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Image Search Results
Journal: Toxins
Article Title: Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process
doi: 10.3390/toxins2061318
Figure Lengend Snippet: Localization of Stx and StxB to the nucleoli of intestinal epithelial T84 cells and human colonic tissue. ( a ) Stx-Alexa 488 (green) appears in the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye; arrows point to the nucleoli filled with Stx-Alexa 488. ( b ) Recombinant StxB-Alexa 568 (red) is delivered from cytoplasm into the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye. ( c ) T84 cells immunostained with an antibody against the nucleolar protein nucleolin (red). ( d ) Same cells as in c stained with StxB-Alexa 488 (green), confirming StxB nucleolar localization. ( e ) Normal human colonic tissue immunostained with StxB-Alexa 488 (green) indicates the presence of StxB binding sites in nucleoli of crypt epithelial cells; red: β-catenin; blue: Hoechst nuclear stain; arrows in the magnified insert point to the nucleoli inside the nuclei filled with StxB-Alexa 488. ( f ) StxB-Alexa 568 (red) is accumulated in the nucleoli of PM-permeabilized T84 cells, while CTB-fluorescein (green) is present in cytoplasm and membranes but not in nucleoli.
Article Snippet:
Techniques: Staining, Recombinant, Binding Assay