columns Search Results


acsa 2 microbeads  (Miltenyi Biotec)
99
Miltenyi Biotec acsa 2 microbeads
Acsa 2 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gravity columns  (TaKaRa)
95
TaKaRa gravity columns
Gravity Columns, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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b cells  (Miltenyi Biotec)
99
Miltenyi Biotec b cells
B Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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macsquant columns  (Miltenyi Biotec)
94
Miltenyi Biotec macsquant columns
Macsquant Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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automacs  (Miltenyi Biotec)
95
Miltenyi Biotec automacs
Automacs, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ms columns  (Miltenyi Biotec)
99
Miltenyi Biotec ms columns
Ms Columns, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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his60 nickel gravity column  (TaKaRa)
94
TaKaRa his60 nickel gravity column
His60 Nickel Gravity Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nickel spin columns  (New England Biolabs)
95
New England Biolabs nickel spin columns
Nickel Spin Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gdna removal columns  (New England Biolabs)
93
New England Biolabs gdna removal columns
Gdna Removal Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cation exchange column macro prep high s  (Bio-Rad)
93
Bio-Rad cation exchange column macro prep high s
Cation Exchange Column Macro Prep High S, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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columns  (Repligen Corp)
94
Repligen Corp columns
Columns, supplied by Repligen Corp, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd25 depletion kit  (Miltenyi Biotec)
99
Miltenyi Biotec cd25 depletion kit
Figure 3. MAZR Affects Thymic Treg Cell Development (A) Dot plots depict <t>CD25</t> and FOXP3 expression in WT and MAZR-cKO CD4SP thymocytes. Diagram (right) shows the percentages of CD25–FOXP3–, CD25–FOXP3+, and CD25+FOXP3+ cells, gated on CD4SP thymocytes, in WT and MAZR-cKO mice. (B) Flow cytometry analysis shows MAZR expression in the indicated T cell subsets. The colored histograms show cells pre-gated on the four WT CD25 and FOXP3 quadrants from (A), depicting four different stages of tTreg differentiation. (C) Expression of MAZR was assessed by flow cytometry in DN, DP, CD8SP, CD4SP, CD4+CD25–FOXP3+ (Treg precursors), and CD4+CD25+FOXP3+ (Treg) subsets in the thymus as well as splenic CD8+CD44–CD62Lhi (naive CD8), CD8+CD44+CD62L– (effector CD8), CD4+CD44–CD62Lhi (naive CD4), CD4+CD44+CD62L– (effector CD4), and CD4+FOXP3+ (Treg) subsets.
Cd25 Depletion Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. MAZR Affects Thymic Treg Cell Development (A) Dot plots depict CD25 and FOXP3 expression in WT and MAZR-cKO CD4SP thymocytes. Diagram (right) shows the percentages of CD25–FOXP3–, CD25–FOXP3+, and CD25+FOXP3+ cells, gated on CD4SP thymocytes, in WT and MAZR-cKO mice. (B) Flow cytometry analysis shows MAZR expression in the indicated T cell subsets. The colored histograms show cells pre-gated on the four WT CD25 and FOXP3 quadrants from (A), depicting four different stages of tTreg differentiation. (C) Expression of MAZR was assessed by flow cytometry in DN, DP, CD8SP, CD4SP, CD4+CD25–FOXP3+ (Treg precursors), and CD4+CD25+FOXP3+ (Treg) subsets in the thymus as well as splenic CD8+CD44–CD62Lhi (naive CD8), CD8+CD44+CD62L– (effector CD8), CD4+CD44–CD62Lhi (naive CD4), CD4+CD44+CD62L– (effector CD4), and CD4+FOXP3+ (Treg) subsets.

Journal: Cell reports

Article Title: The Transcription Factor MAZR/PATZ1 Regulates the Development of FOXP3 + Regulatory T Cells.

doi: 10.1016/j.celrep.2019.11.089

Figure Lengend Snippet: Figure 3. MAZR Affects Thymic Treg Cell Development (A) Dot plots depict CD25 and FOXP3 expression in WT and MAZR-cKO CD4SP thymocytes. Diagram (right) shows the percentages of CD25–FOXP3–, CD25–FOXP3+, and CD25+FOXP3+ cells, gated on CD4SP thymocytes, in WT and MAZR-cKO mice. (B) Flow cytometry analysis shows MAZR expression in the indicated T cell subsets. The colored histograms show cells pre-gated on the four WT CD25 and FOXP3 quadrants from (A), depicting four different stages of tTreg differentiation. (C) Expression of MAZR was assessed by flow cytometry in DN, DP, CD8SP, CD4SP, CD4+CD25–FOXP3+ (Treg precursors), and CD4+CD25+FOXP3+ (Treg) subsets in the thymus as well as splenic CD8+CD44–CD62Lhi (naive CD8), CD8+CD44+CD62L– (effector CD8), CD4+CD44–CD62Lhi (naive CD4), CD4+CD44+CD62L– (effector CD4), and CD4+FOXP3+ (Treg) subsets.

Article Snippet: Briefly, CD25+ cells were depleted using LD columns and a CD25 depletion kit (Miltenyi Biotec).

Techniques: Expressing, Flow Cytometry, Cytometry