Journal: British Journal of Cancer

Article Title: SKCG-1 : a new candidate growth regulatory gene at chromosome 11q23.2 in human sporadic Wilms tumours

doi: 10.1038/sj.bjc.6603090

Figure Lengend Snippet: Full-length cDNA sequence and deduced amino-acid sequence of SKCG-1 . The full-length SKCG-1 cDNA encodes a predicted polypeptide of 124 amino acids. The polyadenylation signal is underlined. Sequence has been submitted to the NCBI database and the assigned GenBank accession number for SKCG1 is AY662656.

Article Snippet: The expression of SKCG-1 gene among the various tissues was analysed by PCR using tissue-specific cDNA panel (OriGene Technologies).

Techniques: Sequencing

Journal: British Journal of Cancer

Article Title: SKCG-1 : a new candidate growth regulatory gene at chromosome 11q23.2 in human sporadic Wilms tumours

doi: 10.1038/sj.bjc.6603090

Figure Lengend Snippet: Distribution of SKCG-1 gene expression in various human tissues. Expression of SKCG-1 gene among the various tissues analysed by SKCG-1 gene-specific PCR using tissue-specific cDNA panel (OriGene Technologies). Arrow indicates the expected size (300 bp) PCR product of SKCG-1 from tissue-specific cDNA.

Article Snippet: The expression of SKCG-1 gene among the various tissues was analysed by PCR using tissue-specific cDNA panel (OriGene Technologies).

Techniques: Gene Expression, Expressing

Journal: British Journal of Cancer

Article Title: SKCG-1 : a new candidate growth regulatory gene at chromosome 11q23.2 in human sporadic Wilms tumours

doi: 10.1038/sj.bjc.6603090

Figure Lengend Snippet: Sequence alignment at cDNA level of SKCG-1 with a solute carrier family 14 (urea transporter) gene ( A ), and SOS2-like protein kinase mRNA ( B ).

Article Snippet: The expression of SKCG-1 gene among the various tissues was analysed by PCR using tissue-specific cDNA panel (OriGene Technologies).

Techniques: Sequencing

Journal: Cell Death & Disease

Article Title: Human BCL-G regulates secretion of inflammatory chemokines but is dispensable for induction of apoptosis by IFN-γ and TNF-α in intestinal epithelial cells

doi: 10.1038/s41419-020-2263-0

Figure Lengend Snippet: a Relative mRNA expression of BCL-G S/L measured by RT-qPCR in the indicated adult human tissues. Relative mRNA expression of b BCL-G S and c BCL-G L measured by RT-qPCR in colonic biopsy tissues isolated from non-IBD individuals ( n = 20), patients with inactive ( n = 20) or active ( n = 24) ulcerative colitis, and patients with inactive ( n = 19) or active ( n = 21) Crohn’s disease. BCL-G S/L expression data in active ulcerative colitis were used for correlation analysis in Fig. . For panels ( b ) and ( c ), data shown include the median with interquartile range. d Relative mRNA expression of BCL-G S/L measured by RT-qPCR in TissueScan cDNA array of 24 matched samples (normal, uninvolved colon vs. colorectal cancer) covering clinical stage I ( n = 5), II ( n = 7), III ( n = 8) and IV ( n = 4). # p < 0.05, ### p < 0.001 (Kruskal–Wallis test followed by Dunn’s multiple comparisons test as indicated), $ p < 0.05, $$$ p < 0.001 (repeated measures two-way ANOVA followed by Fisher’s LSD test as indicated), ‡‡ p < 0.01, ‡‡‡ p < 0.001 (repeated measures two-way ANOVA followed by Fisher’s LSD test vs. normal colon, stage I). IBD — inflammatory bowel disease, UC — ulcerative colitis, CD — Crohn’s disease.

Article Snippet: To study cancer-associated gene expression, Colon Cancer TissueScan cDNA array III (cat# HCRT303, OriGene) was used.

Techniques: Expressing, Quantitative RT-PCR, Isolation