Journal: mAbs

Article Title: Insertion of scFv into the hinge domain of full-length IgG1 monoclonal antibody results in tetravalent bispecific molecule with robust properties

doi: 10.1080/19420862.2016.1270492

Figure Lengend Snippet: Tumor growth inhibition of Bs4Ab-VA, anti-VEGF and anti-Ang2, and their combination using a colon cancer (Colo205) mouse xenograft model. When the tumor reached a volume of approximately 200 mm3, mice (n = 6) were administered intraperitoneally twice per week for four weeks with 14 mg/kg of Bs4Ab-VA, 10 mg/kg of anti-VEGF, anti-Ang2, their combination, and a non-binding isotype antibody. Untreated mice were used as control. 10 mg/kg of each of the two parental antibodies were used for the combination study.

Article Snippet: 5 × 10 6 Colo205 cells in 50% matrigel were inoculated subcutaneously on the right flank into 5- to 6-weeks old female athymic nude mice (Harlan Laboratories).

Techniques: Inhibition, Binding Assay