colon normal tissue Search Results


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Indivumed gmbh tissue microarrays containing colonic or rectal adenocarcinoma and normal tissue adjacent to tumor
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Cooperative Human Tissue Network (CHTN normal colon specimen
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Johns Hopkins HealthCare frozen thick samples of surgically removed, normal human colonic tissue
Localization of Stx and StxB to the nucleoli of intestinal epithelial T84 cells and <t>human</t> <t>colonic</t> <t>tissue.</t> ( a ) Stx-Alexa 488 (green) appears in the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye; arrows point to the nucleoli filled with Stx-Alexa 488. ( b ) Recombinant StxB-Alexa 568 (red) is delivered from cytoplasm into the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye. ( c ) T84 cells immunostained with an antibody against the nucleolar protein nucleolin (red). ( d ) Same cells as in c stained with StxB-Alexa 488 (green), confirming StxB nucleolar localization. ( e ) <t>Normal</t> human colonic tissue immunostained with StxB-Alexa 488 (green) indicates the presence of StxB binding sites in nucleoli of crypt epithelial cells; red: β-catenin; blue: Hoechst nuclear stain; arrows in the magnified insert point to the nucleoli inside the nuclei filled with StxB-Alexa 488. ( f ) StxB-Alexa 568 (red) is accumulated in the nucleoli of PM-permeabilized T84 cells, while CTB-fluorescein (green) is present in cytoplasm and membranes but not in nucleoli.
Frozen Thick Samples Of Surgically Removed, Normal Human Colonic Tissue, supplied by Johns Hopkins HealthCare, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TissueArray.com LLC colon adenocarcinoma tissue microarray with matched adjacent normal tissue 11
Localization of Stx and StxB to the nucleoli of intestinal epithelial T84 cells and <t>human</t> <t>colonic</t> <t>tissue.</t> ( a ) Stx-Alexa 488 (green) appears in the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye; arrows point to the nucleoli filled with Stx-Alexa 488. ( b ) Recombinant StxB-Alexa 568 (red) is delivered from cytoplasm into the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye. ( c ) T84 cells immunostained with an antibody against the nucleolar protein nucleolin (red). ( d ) Same cells as in c stained with StxB-Alexa 488 (green), confirming StxB nucleolar localization. ( e ) <t>Normal</t> human colonic tissue immunostained with StxB-Alexa 488 (green) indicates the presence of StxB binding sites in nucleoli of crypt epithelial cells; red: β-catenin; blue: Hoechst nuclear stain; arrows in the magnified insert point to the nucleoli inside the nuclei filled with StxB-Alexa 488. ( f ) StxB-Alexa 568 (red) is accumulated in the nucleoli of PM-permeabilized T84 cells, while CTB-fluorescein (green) is present in cytoplasm and membranes but not in nucleoli.
Colon Adenocarcinoma Tissue Microarray With Matched Adjacent Normal Tissue 11, supplied by TissueArray.com LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Localization of Stx and StxB to the nucleoli of intestinal epithelial T84 cells and human colonic tissue. ( a ) Stx-Alexa 488 (green) appears in the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye; arrows point to the nucleoli filled with Stx-Alexa 488. ( b ) Recombinant StxB-Alexa 568 (red) is delivered from cytoplasm into the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye. ( c ) T84 cells immunostained with an antibody against the nucleolar protein nucleolin (red). ( d ) Same cells as in c stained with StxB-Alexa 488 (green), confirming StxB nucleolar localization. ( e ) Normal human colonic tissue immunostained with StxB-Alexa 488 (green) indicates the presence of StxB binding sites in nucleoli of crypt epithelial cells; red: β-catenin; blue: Hoechst nuclear stain; arrows in the magnified insert point to the nucleoli inside the nuclei filled with StxB-Alexa 488. ( f ) StxB-Alexa 568 (red) is accumulated in the nucleoli of PM-permeabilized T84 cells, while CTB-fluorescein (green) is present in cytoplasm and membranes but not in nucleoli.

Journal: Toxins

Article Title: Shiga Toxin Is Transported into the Nucleoli of Intestinal Epithelial Cells via a Carrier-Dependent Process

doi: 10.3390/toxins2061318

Figure Lengend Snippet: Localization of Stx and StxB to the nucleoli of intestinal epithelial T84 cells and human colonic tissue. ( a ) Stx-Alexa 488 (green) appears in the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye; arrows point to the nucleoli filled with Stx-Alexa 488. ( b ) Recombinant StxB-Alexa 568 (red) is delivered from cytoplasm into the nucleoli of PM-permeabilized confluent T84 cells; blue: nuclear staining by Hoechst dye. ( c ) T84 cells immunostained with an antibody against the nucleolar protein nucleolin (red). ( d ) Same cells as in c stained with StxB-Alexa 488 (green), confirming StxB nucleolar localization. ( e ) Normal human colonic tissue immunostained with StxB-Alexa 488 (green) indicates the presence of StxB binding sites in nucleoli of crypt epithelial cells; red: β-catenin; blue: Hoechst nuclear stain; arrows in the magnified insert point to the nucleoli inside the nuclei filled with StxB-Alexa 488. ( f ) StxB-Alexa 568 (red) is accumulated in the nucleoli of PM-permeabilized T84 cells, while CTB-fluorescein (green) is present in cytoplasm and membranes but not in nucleoli.

Article Snippet: Human normal colonic tissue: Frozen 20 μm thick samples of surgically removed, normal human colonic tissue were obtained from the Johns Hopkins Colorectal Cancer Registry with JHM-IRB1 granted exemption.

Techniques: Staining, Recombinant, Binding Assay