephadex C50 column. The lactoferrin (●▬●) was eluted at a salt gradient of 0.2–0.6 M NaCl ( ▬ ). All eluted fractions were immunoassayed for bLF confirmation (▲▬▲), and bLF was eluted in the first and second peaks. ( B ) 12% SDS-PAGE for bLF during purification steps. Lane 1 is protein marker, lane 2 is the raw bovine whey, lane 3 is bLF eluted from CM Sephadex C50 column and lane 4 is purified bLF eluted from Sephacryl S200 column.

Article Snippet: At first, bovine whey was applied into the pre-equilibrated CM Sephadex C50 (GE Health care, Sweden) cation exchange column and fractions containing bLF were eluted using 50 mM Tris HCl, pH of 8.0 containing a NaCl gradient of 0.0 to 1.0 M. After that, the contained bLF fractions were applied into a Sephacryl S100 column (5 × 150 mm, GE Healthcare, Sweden), which pre-equilibrated with 50 mM Tris HCl buffer, pH 7.6 and eluted with the same buffer containing 150 mM NaCl.

Techniques: Purification, SDS Page, Marker