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Image Search Results
Journal: Cell reports
Article Title: The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3 + Regulatory T Cells.
doi: 10.1016/j.celrep.2019.11.089
Figure Lengend Snippet: Figure 3. MAZR Affects Thymic Treg Cell Development (A) Dot plots depict CD25 and FOXP3 expression in WT and MAZR-cKO CD4SP thymocytes. Diagram (right) shows the percentages of CD25–FOXP3–, CD25–FOXP3+, and CD25+FOXP3+ cells, gated on CD4SP thymocytes, in WT and MAZR-cKO mice. (B) Flow cytometry analysis shows MAZR expression in the indicated T cell subsets. The colored histograms show cells pre-gated on the four WT CD25 and FOXP3 quadrants from (A), depicting four different stages of tTreg differentiation. (C) Expression of MAZR was assessed by flow cytometry in DN, DP, CD8SP, CD4SP, CD4+CD25–FOXP3+ (Treg precursors), and CD4+CD25+FOXP3+ (Treg) subsets in the thymus as well as splenic CD8+CD44–CD62Lhi (naive CD8), CD8+CD44+CD62L– (effector CD8), CD4+CD44–CD62Lhi (naive CD4), CD4+CD44+CD62L– (effector CD4), and CD4+FOXP3+ (Treg) subsets.
Article Snippet: Briefly, CD25+ cells were depleted using LD columns and a
Techniques: Expressing, Flow Cytometry, Cytometry